DNA nanostructure‐encoded fluorescent barcodes
Abstract Identification and differentiation of various bioanalytes with high spatial and temporal resolution have spawned fluorescent barcode‐based detection and imaging. DNA nanostructures with excellent structure programmability, addressability, and near‐atomic structure accuracy have emerged as a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-12-01
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| Series: | Aggregate |
| Online Access: | https://doi.org/10.1002/agt2.8 |
| _version_ | 1819180403407192064 |
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| author | Tingting Zhai Qian Li Jianlei Shen Jiang Li Chunhai Fan |
| author_facet | Tingting Zhai Qian Li Jianlei Shen Jiang Li Chunhai Fan |
| author_sort | Tingting Zhai |
| collection | DOAJ |
| description | Abstract Identification and differentiation of various bioanalytes with high spatial and temporal resolution have spawned fluorescent barcode‐based detection and imaging. DNA nanostructures with excellent structure programmability, addressability, and near‐atomic structure accuracy have emerged as a versatile platform to develop fluorescent barcodes through finely controlled dye numbers, relative distances, and compositions. A variety of DNA fluorescent barcodes with distinguishable spectral colors, geometries and digitized fluorescence intensities have been constructed and favorably implemented in the field of bioimaging, multiplex bioassay, and information security. In this review, we first summarize the state of the art of self‐assembled DNA nanostructures. Next, the utilization of DNA nanostructure to develop fluorescent barcodes and the photophysical properties of DNA‐templated fluorophores are reviewed. Finally, the applications of DNA fluorescent barcodes for biosensing and imaging, and the challenges and outlook of DNA nanostructure‐engineered fluorescent barcodes are discussed. |
| first_indexed | 2024-12-22T22:13:47Z |
| format | Article |
| id | doaj.art-1327d3307f9244f28a6579c2a1582840 |
| institution | Directory Open Access Journal |
| issn | 2692-4560 |
| language | English |
| last_indexed | 2024-12-22T22:13:47Z |
| publishDate | 2020-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aggregate |
| spelling | doaj.art-1327d3307f9244f28a6579c2a15828402022-12-21T18:10:49ZengWileyAggregate2692-45602020-12-011110711610.1002/agt2.8DNA nanostructure‐encoded fluorescent barcodesTingting Zhai0Qian Li1Jianlei Shen2Jiang Li3Chunhai Fan4Institute of Molecular Medicine Shanghai Key Laboratory for Nucleic Acids Chemistry and Nanomedicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai ChinaSchool of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine Shanghai Jiao Tong University Shanghai ChinaSchool of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine Shanghai Jiao Tong University Shanghai ChinaBioimaging Center Shanghai Synchrotron Radiation Facility Zhangjiang Laboratory Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai ChinaInstitute of Molecular Medicine Shanghai Key Laboratory for Nucleic Acids Chemistry and Nanomedicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai ChinaAbstract Identification and differentiation of various bioanalytes with high spatial and temporal resolution have spawned fluorescent barcode‐based detection and imaging. DNA nanostructures with excellent structure programmability, addressability, and near‐atomic structure accuracy have emerged as a versatile platform to develop fluorescent barcodes through finely controlled dye numbers, relative distances, and compositions. A variety of DNA fluorescent barcodes with distinguishable spectral colors, geometries and digitized fluorescence intensities have been constructed and favorably implemented in the field of bioimaging, multiplex bioassay, and information security. In this review, we first summarize the state of the art of self‐assembled DNA nanostructures. Next, the utilization of DNA nanostructure to develop fluorescent barcodes and the photophysical properties of DNA‐templated fluorophores are reviewed. Finally, the applications of DNA fluorescent barcodes for biosensing and imaging, and the challenges and outlook of DNA nanostructure‐engineered fluorescent barcodes are discussed.https://doi.org/10.1002/agt2.8 |
| spellingShingle | Tingting Zhai Qian Li Jianlei Shen Jiang Li Chunhai Fan DNA nanostructure‐encoded fluorescent barcodes Aggregate |
| title | DNA nanostructure‐encoded fluorescent barcodes |
| title_full | DNA nanostructure‐encoded fluorescent barcodes |
| title_fullStr | DNA nanostructure‐encoded fluorescent barcodes |
| title_full_unstemmed | DNA nanostructure‐encoded fluorescent barcodes |
| title_short | DNA nanostructure‐encoded fluorescent barcodes |
| title_sort | dna nanostructure encoded fluorescent barcodes |
| url | https://doi.org/10.1002/agt2.8 |
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