A Multiplex RT-PCR Assay for S. aureus, L. monocytogenes, and Salmonella spp. Detection in Raw Milk with Pre-enrichment

A Multiplex RT-PCR Assay for S. aureus, L. monocytogenes, and Salmonella spp. Detection in Raw Milk with Pre-enrichment

This study firstly developed a multiplex real-time PCR (RT-PCR) technique combined with a pre-enrichment step to simultaneously detect Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw milk and the dairy farm environment (feces, soil, feed, water...

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Bibliographic Details
Main Authors:	Tian Ding, Yuanjie Suo, Zhaohuan Zhang, Donghong Liu, Xingqian Ye, Shiguo Chen, Yong Zhao
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2017-05-01
Series:	Frontiers in Microbiology
Subjects:	multiplex real-time PCR enrichment step S. aureus L. monocytogenes Salmonella spp. dairy farm environment
Online Access:	http://journal.frontiersin.org/article/10.3389/fmicb.2017.00989/full

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