Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for Shrimp
The white spot syndrome virus (WSSV) is the causative agent of white spot disease, which kills shrimp within a few days of infection. Although WSSV has a mortality rate of almost 100% and poses a serious threat to the shrimp farming industry, strategies for its prevention and treatment are extremely...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/1999-4915/15/10/2010 |
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| author | Min-Jeong Kim Su-Hyun Kim Jong-Oh Kim Taek-Kyun Lee In-Kwon Jang Tae-Jin Choi |
| author_facet | Min-Jeong Kim Su-Hyun Kim Jong-Oh Kim Taek-Kyun Lee In-Kwon Jang Tae-Jin Choi |
| author_sort | Min-Jeong Kim |
| collection | DOAJ |
| description | The white spot syndrome virus (WSSV) is the causative agent of white spot disease, which kills shrimp within a few days of infection. Although WSSV has a mortality rate of almost 100% and poses a serious threat to the shrimp farming industry, strategies for its prevention and treatment are extremely limited. In this study, we examined the efficacy of VP28, a recombinant WSSV protein expressed in <i>Chlorella vulgaris</i> (<i>C. vulgaris</i>), as an oral shrimp vaccine. When compared with the control group, in which WSSV had a cumulative mortality of 100%, shrimp treated with 5% VP28-expressing <i>C. vulgaris</i> in their feed only had a 20% cumulative mortality rate 12 days after the WSSV challenge. When compared with the nonvaccinated group, the transcription of anti-lipopolysaccharide factor, C-type lectin, and prophenoloxidase genes, which are involved in shrimp defense against WSSV infection, was upregulated 29.6 fold, 15.4 fold, and 11.5 fold, respectively. These findings highlight <i>C</i>. <i>vulgaris</i> as a potential host for industrial shrimp vaccine production. |
| first_indexed | 2024-03-10T20:49:31Z |
| format | Article |
| id | doaj.art-132baee3b8ad4650b727a6b5b4b04700 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:49:31Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-132baee3b8ad4650b727a6b5b4b047002023-11-19T18:26:53ZengMDPI AGViruses1999-49152023-09-011510201010.3390/v15102010Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for ShrimpMin-Jeong Kim0Su-Hyun Kim1Jong-Oh Kim2Taek-Kyun Lee3In-Kwon Jang4Tae-Jin Choi5Department of Microbiology, School of Marine and Fisheries Sciences, Pukyong National University, Busan 48513, Republic of KoreaDepartment of Microbiology, School of Marine and Fisheries Sciences, Pukyong National University, Busan 48513, Republic of KoreaDepartment of Microbiology, School of Marine and Fisheries Sciences, Pukyong National University, Busan 48513, Republic of KoreaSouth Sea Environment Research Division, Korea Institute of Ocean Science & Technology, Geoje-si 53201, Republic of KoreaJunggyeom Co., Ltd., Goyang-si 10223, Republic of KoreaDepartment of Microbiology, School of Marine and Fisheries Sciences, Pukyong National University, Busan 48513, Republic of KoreaThe white spot syndrome virus (WSSV) is the causative agent of white spot disease, which kills shrimp within a few days of infection. Although WSSV has a mortality rate of almost 100% and poses a serious threat to the shrimp farming industry, strategies for its prevention and treatment are extremely limited. In this study, we examined the efficacy of VP28, a recombinant WSSV protein expressed in <i>Chlorella vulgaris</i> (<i>C. vulgaris</i>), as an oral shrimp vaccine. When compared with the control group, in which WSSV had a cumulative mortality of 100%, shrimp treated with 5% VP28-expressing <i>C. vulgaris</i> in their feed only had a 20% cumulative mortality rate 12 days after the WSSV challenge. When compared with the nonvaccinated group, the transcription of anti-lipopolysaccharide factor, C-type lectin, and prophenoloxidase genes, which are involved in shrimp defense against WSSV infection, was upregulated 29.6 fold, 15.4 fold, and 11.5 fold, respectively. These findings highlight <i>C</i>. <i>vulgaris</i> as a potential host for industrial shrimp vaccine production.https://www.mdpi.com/1999-4915/15/10/2010WSSVVP28vaccine<i>Chlorella vulgaris</i>immunization |
| spellingShingle | Min-Jeong Kim Su-Hyun Kim Jong-Oh Kim Taek-Kyun Lee In-Kwon Jang Tae-Jin Choi Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for Shrimp Viruses WSSV VP28 vaccine <i>Chlorella vulgaris</i> immunization |
| title | Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for Shrimp |
| title_full | Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for Shrimp |
| title_fullStr | Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for Shrimp |
| title_full_unstemmed | Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for Shrimp |
| title_short | Efficacy of White Spot Syndrome Virus Protein VP28-Expressing <i>Chlorella vulgaris</i> as an Oral Vaccine for Shrimp |
| title_sort | efficacy of white spot syndrome virus protein vp28 expressing i chlorella vulgaris i as an oral vaccine for shrimp |
| topic | WSSV VP28 vaccine <i>Chlorella vulgaris</i> immunization |
| url | https://www.mdpi.com/1999-4915/15/10/2010 |
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