Cytotoxic Indole Diterpenoids from a <i>Sphagneticola trilobata</i>-Derived Fungus <i>Aspergillus</i> sp. PQJ-1

Two new indole diterpene derivatives, 5<i>S</i>-hydroxy-<i>β</i>-aflatrem (<b>1</b>) and 14<i>R</i>-hydroxy-<i>β</i>-aflatrem (<b>2</b>), along with one known analogue, 14-(<i>N</i>,<i>N</i>-dimethl-<i...

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Bibliographic Details
Main Authors: Wenxing Li, Guohui Yi, Kaiwen Lin, Guangying Chen, Yang Hui, Wenhao Chen
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/28/20/7003
Description
Summary:Two new indole diterpene derivatives, 5<i>S</i>-hydroxy-<i>β</i>-aflatrem (<b>1</b>) and 14<i>R</i>-hydroxy-<i>β</i>-aflatrem (<b>2</b>), along with one known analogue, 14-(<i>N</i>,<i>N</i>-dimethl-<i>L</i>-valyloxy)paspalinine (<b>3</b>), were isolated from the fermentation broth of the fungus <i>Aspergillus</i> sp. PQJ-1 derived from <i>Sphagneticola trilobata</i>. The structures of the new compounds were elucidated from spectroscopic data and ECD spectroscopic analyses. All the compounds (<b>1</b>–<b>3</b>) were evaluated for their cytotoxicity against A549, Hela, Hep G2, and MCF-7 cell lines. Compounds <b>1</b> and <b>2</b> exhibited selective inhibition against Hela cells. Further studies showed that <b>1</b> significantly induced apoptosis and suppressed migration and invasion in Hela cells. Moreover, <b>1</b> could up-regulate pro-apoptotic genes BAX and Caspase-3 and down-regulate anti-apoptotic genes Bcl-xL and XIXP.
ISSN:1420-3049