Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities
Abstract Eucalyptus species possess anti-inflammatory, antifungal, antibacterial, and insecticidal properties. In this study, the chemical composition and biological activities of Eucalyptus cinerea essential oil (EO) and the leaf and stem anatomy were investigated. EO was extracted by Clevenger app...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2021-07-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000200206&tlng=en |
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| author | Matheus Saukoski Pauzer Thais de Oliveira Borsato Valter Paes de Almeida Vijayasankar Raman Bárbara Justus Camila Bugnotto Pereira Thiago Bevilacqua Flores Beatriz Helena Lameiro Noronha Sales Maia Emanuelle Kretz Meneghetti Carla Cristine Kanunfre Josiane de Fátima Padilha de Paula Paulo Vitor Farago Jane Manfron Budel |
| author_facet | Matheus Saukoski Pauzer Thais de Oliveira Borsato Valter Paes de Almeida Vijayasankar Raman Bárbara Justus Camila Bugnotto Pereira Thiago Bevilacqua Flores Beatriz Helena Lameiro Noronha Sales Maia Emanuelle Kretz Meneghetti Carla Cristine Kanunfre Josiane de Fátima Padilha de Paula Paulo Vitor Farago Jane Manfron Budel |
| author_sort | Matheus Saukoski Pauzer |
| collection | DOAJ |
| description | Abstract Eucalyptus species possess anti-inflammatory, antifungal, antibacterial, and insecticidal properties. In this study, the chemical composition and biological activities of Eucalyptus cinerea essential oil (EO) and the leaf and stem anatomy were investigated. EO was extracted by Clevenger apparatus and the compounds were identified by GC/MS. The antioxidant activity was evaluated by DPPH, ABTS, and reducing phosphomolybdenum complex. Broth microdilution was used to determine antimicrobial activity. Cytotoxicity was verified against HeLa, HRT-18, and Calu-3 cells by MTT assay. The cytotoxic mechanism was studied by cell DNA content, cell cycle, and DNA fragmentation. The microscopic analyzes of the leaves and the stems were performed by light microscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The main constituent of the EO was 1,8-cineole (55.24%). The EO showed low antioxidant and antimicrobial activities. Calu-3 cells showed a significant reduction in viability with IC50 of 689.79 ± 29.34 μg/mL. EO at 1000 μg/mL decreased the DNA content in Jurkat cells. In general, EO increased cell percentage in sub-G0 and S phases with concomitant reduction of cell percentage in G0/G1 and G2/M phases and provided DNA fragmentation of 29.73%. Anatomical and micromorphological features of the leaves and stems can help in the species identification and its differentiation from other Eucalyptus species. |
| first_indexed | 2024-12-20T15:19:20Z |
| format | Article |
| id | doaj.art-132dbdf7add4467181ec3fc1b7c4569a |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-20T15:19:20Z |
| publishDate | 2021-07-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-132dbdf7add4467181ec3fc1b7c4569a2022-12-21T19:36:07ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242021-07-0164spe10.1590/1678-4324-75years-2021200772Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic ActivitiesMatheus Saukoski Pauzerhttps://orcid.org/0000-0002-3348-0130Thais de Oliveira Borsatohttps://orcid.org/0000-0002-1300-8162Valter Paes de Almeidahttps://orcid.org/0000-0003-0603-8184Vijayasankar Ramanhttps://orcid.org/0000-0001-7368-9644Bárbara Justushttps://orcid.org/0000-0003-3943-9304Camila Bugnotto Pereirahttps://orcid.org/0000-0001-8210-4804Thiago Bevilacqua Floreshttps://orcid.org/0000-0003-3015-9154Beatriz Helena Lameiro Noronha Sales Maiahttps://orcid.org/0000-0001-5896-2892Emanuelle Kretz Meneghettihttps://orcid.org/0000-0001-8988-4073Carla Cristine Kanunfrehttps://orcid.org/0000-0002-2865-3084Josiane de Fátima Padilha de Paulahttps://orcid.org/0000-0002-2717-8867Paulo Vitor Faragohttps://orcid.org/0000-0002-9934-4027Jane Manfron Budelhttps://orcid.org/0000-0003-1873-2253Abstract Eucalyptus species possess anti-inflammatory, antifungal, antibacterial, and insecticidal properties. In this study, the chemical composition and biological activities of Eucalyptus cinerea essential oil (EO) and the leaf and stem anatomy were investigated. EO was extracted by Clevenger apparatus and the compounds were identified by GC/MS. The antioxidant activity was evaluated by DPPH, ABTS, and reducing phosphomolybdenum complex. Broth microdilution was used to determine antimicrobial activity. Cytotoxicity was verified against HeLa, HRT-18, and Calu-3 cells by MTT assay. The cytotoxic mechanism was studied by cell DNA content, cell cycle, and DNA fragmentation. The microscopic analyzes of the leaves and the stems were performed by light microscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The main constituent of the EO was 1,8-cineole (55.24%). The EO showed low antioxidant and antimicrobial activities. Calu-3 cells showed a significant reduction in viability with IC50 of 689.79 ± 29.34 μg/mL. EO at 1000 μg/mL decreased the DNA content in Jurkat cells. In general, EO increased cell percentage in sub-G0 and S phases with concomitant reduction of cell percentage in G0/G1 and G2/M phases and provided DNA fragmentation of 29.73%. Anatomical and micromorphological features of the leaves and stems can help in the species identification and its differentiation from other Eucalyptus species.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000200206&tlng=enanatomybiological activityhistochemistrymicroscopyMyrtaceaeterpenoidsvolatile oils |
| spellingShingle | Matheus Saukoski Pauzer Thais de Oliveira Borsato Valter Paes de Almeida Vijayasankar Raman Bárbara Justus Camila Bugnotto Pereira Thiago Bevilacqua Flores Beatriz Helena Lameiro Noronha Sales Maia Emanuelle Kretz Meneghetti Carla Cristine Kanunfre Josiane de Fátima Padilha de Paula Paulo Vitor Farago Jane Manfron Budel Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities Brazilian Archives of Biology and Technology anatomy biological activity histochemistry microscopy Myrtaceae terpenoids volatile oils |
| title | Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities |
| title_full | Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities |
| title_fullStr | Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities |
| title_full_unstemmed | Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities |
| title_short | Eucalyptus cinerea: Microscopic Profile, Chemical Composition of Essential Oil and its Antioxidant, Microbiological and Cytotoxic Activities |
| title_sort | eucalyptus cinerea microscopic profile chemical composition of essential oil and its antioxidant microbiological and cytotoxic activities |
| topic | anatomy biological activity histochemistry microscopy Myrtaceae terpenoids volatile oils |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000200206&tlng=en |
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