SerpinB3 administration protects liver against ischemia-reperfusion injury
We have investigated the change in SerpinB3 during hepatic ischemia and the potential role of its antiprotease activity in cell protection by the administration of wild-type SerpinB3 (SerpinB3-WT) or active loop-deleted recombinant SerpinB3 protein (SerpinB3-D) in a rat model of ischemia (60 min)/r...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2022-10-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/3561 |
| _version_ | 1811197046947840000 |
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| author | Cristian Turato Mariapia Vairetti Marta Cagna Alessandra Biasiolo Andrea Ferrigno Santina Quarta Mariagrazia Ruvoletto Silvia De Siervi Patrizia Pontisso Laura Giuseppina Di Pasqua |
| author_facet | Cristian Turato Mariapia Vairetti Marta Cagna Alessandra Biasiolo Andrea Ferrigno Santina Quarta Mariagrazia Ruvoletto Silvia De Siervi Patrizia Pontisso Laura Giuseppina Di Pasqua |
| author_sort | Cristian Turato |
| collection | DOAJ |
| description |
We have investigated the change in SerpinB3 during hepatic ischemia and the potential role of its antiprotease activity in cell protection by the administration of wild-type SerpinB3 (SerpinB3-WT) or active loop-deleted recombinant SerpinB3 protein (SerpinB3-D) in a rat model of ischemia (60 min)/reperfusion (60 min) (I/R). A time-dependent increase of SerpinB3, both at transcription and protein level, was found in ischemic livers after 60, 120 and 180 min. SerpinB3-WT decreased polymorphonuclear cell infiltration and serum enzymes and increased ATP when compared with I/R group. These events were not obtained using SerpinB3-D. No significant changes in both liver SerpinB3 mRNA and protein were found in all I/R groups considered. The present data show that the administration of SerpinB3-WT reduced the I/R injury and this effect appears to be dependent on its anti-protease activity.
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| first_indexed | 2024-04-12T01:08:37Z |
| format | Article |
| id | doaj.art-132dd393be9445019719bfe2e49b6ede |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-12T01:08:37Z |
| publishDate | 2022-10-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-132dd393be9445019719bfe2e49b6ede2022-12-22T03:54:10ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062022-10-0166410.4081/ejh.2022.3561SerpinB3 administration protects liver against ischemia-reperfusion injuryCristian Turato0Mariapia Vairetti1Marta Cagna2Alessandra Biasiolo3Andrea Ferrigno4Santina Quarta5Mariagrazia Ruvoletto6Silvia De Siervi7Patrizia Pontisso8Laura Giuseppina Di Pasqua9Department of Molecular Medicine, University of PaviaDepartmentt of Internal Medicine and Therapeutics, University of PaviaDepartment of Internal Medicine and Therapeutics, University of PaviaDepartment of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, PaduaDepartment of Internal Medicine and Therapeutics, University of PaviaDepartment of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, PaduaDepartment of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, PaduaDepartment of Molecular Medicine, University of PaviaDepartment of Medicine, University of Padua; LifeLab Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, PaduaDepartment of Internal Medicine and Therapeutics, University of Pavia We have investigated the change in SerpinB3 during hepatic ischemia and the potential role of its antiprotease activity in cell protection by the administration of wild-type SerpinB3 (SerpinB3-WT) or active loop-deleted recombinant SerpinB3 protein (SerpinB3-D) in a rat model of ischemia (60 min)/reperfusion (60 min) (I/R). A time-dependent increase of SerpinB3, both at transcription and protein level, was found in ischemic livers after 60, 120 and 180 min. SerpinB3-WT decreased polymorphonuclear cell infiltration and serum enzymes and increased ATP when compared with I/R group. These events were not obtained using SerpinB3-D. No significant changes in both liver SerpinB3 mRNA and protein were found in all I/R groups considered. The present data show that the administration of SerpinB3-WT reduced the I/R injury and this effect appears to be dependent on its anti-protease activity. https://www.ejh.it/index.php/ejh/article/view/3561Ischemia/reperfusionSerpinB3inflammation |
| spellingShingle | Cristian Turato Mariapia Vairetti Marta Cagna Alessandra Biasiolo Andrea Ferrigno Santina Quarta Mariagrazia Ruvoletto Silvia De Siervi Patrizia Pontisso Laura Giuseppina Di Pasqua SerpinB3 administration protects liver against ischemia-reperfusion injury European Journal of Histochemistry Ischemia/reperfusion SerpinB3 inflammation |
| title | SerpinB3 administration protects liver against ischemia-reperfusion injury |
| title_full | SerpinB3 administration protects liver against ischemia-reperfusion injury |
| title_fullStr | SerpinB3 administration protects liver against ischemia-reperfusion injury |
| title_full_unstemmed | SerpinB3 administration protects liver against ischemia-reperfusion injury |
| title_short | SerpinB3 administration protects liver against ischemia-reperfusion injury |
| title_sort | serpinb3 administration protects liver against ischemia reperfusion injury |
| topic | Ischemia/reperfusion SerpinB3 inflammation |
| url | https://www.ejh.it/index.php/ejh/article/view/3561 |
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