Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples
<i>Pseudomonas aeruginosa</i> causes urinary tract infections associated with catheters by forming biofilms on the surface of indwelling catheters. Therefore, controlling the spread of the bacteria is crucial to preventing its transmission in hospitals and the environment. Thus, our obje...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1345 |
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| author | Telma de Sousa Michel Hébraud Olimpia Alves Eliana Costa Luís Maltez José Eduardo Pereira Ângela Martins Gilberto Igrejas Patricia Poeta |
| author_facet | Telma de Sousa Michel Hébraud Olimpia Alves Eliana Costa Luís Maltez José Eduardo Pereira Ângela Martins Gilberto Igrejas Patricia Poeta |
| author_sort | Telma de Sousa |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> causes urinary tract infections associated with catheters by forming biofilms on the surface of indwelling catheters. Therefore, controlling the spread of the bacteria is crucial to preventing its transmission in hospitals and the environment. Thus, our objective was to determine the antibiotic susceptibility profiles of twenty-five <i>P. aeruginosa</i> isolates from UTIs at the Medical Center of Trás-os-Montes and Alto Douro (CHTMAD). Biofilm formation and motility are also virulence factors studied in this work. Out of the twenty-five <i>P. aeruginosa</i> isolates, 16% exhibited multidrug resistance, being resistant to at least three classes of antibiotics. However, the isolates showed a high prevalence of susceptibility to amikacin and tobramycin. Resistance to carbapenem antibiotics, essential for treating infections when other antibiotics fail, was low in this study, Notably, 92% of the isolates demonstrated intermediate sensitivity to ciprofloxacin, raising concerns about its efficacy in controlling the disease. Genotypic analysis revealed the presence of various β-lactamase genes, with class B metallo-β-lactamases (MBLs) being the most common. The <i>bla</i><sub>NDM</sub>, <i>bla<sub>S</sub></i><sub>PM</sub>, and <i>bla</i><sub>VIM-VIM2</sub> genes were detected in 16%, 60%, and 12% of the strains, respectively. The presence of these genes highlights the emerging threat of MBL-mediated resistance. Additionally, virulence gene analysis showed varying prevalence rates among the strains. The <i>exo</i>U gene, associated with cytotoxicity, was found in only one isolate, while other genes such as <i>exo</i>S, <i>exo</i>A, <i>exo</i>Y, and <i>exo</i>T had a high prevalence. The <i>tox</i>A and <i>las</i>B genes were present in all isolates, whereas the <i>las</i>A gene was absent. The presence of various virulence genes suggests the potential of these strains to cause severe infections. This pathogen demonstrated proficiency in producing biofilms, as 92% of the isolates were found to be capable of doing so. Currently, antibiotic resistance is one of the most serious public health problems, as options become inadequate with the continued emergence and spread of multidrug-resistant strains, combined with the high rate of biofilm production and the ease of dissemination. In conclusion, this study provides insights into the antibiotic resistance and virulence profiles of <i>P. aeruginosa</i> strains isolated from human urine infections, highlighting the need for continued surveillance and appropriate therapeutic approaches. |
| first_indexed | 2024-03-11T03:28:02Z |
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| id | doaj.art-132f9aefb4e04fdd8d559ee8baf7936f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:28:02Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-132f9aefb4e04fdd8d559ee8baf7936f2023-11-18T02:35:26ZengMDPI AGMicroorganisms2076-26072023-05-01115134510.3390/microorganisms11051345Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine SamplesTelma de Sousa0Michel Hébraud1Olimpia Alves2Eliana Costa3Luís Maltez4José Eduardo Pereira5Ângela Martins6Gilberto Igrejas7Patricia Poeta8Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalUniversité Clermont Auvergne, INRAE, UMR Microbiologie Environnement Digestif Santé (MEDiS), 60122 Saint-Genès-Champanelle, FranceMedical Centre of Trás-os-Montes and Alto Douro, Clinical Pathology Department, 5000-801 Vila Real, PortugalMedical Centre of Trás-os-Montes and Alto Douro, Clinical Pathology Department, 5000-801 Vila Real, PortugalVeterinary and Animal Research Centre (CECAV), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalVeterinary and Animal Research Centre (CECAV), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalDepartment of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal<i>Pseudomonas aeruginosa</i> causes urinary tract infections associated with catheters by forming biofilms on the surface of indwelling catheters. Therefore, controlling the spread of the bacteria is crucial to preventing its transmission in hospitals and the environment. Thus, our objective was to determine the antibiotic susceptibility profiles of twenty-five <i>P. aeruginosa</i> isolates from UTIs at the Medical Center of Trás-os-Montes and Alto Douro (CHTMAD). Biofilm formation and motility are also virulence factors studied in this work. Out of the twenty-five <i>P. aeruginosa</i> isolates, 16% exhibited multidrug resistance, being resistant to at least three classes of antibiotics. However, the isolates showed a high prevalence of susceptibility to amikacin and tobramycin. Resistance to carbapenem antibiotics, essential for treating infections when other antibiotics fail, was low in this study, Notably, 92% of the isolates demonstrated intermediate sensitivity to ciprofloxacin, raising concerns about its efficacy in controlling the disease. Genotypic analysis revealed the presence of various β-lactamase genes, with class B metallo-β-lactamases (MBLs) being the most common. The <i>bla</i><sub>NDM</sub>, <i>bla<sub>S</sub></i><sub>PM</sub>, and <i>bla</i><sub>VIM-VIM2</sub> genes were detected in 16%, 60%, and 12% of the strains, respectively. The presence of these genes highlights the emerging threat of MBL-mediated resistance. Additionally, virulence gene analysis showed varying prevalence rates among the strains. The <i>exo</i>U gene, associated with cytotoxicity, was found in only one isolate, while other genes such as <i>exo</i>S, <i>exo</i>A, <i>exo</i>Y, and <i>exo</i>T had a high prevalence. The <i>tox</i>A and <i>las</i>B genes were present in all isolates, whereas the <i>las</i>A gene was absent. The presence of various virulence genes suggests the potential of these strains to cause severe infections. This pathogen demonstrated proficiency in producing biofilms, as 92% of the isolates were found to be capable of doing so. Currently, antibiotic resistance is one of the most serious public health problems, as options become inadequate with the continued emergence and spread of multidrug-resistant strains, combined with the high rate of biofilm production and the ease of dissemination. In conclusion, this study provides insights into the antibiotic resistance and virulence profiles of <i>P. aeruginosa</i> strains isolated from human urine infections, highlighting the need for continued surveillance and appropriate therapeutic approaches.https://www.mdpi.com/2076-2607/11/5/1345<i>Pseudomonas aeruginosa</i>biofilmmotilityantibiotic resistancevirulence |
| spellingShingle | Telma de Sousa Michel Hébraud Olimpia Alves Eliana Costa Luís Maltez José Eduardo Pereira Ângela Martins Gilberto Igrejas Patricia Poeta Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples Microorganisms <i>Pseudomonas aeruginosa</i> biofilm motility antibiotic resistance virulence |
| title | Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples |
| title_full | Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples |
| title_fullStr | Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples |
| title_full_unstemmed | Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples |
| title_short | Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples |
| title_sort | study of antimicrobial resistance biofilm formation and motility of i pseudomonas aeruginosa i derived from urine samples |
| topic | <i>Pseudomonas aeruginosa</i> biofilm motility antibiotic resistance virulence |
| url | https://www.mdpi.com/2076-2607/11/5/1345 |
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