Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings
Herein, polydopamine (PDA)-based antimicrobial coatings loaded with silver nanoparticles (Ag NPs) and gentamicin were designed and prepared on glass slides using two different approaches. To our knowledge, this study was performed for the first time with the aim to compare these methods (viz., in si...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/11/4258 |
| _version_ | 1827739406367195136 |
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| author | Rahila Batul Mrinal Bhave Aimin Yu |
| author_facet | Rahila Batul Mrinal Bhave Aimin Yu |
| author_sort | Rahila Batul |
| collection | DOAJ |
| description | Herein, polydopamine (PDA)-based antimicrobial coatings loaded with silver nanoparticles (Ag NPs) and gentamicin were designed and prepared on glass slides using two different approaches. To our knowledge, this study was performed for the first time with the aim to compare these methods (viz., in situ loading and physical adsorption method) regarding the loading and release behavior of payloads. In one method, gentamicin was in situ loaded on PDA-coated substrates during PDA polymerization followed by Ag NPs immobilization (named as Ag@Gen/PDA); for the second method, Ag NPs and gentamicin were simultaneously loaded onto PDA via physical adsorption by immersing pre-formed PDA coatings into a mixed solution of Ag NPs and gentamicin (named as Ag/Gen@PDA). The loading and release characteristics of these antimicrobial coatings were compared, and both gave variable outcomes. The in situ loading method consequently provided a relatively slow release of loaded antimicrobials, i.e., approx. 46% for Ag@Gen/PDA as compared to 92% from physically adsorbed Ag/GenPDA in an immersion period of 30 days. A similar trend was observed for gentamicin release, i.e., ~0.006 µg/mL from Ag@Gen/PDA and 0.02 µg/mL from Ag/Gen@PDA each day. The slower antimicrobial release from Ag@Gen/PDA coatings would ultimately provide an effective long-term antimicrobial property as compared to Ag/Gen@PDA. Finally, the synergistic antimicrobial activities of these composite coatings were assessed against two microbial species, namely, <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>, hence providing evidence in the prevention of bacterial colonization. |
| first_indexed | 2024-03-11T03:01:19Z |
| format | Article |
| id | doaj.art-1330f1506fcc45e1bd947be61feafa45 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T03:01:19Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1330f1506fcc45e1bd947be61feafa452023-11-18T08:14:10ZengMDPI AGMolecules1420-30492023-05-012811425810.3390/molecules28114258Investigation of Antimicrobial Effects of Polydopamine-Based Composite CoatingsRahila Batul0Mrinal Bhave1Aimin Yu2Department of Chemistry and Biotechnology, School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, AustraliaDepartment of Chemistry and Biotechnology, School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, AustraliaDepartment of Chemistry and Biotechnology, School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, AustraliaHerein, polydopamine (PDA)-based antimicrobial coatings loaded with silver nanoparticles (Ag NPs) and gentamicin were designed and prepared on glass slides using two different approaches. To our knowledge, this study was performed for the first time with the aim to compare these methods (viz., in situ loading and physical adsorption method) regarding the loading and release behavior of payloads. In one method, gentamicin was in situ loaded on PDA-coated substrates during PDA polymerization followed by Ag NPs immobilization (named as Ag@Gen/PDA); for the second method, Ag NPs and gentamicin were simultaneously loaded onto PDA via physical adsorption by immersing pre-formed PDA coatings into a mixed solution of Ag NPs and gentamicin (named as Ag/Gen@PDA). The loading and release characteristics of these antimicrobial coatings were compared, and both gave variable outcomes. The in situ loading method consequently provided a relatively slow release of loaded antimicrobials, i.e., approx. 46% for Ag@Gen/PDA as compared to 92% from physically adsorbed Ag/GenPDA in an immersion period of 30 days. A similar trend was observed for gentamicin release, i.e., ~0.006 µg/mL from Ag@Gen/PDA and 0.02 µg/mL from Ag/Gen@PDA each day. The slower antimicrobial release from Ag@Gen/PDA coatings would ultimately provide an effective long-term antimicrobial property as compared to Ag/Gen@PDA. Finally, the synergistic antimicrobial activities of these composite coatings were assessed against two microbial species, namely, <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>, hence providing evidence in the prevention of bacterial colonization.https://www.mdpi.com/1420-3049/28/11/4258polydopaminegentamicinsilver nanoparticlesantimicrobial coatings |
| spellingShingle | Rahila Batul Mrinal Bhave Aimin Yu Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings Molecules polydopamine gentamicin silver nanoparticles antimicrobial coatings |
| title | Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings |
| title_full | Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings |
| title_fullStr | Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings |
| title_full_unstemmed | Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings |
| title_short | Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings |
| title_sort | investigation of antimicrobial effects of polydopamine based composite coatings |
| topic | polydopamine gentamicin silver nanoparticles antimicrobial coatings |
| url | https://www.mdpi.com/1420-3049/28/11/4258 |
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