Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene
Trypanosoma evansi, an extracellular haemoprotozoan parasite, causes surra in a wide range of domestic and wild animals. In the present study, a diagnostic PCR assay was developed using primers targeting invariable surface glycoprotein (ISG) gene of T. evansi, which amplified a 196 bp product. The...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2016-06-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/59148 |
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| author | RAJENDER KUMAR DEEPAK KUMAR GAUR SACHIN KUMAR GOYAL PARVATI SHARMA SHASHI KANT KANKAR SHIKHA JAIN SANJAY KUMAR |
| author_facet | RAJENDER KUMAR DEEPAK KUMAR GAUR SACHIN KUMAR GOYAL PARVATI SHARMA SHASHI KANT KANKAR SHIKHA JAIN SANJAY KUMAR |
| author_sort | RAJENDER KUMAR |
| collection | DOAJ |
| description |
Trypanosoma evansi, an extracellular haemoprotozoan parasite, causes surra in a wide range of domestic and wild animals. In the present study, a diagnostic PCR assay was developed using primers targeting invariable surface glycoprotein (ISG) gene of T. evansi, which amplified a 196 bp product. The DNA was extracted from purified trypanosomes and T. evansi infected mice blood, and serially diluted ranging from 20 ng/µl to 0.002 pg/µl and from 90 ng/µl to 0.009 pg/µl, respectively. The diagnostic sensitivity of the PCR assay was 0.02 ng/µl (2×101 parasites) and 0.09 ng/µl (1×102 parasites) with purified parasite DNA and infected mice blood DNA, respectively. The PCR assay was also performed on extracted genomic DNA from 86 blood samples from the field out of which 3 animals were found positive by ISG-PCR assay. The developed ISG gene based PCR assay could be employed for sensitive detection of early infection, sub-clinical status of trypanosomosis and drug efficacy studies in animals.
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| first_indexed | 2024-03-12T14:25:58Z |
| format | Article |
| id | doaj.art-13327a4ad5074469b7a61ba64ddc5a25 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-12T14:25:58Z |
| publishDate | 2016-06-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-13327a4ad5074469b7a61ba64ddc5a252023-08-18T06:08:29ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272016-06-0186610.56093/ijans.v86i6.59148Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein geneRAJENDER KUMAR0DEEPAK KUMAR GAUR1SACHIN KUMAR GOYAL2PARVATI SHARMA3SHASHI KANT KANKAR4SHIKHA JAIN5SANJAY KUMAR6ICAR- National Research Centre on Equines, Hisar, Haryana 125 001 IndiaICAR- National Research Centre on Equines, Hisar, Haryana 125 001 IndiaICAR- National Research Centre on Equines, Hisar, Haryana 125 001 IndiaICAR- National Research Centre on Equines, Hisar, Haryana 125 001 IndiaCVAS, Bikaner, HisarICAR- National Research Centre on Equines, Hisar, Haryana 125 001 IndiaICAR- National Research Centre on Equines, Hisar, Haryana 125 001 India Trypanosoma evansi, an extracellular haemoprotozoan parasite, causes surra in a wide range of domestic and wild animals. In the present study, a diagnostic PCR assay was developed using primers targeting invariable surface glycoprotein (ISG) gene of T. evansi, which amplified a 196 bp product. The DNA was extracted from purified trypanosomes and T. evansi infected mice blood, and serially diluted ranging from 20 ng/µl to 0.002 pg/µl and from 90 ng/µl to 0.009 pg/µl, respectively. The diagnostic sensitivity of the PCR assay was 0.02 ng/µl (2×101 parasites) and 0.09 ng/µl (1×102 parasites) with purified parasite DNA and infected mice blood DNA, respectively. The PCR assay was also performed on extracted genomic DNA from 86 blood samples from the field out of which 3 animals were found positive by ISG-PCR assay. The developed ISG gene based PCR assay could be employed for sensitive detection of early infection, sub-clinical status of trypanosomosis and drug efficacy studies in animals. https://epubs.icar.org.in/index.php/IJAnS/article/view/59148EquinesInvariable surface glycoprotein genePCRSurraTrypanosoma evansi |
| spellingShingle | RAJENDER KUMAR DEEPAK KUMAR GAUR SACHIN KUMAR GOYAL PARVATI SHARMA SHASHI KANT KANKAR SHIKHA JAIN SANJAY KUMAR Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene Indian Journal of Animal Sciences Equines Invariable surface glycoprotein gene PCR Surra Trypanosoma evansi |
| title | Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene |
| title_full | Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene |
| title_fullStr | Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene |
| title_full_unstemmed | Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene |
| title_short | Sensitive detection of Trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene |
| title_sort | sensitive detection of trypanosoma evansi infection by polymerase chain reaction targeting invariable surface glycoprotein gene |
| topic | Equines Invariable surface glycoprotein gene PCR Surra Trypanosoma evansi |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/59148 |
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