The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen
Confocal microscope, which is a major advance upon normal light microscope, has been used in a number of scientific fields. Moreover, the three-dimensional images of specimens can be reconstructed with confocal microscope. It is ideal to analyze the three dimensional specimens for the non-destructiv...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2009-03-01
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| Series: | Nano Biomedicine and Engineering |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v1i1.p57-60 |
| _version_ | 1797673766783811584 |
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| author | Dongwu Liu Zhiwei Chen Xue Wang Hongzhi Xu Lina Wang |
| author_facet | Dongwu Liu Zhiwei Chen Xue Wang Hongzhi Xu Lina Wang |
| author_sort | Dongwu Liu |
| collection | DOAJ |
| description | Confocal microscope, which is a major advance upon normal light microscope, has been used in a number of scientific fields. Moreover, the three-dimensional images of specimens can be reconstructed with confocal microscope. It is ideal to analyze the three dimensional specimens for the non-destructive, non-invasive nature of the confocal microscope. In the present studies, a series of Weigela floridacv and Lonicera japonica thunb pollen optical sections were acquired with confocal microscope. Then the three-dimensional images of the pollen were reconstructed with the software of confocal microscope. In addition, intracellular calcium in the pollens was detected with the probe Fluo-3 AM, and the distribution of calcium in the pollens was analyzed with confocal microscope. Our results indicate that it is a very easy job to analyze the three-dimensional digital images of the pollen and intracellular calcium in the pollens with confocal microscope and the probes Acridine orange (AO) and Fluo-3 AM. |
| first_indexed | 2024-03-11T21:50:10Z |
| format | Article |
| id | doaj.art-13340e2042624d70a667786656f86665 |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2024-03-11T21:50:10Z |
| publishDate | 2009-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-13340e2042624d70a667786656f866652023-09-26T09:03:14ZengTsinghua University PressNano Biomedicine and Engineering2150-55782009-03-0111576010.5101/nbe.v1i1.p57-60The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb PollenDongwu Liu0Zhiwei Chen1Xue Wang2Hongzhi Xu3Lina Wang4Analysis and Testing Center, Shandong University of Technology, Zibo, Shandong Province 255049Analysis and Testing Center, Shandong University of Technology, Zibo, Shandong Province 255049School of Life Sciences, Shandong University of Technology, Zibo, Shandong Province 255049Analysis and Testing Center, Shandong University of Technology, Zibo, Shandong Province 255049Analysis and Testing Center, Shandong University of Technology, Zibo, Shandong Province 255049Confocal microscope, which is a major advance upon normal light microscope, has been used in a number of scientific fields. Moreover, the three-dimensional images of specimens can be reconstructed with confocal microscope. It is ideal to analyze the three dimensional specimens for the non-destructive, non-invasive nature of the confocal microscope. In the present studies, a series of Weigela floridacv and Lonicera japonica thunb pollen optical sections were acquired with confocal microscope. Then the three-dimensional images of the pollen were reconstructed with the software of confocal microscope. In addition, intracellular calcium in the pollens was detected with the probe Fluo-3 AM, and the distribution of calcium in the pollens was analyzed with confocal microscope. Our results indicate that it is a very easy job to analyze the three-dimensional digital images of the pollen and intracellular calcium in the pollens with confocal microscope and the probes Acridine orange (AO) and Fluo-3 AM.https://www.sciopen.com/article/10.5101/nbe.v1i1.p57-60confocal microscopepollenthree-dimensional imagereconstructioncalcium |
| spellingShingle | Dongwu Liu Zhiwei Chen Xue Wang Hongzhi Xu Lina Wang The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen Nano Biomedicine and Engineering confocal microscope pollen three-dimensional image reconstruction calcium |
| title | The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen |
| title_full | The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen |
| title_fullStr | The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen |
| title_full_unstemmed | The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen |
| title_short | The Three-dimensional Images and Intracellular Calcium Analysis of Weigela Floridacv and Lonicera Japonica Thunb Pollen |
| title_sort | three dimensional images and intracellular calcium analysis of weigela floridacv and lonicera japonica thunb pollen |
| topic | confocal microscope pollen three-dimensional image reconstruction calcium |
| url | https://www.sciopen.com/article/10.5101/nbe.v1i1.p57-60 |
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