The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signaling
Objectives: The objective of this study is to evaluate the effect of the total flavonoids of Clerodendrum bungei (TFCB) on the proliferation, invasion, and metastasis of A549 lung cancer cells through the Wnt signaling pathway. Materials and Methods: A549 cells were transfected with a β-catenin over...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | World Journal of Traditional Chinese Medicine |
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| Online Access: | http://www.wjtcm.net/article.asp?issn=2311-8571;year=2017;volume=3;issue=4;spage=15;epage=20;aulast=Yu |
| _version_ | 1818601040679796736 |
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| author | Na Yu Ke-Jian Zhu Si-Jing Ma Hao Tang Xiao-Ning Tan |
| author_facet | Na Yu Ke-Jian Zhu Si-Jing Ma Hao Tang Xiao-Ning Tan |
| author_sort | Na Yu |
| collection | DOAJ |
| description | Objectives: The objective of this study is to evaluate the effect of the total flavonoids of Clerodendrum bungei (TFCB) on the proliferation, invasion, and metastasis of A549 lung cancer cells through the Wnt signaling pathway. Materials and Methods: A549 cells were transfected with a β-catenin overexpression plasmid and the empty vector pcDNA3.1. The A549 cells were divided into six groups: normal A549 cell group, normal A549 cells with TFCB group, vector control group, vector with TFCB group, β-catenin overexpression group, and β-catenin with TFCB group. We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to detect cell proliferation, a scratch test was used to observe cell migration, and a transwell experiment was employed to evaluate cell invasion. Proteins related to the Wnt pathway were detected with Western blot analysis, including β-catenin, GSK-3 β, P-GSK-3 β, c-Myc, and CyclinD1. Results: The proliferation, invasion, and metastasis of A549 cells were significantly enhanced after being transfected with the β-catenin overexpression plasmid (P < 0.05 or 0.01), accompanied by increased expression of β-catenin, C-Myc, CyclinD1 and reduced expression of Gsk-3 β and P-GSK-3 β. Treatment of cells with TFCB resulted in inhibition of cell proliferation, migration, and invasion; downregulated expression of β-catenin, C-Myc, and CyclinD1; and upregulated expression of GSK-3 β and P-GSK-3 β, especially in the β-catenin overexpression group. Conclusion: TFCB has the potential to inhibit the Wnt/β-catenin pathway by prohibiting the overexpression of β-catenin and regulating its downstream factors. |
| first_indexed | 2024-12-16T12:45:04Z |
| format | Article |
| id | doaj.art-133525ac7882400c9c36b24c2f91ce48 |
| institution | Directory Open Access Journal |
| issn | 2311-8571 2589-2894 |
| language | English |
| last_indexed | 2024-12-16T12:45:04Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj.art-133525ac7882400c9c36b24c2f91ce482022-12-21T22:31:19ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712589-28942017-01-0134152010.4103/wjtcm.wjtcm_18_17The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signalingNa YuKe-Jian ZhuSi-Jing MaHao TangXiao-Ning TanObjectives: The objective of this study is to evaluate the effect of the total flavonoids of Clerodendrum bungei (TFCB) on the proliferation, invasion, and metastasis of A549 lung cancer cells through the Wnt signaling pathway. Materials and Methods: A549 cells were transfected with a β-catenin overexpression plasmid and the empty vector pcDNA3.1. The A549 cells were divided into six groups: normal A549 cell group, normal A549 cells with TFCB group, vector control group, vector with TFCB group, β-catenin overexpression group, and β-catenin with TFCB group. We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to detect cell proliferation, a scratch test was used to observe cell migration, and a transwell experiment was employed to evaluate cell invasion. Proteins related to the Wnt pathway were detected with Western blot analysis, including β-catenin, GSK-3 β, P-GSK-3 β, c-Myc, and CyclinD1. Results: The proliferation, invasion, and metastasis of A549 cells were significantly enhanced after being transfected with the β-catenin overexpression plasmid (P < 0.05 or 0.01), accompanied by increased expression of β-catenin, C-Myc, CyclinD1 and reduced expression of Gsk-3 β and P-GSK-3 β. Treatment of cells with TFCB resulted in inhibition of cell proliferation, migration, and invasion; downregulated expression of β-catenin, C-Myc, and CyclinD1; and upregulated expression of GSK-3 β and P-GSK-3 β, especially in the β-catenin overexpression group. Conclusion: TFCB has the potential to inhibit the Wnt/β-catenin pathway by prohibiting the overexpression of β-catenin and regulating its downstream factors.http://www.wjtcm.net/article.asp?issn=2311-8571;year=2017;volume=3;issue=4;spage=15;epage=20;aulast=YuMigration and invasiontotal flavonoids of Clerodendrum bungeiβ-catenin overexpression plasmidβ-catenin signaling pathway |
| spellingShingle | Na Yu Ke-Jian Zhu Si-Jing Ma Hao Tang Xiao-Ning Tan The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signaling World Journal of Traditional Chinese Medicine Migration and invasion total flavonoids of Clerodendrum bungei β-catenin overexpression plasmid β-catenin signaling pathway |
| title | The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signaling |
| title_full | The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signaling |
| title_fullStr | The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signaling |
| title_full_unstemmed | The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signaling |
| title_short | The total flavonoids of Clerodendrum bungei suppress A549 cells proliferation, migration, and invasion by impacting Wnt/β-Catenin signaling |
| title_sort | total flavonoids of clerodendrum bungei suppress a549 cells proliferation migration and invasion by impacting wnt β catenin signaling |
| topic | Migration and invasion total flavonoids of Clerodendrum bungei β-catenin overexpression plasmid β-catenin signaling pathway |
| url | http://www.wjtcm.net/article.asp?issn=2311-8571;year=2017;volume=3;issue=4;spage=15;epage=20;aulast=Yu |
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