Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics
Although <i>Vaccinium virgatum</i> Aiton leaves and stems inhibit adult T-cell leukemia (ATL) cells, leaves and stems can differ between individual plants and by time and location. In this study, leaf and stem components were profiled in the same individual plant using direct-injection e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/10/1343 |
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| author | Hisahiro Kai Yoshihito Okada Yo Goto Takayuki Nakayama Kazuhiro Sugamoto Kenjirou Ogawa Masao Yamasaki Kazuhiro Morishita Koji Matsuno Hisato Kunitake |
| author_facet | Hisahiro Kai Yoshihito Okada Yo Goto Takayuki Nakayama Kazuhiro Sugamoto Kenjirou Ogawa Masao Yamasaki Kazuhiro Morishita Koji Matsuno Hisato Kunitake |
| author_sort | Hisahiro Kai |
| collection | DOAJ |
| description | Although <i>Vaccinium virgatum</i> Aiton leaves and stems inhibit adult T-cell leukemia (ATL) cells, leaves and stems can differ between individual plants and by time and location. In this study, leaf and stem components were profiled in the same individual plant using direct-injection electron ionization-mass spectrometry (DI-EI-MS) metabolomics, with the aims of analyzing the anti-ATL activity, and quantifying proanthocyanidins (PACs). Leaves, stems, and leaf/stem mixtures showed distinct and characteristic spectra. Anti-ATL activity was stronger in stems than leaves, and the PAC content was higher in stems than leaves. These data were subjected to bivariate analysis to identify the factor (<i>m/z</i>) responsible for the inhibitory effect of ATL based on the highest coefficient of determination (<i>R</i><sup>2</sup>). The results of this DI-EI-MS metabolomics analysis suggest that among PACs contained in <i>V. virgatum</i> stems and leaves, the fragment ion at <i>m/z</i> 149 contributes significantly to anti-ATL activity. |
| first_indexed | 2024-03-10T03:02:24Z |
| format | Article |
| id | doaj.art-1335f8050bf24ed3a59dfd29eec9834b |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:02:24Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1335f8050bf24ed3a59dfd29eec9834b2023-11-23T12:42:38ZengMDPI AGPlants2223-77472022-05-011110134310.3390/plants11101343Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry MetabolomicsHisahiro Kai0Yoshihito Okada1Yo Goto2Takayuki Nakayama3Kazuhiro Sugamoto4Kenjirou Ogawa5Masao Yamasaki6Kazuhiro Morishita7Koji Matsuno8Hisato Kunitake9Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-Machi, Nobeoka, Miyazaki 882-8508, JapanDepartment of Natural Medicine and Phytochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, JapanBiolabo Co., Ltd., 7-2-6 Minatojimaminamimachi Chuo-ku, Kobe, Hyogo 650-0047, JapanBiolabo Co., Ltd., 7-2-6 Minatojimaminamimachi Chuo-ku, Kobe, Hyogo 650-0047, JapanDepartment of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, JapanOrganization for Promotion of Tenure Track, University of Miyazaki, Miyazaki 889-2192, JapanDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, JapanDepartment of Medical Sciences, Division of Tumor and Cellular Biochemistry, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, JapanDepartment of Hygiene, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka, Miyazaki 882-8508, JapanDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, JapanAlthough <i>Vaccinium virgatum</i> Aiton leaves and stems inhibit adult T-cell leukemia (ATL) cells, leaves and stems can differ between individual plants and by time and location. In this study, leaf and stem components were profiled in the same individual plant using direct-injection electron ionization-mass spectrometry (DI-EI-MS) metabolomics, with the aims of analyzing the anti-ATL activity, and quantifying proanthocyanidins (PACs). Leaves, stems, and leaf/stem mixtures showed distinct and characteristic spectra. Anti-ATL activity was stronger in stems than leaves, and the PAC content was higher in stems than leaves. These data were subjected to bivariate analysis to identify the factor (<i>m/z</i>) responsible for the inhibitory effect of ATL based on the highest coefficient of determination (<i>R</i><sup>2</sup>). The results of this DI-EI-MS metabolomics analysis suggest that among PACs contained in <i>V. virgatum</i> stems and leaves, the fragment ion at <i>m/z</i> 149 contributes significantly to anti-ATL activity.https://www.mdpi.com/2223-7747/11/10/1343metabolomicsdirect-injection electron ionization-mass spectrometry (DI-EI-MS)<i>Vaccinium virgatum</i>adult T-cell leukemia (ATL) |
| spellingShingle | Hisahiro Kai Yoshihito Okada Yo Goto Takayuki Nakayama Kazuhiro Sugamoto Kenjirou Ogawa Masao Yamasaki Kazuhiro Morishita Koji Matsuno Hisato Kunitake Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics Plants metabolomics direct-injection electron ionization-mass spectrometry (DI-EI-MS) <i>Vaccinium virgatum</i> adult T-cell leukemia (ATL) |
| title | Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics |
| title_full | Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics |
| title_fullStr | Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics |
| title_full_unstemmed | Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics |
| title_short | Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics |
| title_sort | prediction of the adult t cell leukemia inhibitory activity of blueberry leaves stems using direct injection electron ionization mass spectrometry metabolomics |
| topic | metabolomics direct-injection electron ionization-mass spectrometry (DI-EI-MS) <i>Vaccinium virgatum</i> adult T-cell leukemia (ATL) |
| url | https://www.mdpi.com/2223-7747/11/10/1343 |
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