Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.)
Leaf morphology and plant height are two agronomic traits closely related to tobacco (<i>Nicotiana tabacum</i> L.) yield and quality. The study of leaf morphology and plant stature mutants will greatly contribute to the fields of plant architecture breeding and developmental biology. Her...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/13/9/2354 |
| _version_ | 1797581623680565248 |
|---|---|
| author | Shaomei Wang Xinru Wu Yongfeng Guo Dawei Wang Lirui Cheng Yuanying Wang Aiguo Yang Guanshan Liu |
| author_facet | Shaomei Wang Xinru Wu Yongfeng Guo Dawei Wang Lirui Cheng Yuanying Wang Aiguo Yang Guanshan Liu |
| author_sort | Shaomei Wang |
| collection | DOAJ |
| description | Leaf morphology and plant height are two agronomic traits closely related to tobacco (<i>Nicotiana tabacum</i> L.) yield and quality. The study of leaf morphology and plant stature mutants will greatly contribute to the fields of plant architecture breeding and developmental biology. Here, we report the characterization of a <i>spiral leaf</i> and <i>dwarf</i> (<i>sld</i>) mutant identified from an ethylmethane sulfonate (EMS)-induced common tobacco population. The <i>sld</i> mutant displayed the phenotype of wrinkled, spiral, and miniature leaves, with the growth point as the central axis and plant dwarfing with shortened internodes. The inheritance pattern of the <i>sld</i> mutant phenotype was manipulated by a recessive nuclear monogene, which was linked to six tobacco simple sequence repeat (SSR) markers from linkage group 5 via gene mapping. Utilizing an F<sub>2</sub> population, the <i>sld</i> mutant gene the <i>sld</i> mutant gene was located between the co-segregated markers PT51778, PT54913, and the marker PT61414, with an equal genetic distance of 0.16 cM. Taking advantage of a BC<sub>1</sub>F<sub>1</sub> population, the markers PT51778, PT54913, the <i>sld</i> gene, and the marker PT61414 demonstrated co-segregation, located between the markers PT40040 and PT60933, respectively, with a genetic distance of 1.37 cM and 6.32 cM, respectively. These findings will be helpful in cloning the <i>sld</i> gene and in the further characterization of the regulatory genes controlling the spiral and dwarfing phenotypes in tobacco. |
| first_indexed | 2024-03-10T23:08:10Z |
| format | Article |
| id | doaj.art-1336c00610bf48bcad4dc324a15fb691 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T23:08:10Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-1336c00610bf48bcad4dc324a15fb6912023-11-19T09:11:06ZengMDPI AGAgronomy2073-43952023-09-01139235410.3390/agronomy13092354Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.)Shaomei Wang0Xinru Wu1Yongfeng Guo2Dawei Wang3Lirui Cheng4Yuanying Wang5Aiguo Yang6Guanshan Liu7Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaLeaf morphology and plant height are two agronomic traits closely related to tobacco (<i>Nicotiana tabacum</i> L.) yield and quality. The study of leaf morphology and plant stature mutants will greatly contribute to the fields of plant architecture breeding and developmental biology. Here, we report the characterization of a <i>spiral leaf</i> and <i>dwarf</i> (<i>sld</i>) mutant identified from an ethylmethane sulfonate (EMS)-induced common tobacco population. The <i>sld</i> mutant displayed the phenotype of wrinkled, spiral, and miniature leaves, with the growth point as the central axis and plant dwarfing with shortened internodes. The inheritance pattern of the <i>sld</i> mutant phenotype was manipulated by a recessive nuclear monogene, which was linked to six tobacco simple sequence repeat (SSR) markers from linkage group 5 via gene mapping. Utilizing an F<sub>2</sub> population, the <i>sld</i> mutant gene the <i>sld</i> mutant gene was located between the co-segregated markers PT51778, PT54913, and the marker PT61414, with an equal genetic distance of 0.16 cM. Taking advantage of a BC<sub>1</sub>F<sub>1</sub> population, the markers PT51778, PT54913, the <i>sld</i> gene, and the marker PT61414 demonstrated co-segregation, located between the markers PT40040 and PT60933, respectively, with a genetic distance of 1.37 cM and 6.32 cM, respectively. These findings will be helpful in cloning the <i>sld</i> gene and in the further characterization of the regulatory genes controlling the spiral and dwarfing phenotypes in tobacco.https://www.mdpi.com/2073-4395/13/9/2354tobaccoEMS mutagenesisspiral leaf and dwarf (<i>sld</i>) mutantSSR markersgene mapping |
| spellingShingle | Shaomei Wang Xinru Wu Yongfeng Guo Dawei Wang Lirui Cheng Yuanying Wang Aiguo Yang Guanshan Liu Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.) Agronomy tobacco EMS mutagenesis spiral leaf and dwarf (<i>sld</i>) mutant SSR markers gene mapping |
| title | Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_full | Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_fullStr | Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_full_unstemmed | Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_short | Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (<i>sld</i>) Mutant from Tetraploid Common Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_sort | phenotypic characterization and gene mapping of a spiral leaf and dwarf i sld i mutant from tetraploid common tobacco i nicotiana tabacum i l |
| topic | tobacco EMS mutagenesis spiral leaf and dwarf (<i>sld</i>) mutant SSR markers gene mapping |
| url | https://www.mdpi.com/2073-4395/13/9/2354 |
| work_keys_str_mv | AT shaomeiwang phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil AT xinruwu phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil AT yongfengguo phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil AT daweiwang phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil AT liruicheng phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil AT yuanyingwang phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil AT aiguoyang phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil AT guanshanliu phenotypiccharacterizationandgenemappingofaspiralleafanddwarfisldimutantfromtetraploidcommontobaccoinicotianatabacumil |