Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean
Abstract Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2020-11-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600302&tlng=en |
| _version_ | 1818950775303307264 |
|---|---|
| author | Talitta Regina Parmezan Salvador Lima Brito Júnior Kenia de Carvalho Moisés de Aquino Michael Birkett John Pickett Estela de Oliveira Nunes Ricardo Vilela Abdelnor Clara Beatriz Hoffmann Campo Francismar Corrêa Marcelino-Guimarães |
| author_facet | Talitta Regina Parmezan Salvador Lima Brito Júnior Kenia de Carvalho Moisés de Aquino Michael Birkett John Pickett Estela de Oliveira Nunes Ricardo Vilela Abdelnor Clara Beatriz Hoffmann Campo Francismar Corrêa Marcelino-Guimarães |
| author_sort | Talitta Regina Parmezan |
| collection | DOAJ |
| description | Abstract Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses. These VOCs originate in plants from two independent pathways: the mevalonate and the methylerythritol phosphate pathways, which utilize dimethylallyl and isopentenyl diphosphates to form the terpenoidal precursors. Phakopsora pachyrhizi fungi causes Asian soybean rust, limiting soybean production and resulting in losses of up to 80% if no control strategies are applied. By using a transcriptome datasets, we investigated the regulation of genes of the mevalonate pathway under different biotic stresses. We studied the impact of P. pachyrhizi infection in vivo expression profile of genes involved in terpenoid and glyceollin biosynthesis in genotypes harboring different resistance genes (Rpp), and across the infection cycle. In addition, we used UPLC and UPGC analysis to evaluate glyceollin and VOC production, respectively, to identify metabolites associated with soybean responses to pathogen infection. The regulation of soybean genes involved in terpene production was influenced by genotypes, depending on the Rpp gene, while glyceollin was induced in all genotypes. Furthermore, a sesquiterpene was identified as a potential marker associated with rust symptoms on soybean. |
| first_indexed | 2024-12-20T09:23:57Z |
| format | Article |
| id | doaj.art-1339346a085f4003b8a6501038e31193 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-20T09:23:57Z |
| publishDate | 2020-11-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-1339346a085f4003b8a6501038e311932022-12-21T19:45:14ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852020-11-0143410.1590/1678-4685-gmb-2019-0388Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybeanTalitta Regina ParmezanSalvador Lima Brito JúniorKenia de CarvalhoMoisés de AquinoMichael BirkettJohn PickettEstela de Oliveira NunesRicardo Vilela AbdelnorClara Beatriz Hoffmann CampoFrancismar Corrêa Marcelino-Guimarãeshttps://orcid.org/0000-0002-5828-7249Abstract Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses. These VOCs originate in plants from two independent pathways: the mevalonate and the methylerythritol phosphate pathways, which utilize dimethylallyl and isopentenyl diphosphates to form the terpenoidal precursors. Phakopsora pachyrhizi fungi causes Asian soybean rust, limiting soybean production and resulting in losses of up to 80% if no control strategies are applied. By using a transcriptome datasets, we investigated the regulation of genes of the mevalonate pathway under different biotic stresses. We studied the impact of P. pachyrhizi infection in vivo expression profile of genes involved in terpenoid and glyceollin biosynthesis in genotypes harboring different resistance genes (Rpp), and across the infection cycle. In addition, we used UPLC and UPGC analysis to evaluate glyceollin and VOC production, respectively, to identify metabolites associated with soybean responses to pathogen infection. The regulation of soybean genes involved in terpene production was influenced by genotypes, depending on the Rpp gene, while glyceollin was induced in all genotypes. Furthermore, a sesquiterpene was identified as a potential marker associated with rust symptoms on soybean.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600302&tlng=enRustGlycine maxterpenesglyceollin |
| spellingShingle | Talitta Regina Parmezan Salvador Lima Brito Júnior Kenia de Carvalho Moisés de Aquino Michael Birkett John Pickett Estela de Oliveira Nunes Ricardo Vilela Abdelnor Clara Beatriz Hoffmann Campo Francismar Corrêa Marcelino-Guimarães Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean Genetics and Molecular Biology Rust Glycine max terpenes glyceollin |
| title | Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean |
| title_full | Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean |
| title_fullStr | Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean |
| title_full_unstemmed | Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean |
| title_short | Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean |
| title_sort | transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean |
| topic | Rust Glycine max terpenes glyceollin |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600302&tlng=en |
| work_keys_str_mv | AT talittareginaparmezan transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT salvadorlimabritojunior transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT keniadecarvalho transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT moisesdeaquino transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT michaelbirkett transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT johnpickett transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT esteladeoliveiranunes transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT ricardovilelaabdelnor transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT clarabeatrizhoffmanncampo transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean AT francismarcorreamarcelinoguimaraes transcriptionalprofileofgenesinvolvedintheproductionofterpenesandglyceollinsinresponsetobioticstressesinsoybean |