The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i>
The teleost head kidney is a highly relevant immune organ, and myeloid cells play a major role in this organ’s innate and adaptive immune responses. Because of their complexity, the early phases of the innate immune reaction of fish against bacteria are still poorly understood. In this study, naïve...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/7/1/12 |
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| author | Fabio Sarais Ruth Montero Sven Ostermann Alexander Rebl Bernd Köllner Tom Goldammer |
| author_facet | Fabio Sarais Ruth Montero Sven Ostermann Alexander Rebl Bernd Köllner Tom Goldammer |
| author_sort | Fabio Sarais |
| collection | DOAJ |
| description | The teleost head kidney is a highly relevant immune organ, and myeloid cells play a major role in this organ’s innate and adaptive immune responses. Because of their complexity, the early phases of the innate immune reaction of fish against bacteria are still poorly understood. In this study, naïve rainbow trout were stimulated with inactivated <i>A. salmonicida</i> and sampled at 12 h, 24 h and 7 d poststimulation. Cells from the head kidney were magnetically sorted with a monoclonal antibody mAB21 to obtain one (MAb21-positive) fraction enriched with myeloid cells and one (MAb21-negative) fraction enriched with lymphocytes and thrombocytes. The gene expression pattern of the resulting cell subpopulations was analysed using a panel of 43 immune-related genes. The results show an overall downregulation of the complement pathway and cytokine production at the considered time points. Some of the selected genes may be considered as parameters for diagnosing bacterial furunculosis of rainbow trout. |
| first_indexed | 2024-03-09T21:59:16Z |
| format | Article |
| id | doaj.art-133a25bddb034568b608829905f53f89 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-09T21:59:16Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-133a25bddb034568b608829905f53f892023-11-23T19:51:22ZengMDPI AGFishes2410-38882022-01-01711210.3390/fishes7010012The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i>Fabio Sarais0Ruth Montero1Sven Ostermann2Alexander Rebl3Bernd Köllner4Tom Goldammer5Research Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, GermanyFriedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Immunology, 17493 Greifswald, GermanyFriedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Immunology, 17493 Greifswald, GermanyResearch Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, GermanyFriedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Immunology, 17493 Greifswald, GermanyResearch Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, GermanyThe teleost head kidney is a highly relevant immune organ, and myeloid cells play a major role in this organ’s innate and adaptive immune responses. Because of their complexity, the early phases of the innate immune reaction of fish against bacteria are still poorly understood. In this study, naïve rainbow trout were stimulated with inactivated <i>A. salmonicida</i> and sampled at 12 h, 24 h and 7 d poststimulation. Cells from the head kidney were magnetically sorted with a monoclonal antibody mAB21 to obtain one (MAb21-positive) fraction enriched with myeloid cells and one (MAb21-negative) fraction enriched with lymphocytes and thrombocytes. The gene expression pattern of the resulting cell subpopulations was analysed using a panel of 43 immune-related genes. The results show an overall downregulation of the complement pathway and cytokine production at the considered time points. Some of the selected genes may be considered as parameters for diagnosing bacterial furunculosis of rainbow trout.https://www.mdpi.com/2410-3888/7/1/12<i>Aeromonas</i>NF-κBmyeloidinflammatoryinnate immune responserainbow trout |
| spellingShingle | Fabio Sarais Ruth Montero Sven Ostermann Alexander Rebl Bernd Köllner Tom Goldammer The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i> Fishes <i>Aeromonas</i> NF-κB myeloid inflammatory innate immune response rainbow trout |
| title | The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i> |
| title_full | The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i> |
| title_fullStr | The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i> |
| title_full_unstemmed | The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i> |
| title_short | The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Stimulated with <i>Aeromonas salmonicida</i> |
| title_sort | early immune response of lymphoid and myeloid head kidney cells of rainbow trout i oncorhynchus mykiss i stimulated with i aeromonas salmonicida i |
| topic | <i>Aeromonas</i> NF-κB myeloid inflammatory innate immune response rainbow trout |
| url | https://www.mdpi.com/2410-3888/7/1/12 |
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