Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide
Pseudoalteromonas is a ubiquitous and abundant genus of marine bacteria commonly associated with algae. In this study, a novel siphoviral-morphological bacteriophage, vB_PhoS_XC, was isolated from the coastal seawaters of Qingdao (China) during a bloom of the Ulva prolifera (U. prolifera) green tide...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1201434/full |
| _version_ | 1797777759652544512 |
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| author | Jianhua Sun Xinran Zhang Yantao Liang Yantao Liang Kaiyang Zheng Fraser Kennedy Meiaoxue Han Gang Liu Yundan Liu Ziyue Wang Xuechao Chen Yeong Yik Sung Yeong Yik Sung Wen Jye Mok Wen Jye Mok Li Lian Wong Li Lian Wong Andrew McMinn Andrew McMinn Min Wang Min Wang Min Wang Min Wang |
| author_facet | Jianhua Sun Xinran Zhang Yantao Liang Yantao Liang Kaiyang Zheng Fraser Kennedy Meiaoxue Han Gang Liu Yundan Liu Ziyue Wang Xuechao Chen Yeong Yik Sung Yeong Yik Sung Wen Jye Mok Wen Jye Mok Li Lian Wong Li Lian Wong Andrew McMinn Andrew McMinn Min Wang Min Wang Min Wang Min Wang |
| author_sort | Jianhua Sun |
| collection | DOAJ |
| description | Pseudoalteromonas is a ubiquitous and abundant genus of marine bacteria commonly associated with algae. In this study, a novel siphoviral-morphological bacteriophage, vB_PhoS_XC, was isolated from the coastal seawaters of Qingdao (China) during a bloom of the Ulva prolifera (U. prolifera) green tide. The morphology of this phage (icosahedron head 51 ± 1 nm in diameter; a tail length of 86 ± 1 nm) was characterized through transmission electron microscope. The biological properties of this virus showed a short latent period (45 minutes), a large burst size (241 virions per cell) and a relatively wide range of temperatures/pH level tolerance (-20°C to 45°C and pH 4 to pH 10, respectively). The vB_PhoS_XC has a 46,490-bp double-stranded DNA genome with a G+C content of 40.0%, and encodes 72 open reading frames (ORFs). Thirty-five of these ORFs were assigned into known functions based on BLAST-based algorithm against NR database of GenBank. In addition, eco-genomic analysis provides the evidence of vB_PhoS_XC accompanied by bloom of U. prolifera, and confirmed the high expression of two phosphatase-metabolism-related auxiliary metabolic genes (AMGs). This study provides new insights into the functional and ecological roles of the Pseudoalteromonas phage vB_PhoS_XC, shedding light on the virological study approach combined with traditional isolation and meta-omics data. |
| first_indexed | 2024-03-12T23:09:16Z |
| format | Article |
| id | doaj.art-133fbf5572b644b69854e678fbd4afae |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-12T23:09:16Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-133fbf5572b644b69854e678fbd4afae2023-07-18T08:18:00ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-07-011010.3389/fmars.2023.12014341201434Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tideJianhua Sun0Xinran Zhang1Yantao Liang2Yantao Liang3Kaiyang Zheng4Fraser Kennedy5Meiaoxue Han6Gang Liu7Yundan Liu8Ziyue Wang9Xuechao Chen10Yeong Yik Sung11Yeong Yik Sung12Wen Jye Mok13Wen Jye Mok14Li Lian Wong15Li Lian Wong16Andrew McMinn17Andrew McMinn18Min Wang19Min Wang20Min Wang21Min Wang22College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaUniversiti Malaysia Terengganu (UMT) - Ocean University of China (OUC) Joint Centre for Marine Studies, Qingdao, ChinaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaInstitute for Marine and Antarctic Studies, University of Tasmania, Hobart, TAS, AustraliaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaUniversiti Malaysia Terengganu (UMT) - Ocean University of China (OUC) Joint Centre for Marine Studies, Qingdao, ChinaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu (UMT), Kuala Nerus, MalaysiaUniversiti Malaysia Terengganu (UMT) - Ocean University of China (OUC) Joint Centre for Marine Studies, Qingdao, ChinaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu (UMT), Kuala Nerus, MalaysiaUniversiti Malaysia Terengganu (UMT) - Ocean University of China (OUC) Joint Centre for Marine Studies, Qingdao, ChinaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu (UMT), Kuala Nerus, MalaysiaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaInstitute for Marine and Antarctic Studies, University of Tasmania, Hobart, TAS, AustraliaCollege of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, and Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, ChinaUniversiti Malaysia Terengganu (UMT) - Ocean University of China (OUC) Joint Centre for Marine Studies, Qingdao, ChinaHaide College, Ocean University of China, Qingdao, ChinaThe Affiliated Hospital of Qingdao University, Qingdao, ChinaPseudoalteromonas is a ubiquitous and abundant genus of marine bacteria commonly associated with algae. In this study, a novel siphoviral-morphological bacteriophage, vB_PhoS_XC, was isolated from the coastal seawaters of Qingdao (China) during a bloom of the Ulva prolifera (U. prolifera) green tide. The morphology of this phage (icosahedron head 51 ± 1 nm in diameter; a tail length of 86 ± 1 nm) was characterized through transmission electron microscope. The biological properties of this virus showed a short latent period (45 minutes), a large burst size (241 virions per cell) and a relatively wide range of temperatures/pH level tolerance (-20°C to 45°C and pH 4 to pH 10, respectively). The vB_PhoS_XC has a 46,490-bp double-stranded DNA genome with a G+C content of 40.0%, and encodes 72 open reading frames (ORFs). Thirty-five of these ORFs were assigned into known functions based on BLAST-based algorithm against NR database of GenBank. In addition, eco-genomic analysis provides the evidence of vB_PhoS_XC accompanied by bloom of U. prolifera, and confirmed the high expression of two phosphatase-metabolism-related auxiliary metabolic genes (AMGs). This study provides new insights into the functional and ecological roles of the Pseudoalteromonas phage vB_PhoS_XC, shedding light on the virological study approach combined with traditional isolation and meta-omics data.https://www.frontiersin.org/articles/10.3389/fmars.2023.1201434/fullbacteriophagePseudoalteromonasUlva prolifera bloomsgenomic and meta-omics analysisecological footprint |
| spellingShingle | Jianhua Sun Xinran Zhang Yantao Liang Yantao Liang Kaiyang Zheng Fraser Kennedy Meiaoxue Han Gang Liu Yundan Liu Ziyue Wang Xuechao Chen Yeong Yik Sung Yeong Yik Sung Wen Jye Mok Wen Jye Mok Li Lian Wong Li Lian Wong Andrew McMinn Andrew McMinn Min Wang Min Wang Min Wang Min Wang Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide Frontiers in Marine Science bacteriophage Pseudoalteromonas Ulva prolifera blooms genomic and meta-omics analysis ecological footprint |
| title | Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide |
| title_full | Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide |
| title_fullStr | Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide |
| title_full_unstemmed | Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide |
| title_short | Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide |
| title_sort | abundance and ecological footprint of pseudoalteromonas phage vb phos xc in the ulva prolifera green tide |
| topic | bacteriophage Pseudoalteromonas Ulva prolifera blooms genomic and meta-omics analysis ecological footprint |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1201434/full |
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