The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation
The enhancement of ultraviolet-B radiation (UV-B) radiation reaching the Earth’s surface due to ozone layer depletion is an important topic. Macroalgal species growing in the intertidal zone are often directly exposed to UV-B radiation periodically as the tide changes. In order to better understand...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.904943/full |
| _version_ | 1828171186902663168 |
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| author | Yan Sun Yan Sun Yu Zang Yu Zang Jun Chen Jun Chen Shuai Shang Jing Wang Jing Wang Qian Liu Qian Liu Xuexi Tang Xuexi Tang |
| author_facet | Yan Sun Yan Sun Yu Zang Yu Zang Jun Chen Jun Chen Shuai Shang Jing Wang Jing Wang Qian Liu Qian Liu Xuexi Tang Xuexi Tang |
| author_sort | Yan Sun |
| collection | DOAJ |
| description | The enhancement of ultraviolet-B radiation (UV-B) radiation reaching the Earth’s surface due to ozone layer depletion is an important topic. Macroalgal species growing in the intertidal zone are often directly exposed to UV-B radiation periodically as the tide changes. In order to better understand the response of macroalgae to UV-B stressed condition, we studied the dominant dioecious intertidal macroalgae Sargassum thunbergii. After consecutive UV-B radiation treatments, we used metabonomics models to analyze and compare the maximum photosynthetic electron transport rate (ETRmax), central carbon cycle metabolism (CCCM) gene expression level, CCCM enzymic activities [pyruvate dehydrogenase and citrate synthase (PDH and CS)], and carbon-based metabolite (including pyruvate, soluble sugar, total amino acid, and lipids) content in male and female S. thunbergii. The results showed that under low and high UV-B radiation, the ETRmax values and six targeted CCCM gene expression levels were significantly higher in males than in females. Under high UV-B radiation, only the CS activity was significantly higher in males than in females. There was no significant difference in PDH activity between males and females. The CCCM models constructed using the metabonomics analysis demonstrate that S. thunbergii males and females exhibit obvious gender differences in their responses to UV-B radiation, providing us with a new understanding of the macroalgal gender differences under UV-B radiation, as past investigations always underestimated their diecious characteristics. |
| first_indexed | 2024-04-12T03:24:42Z |
| format | Article |
| id | doaj.art-133ff741780a4c6cb5fcf5ba527b2c6d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T03:24:42Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-133ff741780a4c6cb5fcf5ba527b2c6d2022-12-22T03:49:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.904943904943The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiationYan Sun0Yan Sun1Yu Zang2Yu Zang3Jun Chen4Jun Chen5Shuai Shang6Jing Wang7Jing Wang8Qian Liu9Qian Liu10Xuexi Tang11Xuexi Tang12College of Marine Life Sciences, Ocean University of China, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCollege of Biological and Environmental Engineering, Binzhou University, Binzhou, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaThe enhancement of ultraviolet-B radiation (UV-B) radiation reaching the Earth’s surface due to ozone layer depletion is an important topic. Macroalgal species growing in the intertidal zone are often directly exposed to UV-B radiation periodically as the tide changes. In order to better understand the response of macroalgae to UV-B stressed condition, we studied the dominant dioecious intertidal macroalgae Sargassum thunbergii. After consecutive UV-B radiation treatments, we used metabonomics models to analyze and compare the maximum photosynthetic electron transport rate (ETRmax), central carbon cycle metabolism (CCCM) gene expression level, CCCM enzymic activities [pyruvate dehydrogenase and citrate synthase (PDH and CS)], and carbon-based metabolite (including pyruvate, soluble sugar, total amino acid, and lipids) content in male and female S. thunbergii. The results showed that under low and high UV-B radiation, the ETRmax values and six targeted CCCM gene expression levels were significantly higher in males than in females. Under high UV-B radiation, only the CS activity was significantly higher in males than in females. There was no significant difference in PDH activity between males and females. The CCCM models constructed using the metabonomics analysis demonstrate that S. thunbergii males and females exhibit obvious gender differences in their responses to UV-B radiation, providing us with a new understanding of the macroalgal gender differences under UV-B radiation, as past investigations always underestimated their diecious characteristics.https://www.frontiersin.org/articles/10.3389/fpls.2022.904943/fullSargassum thunbergiicentral carbon cycle metabolismmetabolitesdiecious differencesUV-B radiation |
| spellingShingle | Yan Sun Yan Sun Yu Zang Yu Zang Jun Chen Jun Chen Shuai Shang Jing Wang Jing Wang Qian Liu Qian Liu Xuexi Tang Xuexi Tang The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation Frontiers in Plant Science Sargassum thunbergii central carbon cycle metabolism metabolites diecious differences UV-B radiation |
| title | The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation |
| title_full | The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation |
| title_fullStr | The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation |
| title_full_unstemmed | The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation |
| title_short | The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation |
| title_sort | differing responses of central carbon cycle metabolism in male and female sargassum thunbergii to ultraviolet b radiation |
| topic | Sargassum thunbergii central carbon cycle metabolism metabolites diecious differences UV-B radiation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.904943/full |
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