Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model.
Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bac...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3102678?pdf=render |
| _version_ | 1818315648520945664 |
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| author | Abraham Lin Jose Jimenez Julien Derr Pedro Vera Michael L Manapat Kevin M Esvelt Laura Villanueva David R Liu Irene A Chen |
| author_facet | Abraham Lin Jose Jimenez Julien Derr Pedro Vera Michael L Manapat Kevin M Esvelt Laura Villanueva David R Liu Irene A Chen |
| author_sort | Abraham Lin |
| collection | DOAJ |
| description | Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit conjugation several decades ago. Here we investigate the mechanism of inhibition and find that the primary effect on conjugation is occlusion of the conjugative pilus by phage particles. This interaction is mediated primarily by phage coat protein g3p, and exogenous addition of the soluble fragment of g3p inhibited conjugation at low nanomolar concentrations. Our data are quantitatively consistent with a simple model in which association between the pili and phage particles or g3p prevents transmission of an F plasmid encoding tetracycline resistance. We also observe a decrease in the donor ability of infected cells, which is quantitatively consistent with a reduction in pili elaboration. Since many antibiotic-resistance factors confer susceptibility to phage infection through expression of conjugative pili (the receptor for filamentous phage), these results suggest that phage may be a source of soluble proteins that slow the spread of antibiotic resistance genes. |
| first_indexed | 2024-12-13T09:08:53Z |
| format | Article |
| id | doaj.art-134051446cda496692a61af0f740d0bc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T09:08:53Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-134051446cda496692a61af0f740d0bc2022-12-21T23:53:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e1999110.1371/journal.pone.0019991Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model.Abraham LinJose JimenezJulien DerrPedro VeraMichael L ManapatKevin M EsveltLaura VillanuevaDavid R LiuIrene A ChenConjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit conjugation several decades ago. Here we investigate the mechanism of inhibition and find that the primary effect on conjugation is occlusion of the conjugative pilus by phage particles. This interaction is mediated primarily by phage coat protein g3p, and exogenous addition of the soluble fragment of g3p inhibited conjugation at low nanomolar concentrations. Our data are quantitatively consistent with a simple model in which association between the pili and phage particles or g3p prevents transmission of an F plasmid encoding tetracycline resistance. We also observe a decrease in the donor ability of infected cells, which is quantitatively consistent with a reduction in pili elaboration. Since many antibiotic-resistance factors confer susceptibility to phage infection through expression of conjugative pili (the receptor for filamentous phage), these results suggest that phage may be a source of soluble proteins that slow the spread of antibiotic resistance genes.http://europepmc.org/articles/PMC3102678?pdf=render |
| spellingShingle | Abraham Lin Jose Jimenez Julien Derr Pedro Vera Michael L Manapat Kevin M Esvelt Laura Villanueva David R Liu Irene A Chen Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. PLoS ONE |
| title | Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. |
| title_full | Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. |
| title_fullStr | Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. |
| title_full_unstemmed | Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. |
| title_short | Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. |
| title_sort | inhibition of bacterial conjugation by phage m13 and its protein g3p quantitative analysis and model |
| url | http://europepmc.org/articles/PMC3102678?pdf=render |
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