A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans
Abstract XRN2 is an evolutionarily conserved 5’-to-3’ exoribonuclease, which degrades or trims various types of RNA in the nucleus. Although XRN-2 is essential for embryogenesis, larval development and reproduction in Caenorhabditis elegans, relevant molecular pathways remain unidentified. Here we c...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2023-05-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572023000400701&lng=en&tlng=en |
| _version_ | 1797826193309827072 |
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| author | Ilkin Aygün Alicja Rzepczak Takashi S. Miki |
| author_facet | Ilkin Aygün Alicja Rzepczak Takashi S. Miki |
| author_sort | Ilkin Aygün |
| collection | DOAJ |
| description | Abstract XRN2 is an evolutionarily conserved 5’-to-3’ exoribonuclease, which degrades or trims various types of RNA in the nucleus. Although XRN-2 is essential for embryogenesis, larval development and reproduction in Caenorhabditis elegans, relevant molecular pathways remain unidentified. Here we create a germline-specific xrn-2 conditional mutant and perform a mutagenesis screen for suppressors of sterility. Loss-of-function alleles of dpy-10, osr-1, ptr-6 and C34C12.2 genes are identified. Depletion of DPY-10, OSR-1 or PTR-6 increases expression of gpdh-1 that encodes a glycerol-3-phosphate dehydrogenase, thereby elevates glycerol accumulation to suppress sterility of the mutant. The C34C12.2 protein is predominantly localized in the nucleolus of germ cells and shows a similarity to Saccharomyces cerevisiae Net1, which is involved in rDNA silencing. Depletion of NRDE-2, a putative interacting partner of C34C12.2 and a component of the nuclear RNAi machinery, restores fertility to the xrn-2 conditional mutant. These results may help to identify an essential role of XRN-2 in germline development. |
| first_indexed | 2024-03-13T11:05:06Z |
| format | Article |
| id | doaj.art-13417481bd09435aa65e2f15315b7034 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-03-13T11:05:06Z |
| publishDate | 2023-05-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-13417481bd09435aa65e2f15315b70342023-05-16T07:35:37ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852023-05-0146210.1590/1678-4685-gmb-2022-0328A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegansIlkin AygünAlicja RzepczakTakashi S. Mikihttps://orcid.org/0000-0003-3715-7039Abstract XRN2 is an evolutionarily conserved 5’-to-3’ exoribonuclease, which degrades or trims various types of RNA in the nucleus. Although XRN-2 is essential for embryogenesis, larval development and reproduction in Caenorhabditis elegans, relevant molecular pathways remain unidentified. Here we create a germline-specific xrn-2 conditional mutant and perform a mutagenesis screen for suppressors of sterility. Loss-of-function alleles of dpy-10, osr-1, ptr-6 and C34C12.2 genes are identified. Depletion of DPY-10, OSR-1 or PTR-6 increases expression of gpdh-1 that encodes a glycerol-3-phosphate dehydrogenase, thereby elevates glycerol accumulation to suppress sterility of the mutant. The C34C12.2 protein is predominantly localized in the nucleolus of germ cells and shows a similarity to Saccharomyces cerevisiae Net1, which is involved in rDNA silencing. Depletion of NRDE-2, a putative interacting partner of C34C12.2 and a component of the nuclear RNAi machinery, restores fertility to the xrn-2 conditional mutant. These results may help to identify an essential role of XRN-2 in germline development.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572023000400701&lng=en&tlng=enXRN2C34C12.2glycerol regulationmutagenesis screengermline development |
| spellingShingle | Ilkin Aygün Alicja Rzepczak Takashi S. Miki A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans Genetics and Molecular Biology XRN2 C34C12.2 glycerol regulation mutagenesis screen germline development |
| title | A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans |
| title_full | A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans |
| title_fullStr | A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans |
| title_full_unstemmed | A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans |
| title_short | A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans |
| title_sort | germline targeted genetic screen for xrn 2 suppressors identifies a novel gene c34c12 2 in caenorhabditis elegans |
| topic | XRN2 C34C12.2 glycerol regulation mutagenesis screen germline development |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572023000400701&lng=en&tlng=en |
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