Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i>
Manganese superoxide dismutases (MnSODs) play a pivotal role in the preservation of mitochondrial integrity and function in fungi under various endogenous and exogenous stresses. Deletion of <i>Aspergillus nidulans</i> mnSOD/SodB increased oxidative stress sensitivity and apoptotic cell...
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MDPI AG
2023-10-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/11/1060 |
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| author | Klaudia Pákozdi Tamás Emri Károly Antal István Pócsi |
| author_facet | Klaudia Pákozdi Tamás Emri Károly Antal István Pócsi |
| author_sort | Klaudia Pákozdi |
| collection | DOAJ |
| description | Manganese superoxide dismutases (MnSODs) play a pivotal role in the preservation of mitochondrial integrity and function in fungi under various endogenous and exogenous stresses. Deletion of <i>Aspergillus nidulans</i> mnSOD/SodB increased oxidative stress sensitivity and apoptotic cell death rates as well as affected antioxidant enzyme and sterigmatocystin productions, respiration, conidiation and the stress tolerance of conidiospores. The physiological consequences of the lack of <i>sodB</i> were more pronounced during carbon starvation than in the presence of glucose. Lack of SodB also affected the changes in the transcriptome, recorded by high-throughput RNA sequencing, in menadione sodium bisulfite (MSB)-exposed, submerged cultures supplemented with glucose. Surprisingly, the difference between the global transcriptional changes of the <i>ΔsodB</i> mutant and the control strain were relatively small, indicating that the SodB-dependent maintenance of mitochondrial integrity was not essential under these experimental conditions. Owing to the outstanding physiological flexibility of the Aspergilli, certain antioxidant enzymes and endogenous antioxidants together with the reduction in mitochondrial functions compensated well for the lack of SodB. The lack of <i>sodB</i> reduced the growth of surface cultures more than of the submerged culture, which should be considered in future development of fungal disinfection methods. |
| first_indexed | 2024-03-09T16:41:43Z |
| format | Article |
| id | doaj.art-134176d1056d4916a8a432f1d682a9d8 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T16:41:43Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-134176d1056d4916a8a432f1d682a9d82023-11-24T14:50:59ZengMDPI AGJournal of Fungi2309-608X2023-10-01911106010.3390/jof9111060Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i>Klaudia Pákozdi0Tamás Emri1Károly Antal2István Pócsi3Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Zoology, Eszterházy Károly Catholic University, H-3300 Eger, HungaryDepartment of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryManganese superoxide dismutases (MnSODs) play a pivotal role in the preservation of mitochondrial integrity and function in fungi under various endogenous and exogenous stresses. Deletion of <i>Aspergillus nidulans</i> mnSOD/SodB increased oxidative stress sensitivity and apoptotic cell death rates as well as affected antioxidant enzyme and sterigmatocystin productions, respiration, conidiation and the stress tolerance of conidiospores. The physiological consequences of the lack of <i>sodB</i> were more pronounced during carbon starvation than in the presence of glucose. Lack of SodB also affected the changes in the transcriptome, recorded by high-throughput RNA sequencing, in menadione sodium bisulfite (MSB)-exposed, submerged cultures supplemented with glucose. Surprisingly, the difference between the global transcriptional changes of the <i>ΔsodB</i> mutant and the control strain were relatively small, indicating that the SodB-dependent maintenance of mitochondrial integrity was not essential under these experimental conditions. Owing to the outstanding physiological flexibility of the Aspergilli, certain antioxidant enzymes and endogenous antioxidants together with the reduction in mitochondrial functions compensated well for the lack of SodB. The lack of <i>sodB</i> reduced the growth of surface cultures more than of the submerged culture, which should be considered in future development of fungal disinfection methods.https://www.mdpi.com/2309-608X/9/11/1060<i>Aspergillus nidulans</i>menadione stressmitochondrial superoxide dismutaseRNA sequencingSodB |
| spellingShingle | Klaudia Pákozdi Tamás Emri Károly Antal István Pócsi Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i> Journal of Fungi <i>Aspergillus nidulans</i> menadione stress mitochondrial superoxide dismutase RNA sequencing SodB |
| title | Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i> |
| title_full | Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i> |
| title_fullStr | Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i> |
| title_full_unstemmed | Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i> |
| title_short | Global Transcriptomic Changes Elicited by <i>sodB</i> Deletion and Menadione Exposure in <i>Aspergillus nidulans</i> |
| title_sort | global transcriptomic changes elicited by i sodb i deletion and menadione exposure in i aspergillus nidulans i |
| topic | <i>Aspergillus nidulans</i> menadione stress mitochondrial superoxide dismutase RNA sequencing SodB |
| url | https://www.mdpi.com/2309-608X/9/11/1060 |
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