A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin
Microfluidic paper-based analytical devices (µPADs) represent one of the promising green analytical strategies for low-cost and simple determination of various analytes. The actual task is the development of such devices for quantitation of antioxidants, e.g., flavonoids. In this paper, possibilitie...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/22/8/2893 |
| _version_ | 1797434201424789504 |
|---|---|
| author | Vladimir V. Apyari Aleksei A. Furletov Vyacheslav I. Kalinin Stanislava G. Dmitrienko Yury A. Zolotov |
| author_facet | Vladimir V. Apyari Aleksei A. Furletov Vyacheslav I. Kalinin Stanislava G. Dmitrienko Yury A. Zolotov |
| author_sort | Vladimir V. Apyari |
| collection | DOAJ |
| description | Microfluidic paper-based analytical devices (µPADs) represent one of the promising green analytical strategies for low-cost and simple determination of various analytes. The actual task is the development of such devices for quantitation of antioxidants, e.g., flavonoids. In this paper, possibilities of a novel three-reagent µPAD including silver nitrate, 4-nitrophenyldiazonium tetrafluoroborate, and iron(III) chloride as reagents are assessed with respect to the determination of dihydroquercetin. It is shown that all the three reagents produce different colorimetric responses that can be detected by a mini-spectrophotometer–monitor calibrator or by a smartphone. The method is applicable to direct measuring high contents of dihydroquercetin (the linearity range is 0.026–1 mg mL<sup>−1</sup>, and the limit of detection is 7.7 µg mL<sup>−1</sup>), which is favorable for many dietary supplements. The analysis of a food supplement was possible with the relative standard deviations of 9–26%, which is satisfactory for quantitative and semiquantitative determinations. It was found that plotting a calibration graph in 3D space of the three reagents’ responses allows us to distinguish dihydroquercetin from its close structural analogue, quercetin. |
| first_indexed | 2024-03-09T10:29:47Z |
| format | Article |
| id | doaj.art-1347ebdf47774ab9af17840aa2b1ab10 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T10:29:47Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1347ebdf47774ab9af17840aa2b1ab102023-12-01T21:22:48ZengMDPI AGSensors1424-82202022-04-01228289310.3390/s22082893A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of DihydroquercetinVladimir V. Apyari0Aleksei A. Furletov1Vyacheslav I. Kalinin2Stanislava G. Dmitrienko3Yury A. Zolotov4Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, 119991 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, 119991 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, 119991 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, 119991 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, 119991 Moscow, RussiaMicrofluidic paper-based analytical devices (µPADs) represent one of the promising green analytical strategies for low-cost and simple determination of various analytes. The actual task is the development of such devices for quantitation of antioxidants, e.g., flavonoids. In this paper, possibilities of a novel three-reagent µPAD including silver nitrate, 4-nitrophenyldiazonium tetrafluoroborate, and iron(III) chloride as reagents are assessed with respect to the determination of dihydroquercetin. It is shown that all the three reagents produce different colorimetric responses that can be detected by a mini-spectrophotometer–monitor calibrator or by a smartphone. The method is applicable to direct measuring high contents of dihydroquercetin (the linearity range is 0.026–1 mg mL<sup>−1</sup>, and the limit of detection is 7.7 µg mL<sup>−1</sup>), which is favorable for many dietary supplements. The analysis of a food supplement was possible with the relative standard deviations of 9–26%, which is satisfactory for quantitative and semiquantitative determinations. It was found that plotting a calibration graph in 3D space of the three reagents’ responses allows us to distinguish dihydroquercetin from its close structural analogue, quercetin.https://www.mdpi.com/1424-8220/22/8/2893microfluidic paper-based analytical devicesantioxidantsflavonoidsdihydroquercetinoptical sensorssolid-phase spectrophotometry |
| spellingShingle | Vladimir V. Apyari Aleksei A. Furletov Vyacheslav I. Kalinin Stanislava G. Dmitrienko Yury A. Zolotov A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin Sensors microfluidic paper-based analytical devices antioxidants flavonoids dihydroquercetin optical sensors solid-phase spectrophotometry |
| title | A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin |
| title_full | A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin |
| title_fullStr | A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin |
| title_full_unstemmed | A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin |
| title_short | A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin |
| title_sort | three reagent green paper based analytical device for solid phase spectrometric and colorimetric determination of dihydroquercetin |
| topic | microfluidic paper-based analytical devices antioxidants flavonoids dihydroquercetin optical sensors solid-phase spectrophotometry |
| url | https://www.mdpi.com/1424-8220/22/8/2893 |
| work_keys_str_mv | AT vladimirvapyari athreereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT alekseiafurletov athreereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT vyacheslavikalinin athreereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT stanislavagdmitrienko athreereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT yuryazolotov athreereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT vladimirvapyari threereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT alekseiafurletov threereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT vyacheslavikalinin threereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT stanislavagdmitrienko threereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin AT yuryazolotov threereagentgreenpaperbasedanalyticaldeviceforsolidphasespectrometricandcolorimetricdeterminationofdihydroquercetin |