Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid production
Abstract The recent scarcity of fishmeal and other resources means that studies on the intrinsic mechanisms of nutrients in the growth and development of aquatic animals at the molecular level have received widespread attention. The target of rapamycin (TOR) pathway has been reported to receive sign...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-50300-3 |
| _version_ | 1797276521277161472 |
|---|---|
| author | Xilin Dai Xuenan Li Danhui Yin Xin Chen Linwei Wang Luyao Pang Yuanshuai Fu |
| author_facet | Xilin Dai Xuenan Li Danhui Yin Xin Chen Linwei Wang Luyao Pang Yuanshuai Fu |
| author_sort | Xilin Dai |
| collection | DOAJ |
| description | Abstract The recent scarcity of fishmeal and other resources means that studies on the intrinsic mechanisms of nutrients in the growth and development of aquatic animals at the molecular level have received widespread attention. The target of rapamycin (TOR) pathway has been reported to receive signals from nutrients and environmental stresses, and regulates cellular anabolism and catabolism to achieve precise regulation of cell growth and physiological activities. In this study, we cloned and characterized the full-length cDNA sequence of the TOR gene of Macrobrachium rosenbergii (MrTOR). MrTOR was expressed in all tissues, with higher expression in heart and muscle tissues. In situ hybridization also indicated that MrTOR was expressed in muscle, mainly around the nucleus. RNA interference decreased the expression levels of MrTOR and downstream protein synthesis-related genes (S6K, eIF4E, and eIF4B) (P < 0.05) and the expression and enzyme activity of the lipid synthesis-related enzyme, fatty acid synthase (FAS), and increased enzyme activity of the lipolysis-related enzyme, lipase (LPS). In addition, amino acid injection significantly increased the transcript levels of MrTOR and downstream related genes (S6K, eIF4E, eIF4B, and FAS), as well as triglyceride and total cholesterol tissue levels and FAS activity. Starvation significantly increased transcript levels and enzyme activities of adenylate-activated protein kinase and LPS and decreased transcript levels and enzyme activities of FAS, as well as transcript levels of MrTOR and its downstream genes (P < 0.05), whereas amino acid injection alleviated the starvation-induced decreases in transcript levels of these genes. These results suggested that arginine and leucine activated the TOR signaling pathway, promoted protein and lipid syntheses, and alleviated the pathway changes induced by starvation. |
| first_indexed | 2024-03-07T15:29:22Z |
| format | Article |
| id | doaj.art-1349a0e05cb04cdb888f2066bbd16115 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:29:22Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1349a0e05cb04cdb888f2066bbd161152024-03-05T16:28:50ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-023-50300-3Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid productionXilin Dai0Xuenan Li1Danhui Yin2Xin Chen3Linwei Wang4Luyao Pang5Yuanshuai Fu6Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean UniversityKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean UniversityKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean UniversityKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean UniversityKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean UniversityKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean UniversityKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean UniversityAbstract The recent scarcity of fishmeal and other resources means that studies on the intrinsic mechanisms of nutrients in the growth and development of aquatic animals at the molecular level have received widespread attention. The target of rapamycin (TOR) pathway has been reported to receive signals from nutrients and environmental stresses, and regulates cellular anabolism and catabolism to achieve precise regulation of cell growth and physiological activities. In this study, we cloned and characterized the full-length cDNA sequence of the TOR gene of Macrobrachium rosenbergii (MrTOR). MrTOR was expressed in all tissues, with higher expression in heart and muscle tissues. In situ hybridization also indicated that MrTOR was expressed in muscle, mainly around the nucleus. RNA interference decreased the expression levels of MrTOR and downstream protein synthesis-related genes (S6K, eIF4E, and eIF4B) (P < 0.05) and the expression and enzyme activity of the lipid synthesis-related enzyme, fatty acid synthase (FAS), and increased enzyme activity of the lipolysis-related enzyme, lipase (LPS). In addition, amino acid injection significantly increased the transcript levels of MrTOR and downstream related genes (S6K, eIF4E, eIF4B, and FAS), as well as triglyceride and total cholesterol tissue levels and FAS activity. Starvation significantly increased transcript levels and enzyme activities of adenylate-activated protein kinase and LPS and decreased transcript levels and enzyme activities of FAS, as well as transcript levels of MrTOR and its downstream genes (P < 0.05), whereas amino acid injection alleviated the starvation-induced decreases in transcript levels of these genes. These results suggested that arginine and leucine activated the TOR signaling pathway, promoted protein and lipid syntheses, and alleviated the pathway changes induced by starvation.https://doi.org/10.1038/s41598-023-50300-3 |
| spellingShingle | Xilin Dai Xuenan Li Danhui Yin Xin Chen Linwei Wang Luyao Pang Yuanshuai Fu Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid production Scientific Reports |
| title | Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid production |
| title_full | Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid production |
| title_fullStr | Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid production |
| title_full_unstemmed | Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid production |
| title_short | Identification and characterization of TOR in Macrobrachium rosenbergii and its role in muscle protein and lipid production |
| title_sort | identification and characterization of tor in macrobrachium rosenbergii and its role in muscle protein and lipid production |
| url | https://doi.org/10.1038/s41598-023-50300-3 |
| work_keys_str_mv | AT xilindai identificationandcharacterizationoftorinmacrobrachiumrosenbergiianditsroleinmuscleproteinandlipidproduction AT xuenanli identificationandcharacterizationoftorinmacrobrachiumrosenbergiianditsroleinmuscleproteinandlipidproduction AT danhuiyin identificationandcharacterizationoftorinmacrobrachiumrosenbergiianditsroleinmuscleproteinandlipidproduction AT xinchen identificationandcharacterizationoftorinmacrobrachiumrosenbergiianditsroleinmuscleproteinandlipidproduction AT linweiwang identificationandcharacterizationoftorinmacrobrachiumrosenbergiianditsroleinmuscleproteinandlipidproduction AT luyaopang identificationandcharacterizationoftorinmacrobrachiumrosenbergiianditsroleinmuscleproteinandlipidproduction AT yuanshuaifu identificationandcharacterizationoftorinmacrobrachiumrosenbergiianditsroleinmuscleproteinandlipidproduction |