Multiplexed Genome Editing via an RNA Polymerase II Promoter-Driven sgRNA Array in the Diatom Phaeodactylum tricornutum: Insights Into the Role of StLDP

Multiplexed Genome Editing via an RNA Polymerase II Promoter-Driven sgRNA Array in the Diatom Phaeodactylum tricornutum: Insights Into the Role of StLDP

CRISPR/Cas9-mediated genome editing has been demonstrated in the model diatom P. tricornutum, yet the currently available genetic tools do not combine the various advantageous features into a single, easy-to-assemble, modular construct that would allow the multiplexed targeting and creation of marke...

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Bibliographic Details
Main Authors: Yogesh Taparia, Achintya Kumar Dolui, Sammy Boussiba, Inna Khozin-Goldberg
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Plant Science
Subjects:
multiplexed genome editing
CRISPR
lipid droplet
Stramenopile-type lipid droplet protein
two-component transcriptional unit
RNA polymerase II promoter
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.784780/full

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