Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical Disinfection
Optimal disinfection protocols are fundamental to minimize bacterial resistance to the compound applied, or cross-resistance to other antimicrobials such as antibiotics. The objective is twofold: guarantee safe levels of pathogens and minimize the excess of disinfectant after a treatment. In this wo...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01259/full |
| _version_ | 1818138347719098368 |
|---|---|
| author | Míriam R. García Marta L. Cabo |
| author_facet | Míriam R. García Marta L. Cabo |
| author_sort | Míriam R. García |
| collection | DOAJ |
| description | Optimal disinfection protocols are fundamental to minimize bacterial resistance to the compound applied, or cross-resistance to other antimicrobials such as antibiotics. The objective is twofold: guarantee safe levels of pathogens and minimize the excess of disinfectant after a treatment. In this work, the disinfectant dose is optimized based on a mathematical model. The model explains and predicts the interplay between disinfectant and pathogen at different initial microbial densities (inocula) and dose concentrations. The study focuses on the disinfection of Escherichia coli with benzalkonium chloride, the most common quaternary ammonium compound. Interestingly, the specific benzalkonium chloride uptake (mean uptake per cell) decreases exponentially when the inoculum concentration increases. As a consequence, the optimal disinfectant dose increases exponentially with the initial bacterial concentration. |
| first_indexed | 2024-12-11T10:10:45Z |
| format | Article |
| id | doaj.art-134e4b7469c1454db55e34d1317ce134 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T10:10:45Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-134e4b7469c1454db55e34d1317ce1342022-12-22T01:11:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01259378390Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical DisinfectionMíriam R. García0Marta L. Cabo1Bioprocess Engineering Group, IIM-CSIC Spanish National Research Council, Vigo, SpainMicrobiology Group, IIM-CSIC Spanish National Research Council, Vigo, SpainOptimal disinfection protocols are fundamental to minimize bacterial resistance to the compound applied, or cross-resistance to other antimicrobials such as antibiotics. The objective is twofold: guarantee safe levels of pathogens and minimize the excess of disinfectant after a treatment. In this work, the disinfectant dose is optimized based on a mathematical model. The model explains and predicts the interplay between disinfectant and pathogen at different initial microbial densities (inocula) and dose concentrations. The study focuses on the disinfection of Escherichia coli with benzalkonium chloride, the most common quaternary ammonium compound. Interestingly, the specific benzalkonium chloride uptake (mean uptake per cell) decreases exponentially when the inoculum concentration increases. As a consequence, the optimal disinfectant dose increases exponentially with the initial bacterial concentration.https://www.frontiersin.org/article/10.3389/fmicb.2018.01259/fullbenzalkonium chloride (alkyldimethylbenzylammonium chloride)Escherichia colidisinfectioninactivationkinetic modelinginoculum effect |
| spellingShingle | Míriam R. García Marta L. Cabo Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical Disinfection Frontiers in Microbiology benzalkonium chloride (alkyldimethylbenzylammonium chloride) Escherichia coli disinfection inactivation kinetic modeling inoculum effect |
| title | Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical Disinfection |
| title_full | Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical Disinfection |
| title_fullStr | Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical Disinfection |
| title_full_unstemmed | Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical Disinfection |
| title_short | Optimization of E. coli Inactivation by Benzalkonium Chloride Reveals the Importance of Quantifying the Inoculum Effect on Chemical Disinfection |
| title_sort | optimization of e coli inactivation by benzalkonium chloride reveals the importance of quantifying the inoculum effect on chemical disinfection |
| topic | benzalkonium chloride (alkyldimethylbenzylammonium chloride) Escherichia coli disinfection inactivation kinetic modeling inoculum effect |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01259/full |
| work_keys_str_mv | AT miriamrgarcia optimizationofecoliinactivationbybenzalkoniumchloriderevealstheimportanceofquantifyingtheinoculumeffectonchemicaldisinfection AT martalcabo optimizationofecoliinactivationbybenzalkoniumchloriderevealstheimportanceofquantifyingtheinoculumeffectonchemicaldisinfection |