Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages
IntroductionThe excessive use of chemical fertilizer causes increasing environmental and food security crisis. Organic fertilizer improves physical and biological activities of soil. Rhizosphere microbiota, which consist of highly diverse microorganisms, play an important role in soil quality. Howev...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094034/full |
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| author | Lei Wang Lei Wang Lei Wang Handong Wang Handong Wang Handong Wang Meijin Liu Jinqing Xu Jinqing Xu Jinqing Xu Haiyan Bian Haiyan Bian Haiyan Bian Tongrui Chen Tongrui Chen Tongrui Chen Tongrui Chen En You En You En You En You Chao Deng Chao Deng Chao Deng Chao Deng Youhai Wei Tianyu Yang Yuhu Shen Yuhu Shen Yuhu Shen Yuhu Shen Yuhu Shen |
| author_facet | Lei Wang Lei Wang Lei Wang Handong Wang Handong Wang Handong Wang Meijin Liu Jinqing Xu Jinqing Xu Jinqing Xu Haiyan Bian Haiyan Bian Haiyan Bian Tongrui Chen Tongrui Chen Tongrui Chen Tongrui Chen En You En You En You En You Chao Deng Chao Deng Chao Deng Chao Deng Youhai Wei Tianyu Yang Yuhu Shen Yuhu Shen Yuhu Shen Yuhu Shen Yuhu Shen |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | IntroductionThe excessive use of chemical fertilizer causes increasing environmental and food security crisis. Organic fertilizer improves physical and biological activities of soil. Rhizosphere microbiota, which consist of highly diverse microorganisms, play an important role in soil quality. However, there is limited information about the effects of different fertilization conditions on the growth of Qingke plants and composition of the rhizosphere microbiota of the plants.MethodsIn this study, we characterized the rhizosphere microbiota of Qingke plants grown in three main Qingke-producing areas (Tibet, Qinghai, and Gansu). In each of the three areas, seven different fertilization conditions (m1–m7, m1: Unfertilized; m2: Farmer Practice; m3: 75% Farmer Practice; m4: 75% Farmer Practice +25% Organic manure; m5: 50% Farmer Practice; m6: 50% Farmer Practice +50% Organic manure; m7: 100% Organic manure) were applied. The growth and yields of the Qingke plants were also compared under the seven fertilization conditions.ResultsThere were significant differences in alpha diversity indices among the three areas. In each area, differences in fertilization conditions and differences in the growth stages of Qingke plants resulted in differences in the beta diversity of the rhizosphere microbiota. Meanwhile, in each area, fertilization conditions, soil depths, and the growth stages of Qingke plants significantly affected the relative abundance of the top 10 phyla and the top 20 bacterial genera. For most of microbial pairs established through network analysis, the significance of their correlations in each of the microbial co-occurrence networks of the three experimental sites was different. Moreover, in each of the three networks, there were significant differences in relative abundance and genera among most nodes (i.e., the genera Pseudonocardia, Skermanella, Pseudonocardia, Skermanella, Aridibacter, and Illumatobacter). The soil chemical properties (i.e., TN, TP, SOM, AN, AK, CEC, Ca, and K) were positively or negatively correlated with the relative abundance of the top 30 genera derived from the three main Qingke-producing areas (p < 0.05). Fertilization conditions markedly influenced the height of a Qingke plant, the number of spikes in a Qingke plant, the number of kernels in a spike, and the fresh weight of a Qingke plant. Considering the yield, the most effective fertilization conditions for Qingke is combining application 50% chemical fertilizer and 50% organic manure.ConclusionThe results of the present study can provide theoretical basis for practice of reducing the use of chemical fertilizer in agriculture. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-134fbe7728cc46728df8dcfa433524bf2023-08-08T13:12:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.10940341094034Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stagesLei Wang0Lei Wang1Lei Wang2Handong Wang3Handong Wang4Handong Wang5Meijin Liu6Jinqing Xu7Jinqing Xu8Jinqing Xu9Haiyan Bian10Haiyan Bian11Haiyan Bian12Tongrui Chen13Tongrui Chen14Tongrui Chen15Tongrui Chen16En You17En You18En You19En You20Chao Deng21Chao Deng22Chao Deng23Chao Deng24Youhai Wei25Tianyu Yang26Yuhu Shen27Yuhu Shen28Yuhu Shen29Yuhu Shen30Yuhu Shen31Laboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaLaboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaGannan Institute of Agricultural Sciences, Hezuo, ChinaLaboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaLaboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaLaboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaLaboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaLaboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaAcademy of Agriculture and Forestry Science, Qinghai University, Xining, ChinaCrop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, ChinaLaboratory for Research and Utilization of Qinghai Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaInnovation Academy for Seed Design, Chinese Academy of Sciences, Xining, ChinaIntroductionThe excessive use of chemical fertilizer causes increasing environmental and food security crisis. Organic fertilizer improves physical and biological activities of soil. Rhizosphere microbiota, which consist of highly diverse microorganisms, play an important role in soil quality. However, there is limited information about the effects of different fertilization conditions on the growth of Qingke plants and composition of the rhizosphere microbiota of the plants.MethodsIn this study, we characterized the rhizosphere microbiota of Qingke plants grown in three main Qingke-producing areas (Tibet, Qinghai, and Gansu). In each of the three areas, seven different fertilization conditions (m1–m7, m1: Unfertilized; m2: Farmer Practice; m3: 75% Farmer Practice; m4: 75% Farmer Practice +25% Organic manure; m5: 50% Farmer Practice; m6: 50% Farmer Practice +50% Organic manure; m7: 100% Organic manure) were applied. The growth and yields of the Qingke plants were also compared under the seven fertilization conditions.ResultsThere were significant differences in alpha diversity indices among the three areas. In each area, differences in fertilization conditions and differences in the growth stages of Qingke plants resulted in differences in the beta diversity of the rhizosphere microbiota. Meanwhile, in each area, fertilization conditions, soil depths, and the growth stages of Qingke plants significantly affected the relative abundance of the top 10 phyla and the top 20 bacterial genera. For most of microbial pairs established through network analysis, the significance of their correlations in each of the microbial co-occurrence networks of the three experimental sites was different. Moreover, in each of the three networks, there were significant differences in relative abundance and genera among most nodes (i.e., the genera Pseudonocardia, Skermanella, Pseudonocardia, Skermanella, Aridibacter, and Illumatobacter). The soil chemical properties (i.e., TN, TP, SOM, AN, AK, CEC, Ca, and K) were positively or negatively correlated with the relative abundance of the top 30 genera derived from the three main Qingke-producing areas (p < 0.05). Fertilization conditions markedly influenced the height of a Qingke plant, the number of spikes in a Qingke plant, the number of kernels in a spike, and the fresh weight of a Qingke plant. Considering the yield, the most effective fertilization conditions for Qingke is combining application 50% chemical fertilizer and 50% organic manure.ConclusionThe results of the present study can provide theoretical basis for practice of reducing the use of chemical fertilizer in agriculture.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094034/fullQingkerhizosphere microbiotafertilization conditiongeographical locationdiversity |
| spellingShingle | Lei Wang Lei Wang Lei Wang Handong Wang Handong Wang Handong Wang Meijin Liu Jinqing Xu Jinqing Xu Jinqing Xu Haiyan Bian Haiyan Bian Haiyan Bian Tongrui Chen Tongrui Chen Tongrui Chen Tongrui Chen En You En You En You En You Chao Deng Chao Deng Chao Deng Chao Deng Youhai Wei Tianyu Yang Yuhu Shen Yuhu Shen Yuhu Shen Yuhu Shen Yuhu Shen Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages Frontiers in Microbiology Qingke rhizosphere microbiota fertilization condition geographical location diversity |
| title | Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages |
| title_full | Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages |
| title_fullStr | Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages |
| title_full_unstemmed | Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages |
| title_short | Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages |
| title_sort | effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of qingke hordeum vulgare l plants in different growth stages |
| topic | Qingke rhizosphere microbiota fertilization condition geographical location diversity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094034/full |
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