Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan
Background and Aim: In Kazakhstan, the study of Echinococcus infection among farm animals is crucial to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of Echinococcus among cattle's in Kazakhstan, which makes it difficult...
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Veterinary World
2024-04-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.17/April-2024/4.pdf |
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| author | Rabiga Uakhit Sofiya Yalysheva Aida Abdybekova Ainura Smagulova Lyudmila Lider Karina Jazina Aidana Tautanova Vladimir Kiyan |
| author_facet | Rabiga Uakhit Sofiya Yalysheva Aida Abdybekova Ainura Smagulova Lyudmila Lider Karina Jazina Aidana Tautanova Vladimir Kiyan |
| author_sort | Rabiga Uakhit |
| collection | DOAJ |
| description | Background and Aim: In Kazakhstan, the study of Echinococcus infection among farm animals is crucial to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of Echinococcus among cattle's in Kazakhstan, which makes it difficult to conduct a comparative analysis of the epidemiological situation among livestock animals. The present study aimed to molecularly identify the species and haplotypes of the E. granulosus complex infecting cattle in Kazakhstan and investigate their genetic variation relative to mitochondrial (mt) targets.
Materials and Methods: Individual cyst isolates (n = 700) were collected from infected cattle lungs and livers after slaughter from the slaughterhouse. Total DNA was extracted from the germinal layers of the cyst from each isolate. This DNA sequenced partial mt genes of cytochrome c oxidase 1 (450 bp) and NADH dehydrogenase 1 (1200 bp).
Results: We determined that all the sequences were detected as E. granulosus s.s., of which 69 (94.5%) samples belonged to G1, and only 4 (5.4%) samples belonged to the G3 genotype. After bioinformatic analysis, 38 haplotypes were identified.
Conclusion: Our findings revealed that the G1 genotype of E. granulosus s.s. is the predominant cattle genotype in Kazakhstan. However, only one region showed the presence of two genotypes G1 and G3, in the sequence, which suggests that further research is needed to investigate the epidemiology of Echinococcus infection in cattle in Kazakhstan. |
| first_indexed | 2024-04-24T11:34:50Z |
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| id | doaj.art-13508a005c8e4983a3ae82ea3a4dbcf1 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-24T11:34:50Z |
| publishDate | 2024-04-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-13508a005c8e4983a3ae82ea3a4dbcf12024-04-10T06:45:18ZengVeterinary WorldVeterinary World0972-89882231-09162024-04-0117476377010.14202/vetworld.2024.763-770Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in KazakhstanRabiga Uakhit0https://orcid.org/0000-0001-7737-7162Sofiya Yalysheva1https://orcid.org/0000-0001-6632-855XAida Abdybekova2https://orcid.org/0000-0002-3307-7237Ainura Smagulova3https://orcid.org/0000-0002-3067-3666Lyudmila Lider4https://orcid.org/0000-0001-5842-0751Karina Jazina5https://orcid.org/0009-0001-8858-0753Aidana Tautanova6https://orcid.org/0000-0001-8971-1455Vladimir Kiyan7https://orcid.org/0000-0001-9787-9151Laboratory of Biodiversity and Genetic Resources, National Center for Biotechnology, Astana, Kazakhstan, Laboratory of Parasitology, Department of Veterinary Medicine, S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan.Department of Molecular Biology and Genetics, al-Farabi Kazakh National University, Almaty, Kazakhstan.Laboratory of Biodiversity and Genetic Resources, National Center for Biotechnology, Astana, Kazakhstan, Laboratory of Parasitology and Mycology, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan.Laboratory of Biodiversity and Genetic Resources, National Center for Biotechnology, Astana, Kazakhstan.Laboratory of Biodiversity and Genetic Resources, National Center for Biotechnology, Astana, Kazakhstan, Laboratory of Parasitology, Department of Veterinary Medicine, S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan.Laboratory of Parasitology, Department of Veterinary Medicine, S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan.Department of Microbiology and Virology, Astana Medical University, Astana, Kazakhstan, Department of Scientific and Analytical Work, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan.Laboratory of Biodiversity and Genetic Resources, National Center for Biotechnology, Astana, Kazakhstan.Background and Aim: In Kazakhstan, the study of Echinococcus infection among farm animals is crucial to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of Echinococcus among cattle's in Kazakhstan, which makes it difficult to conduct a comparative analysis of the epidemiological situation among livestock animals. The present study aimed to molecularly identify the species and haplotypes of the E. granulosus complex infecting cattle in Kazakhstan and investigate their genetic variation relative to mitochondrial (mt) targets. Materials and Methods: Individual cyst isolates (n = 700) were collected from infected cattle lungs and livers after slaughter from the slaughterhouse. Total DNA was extracted from the germinal layers of the cyst from each isolate. This DNA sequenced partial mt genes of cytochrome c oxidase 1 (450 bp) and NADH dehydrogenase 1 (1200 bp). Results: We determined that all the sequences were detected as E. granulosus s.s., of which 69 (94.5%) samples belonged to G1, and only 4 (5.4%) samples belonged to the G3 genotype. After bioinformatic analysis, 38 haplotypes were identified. Conclusion: Our findings revealed that the G1 genotype of E. granulosus s.s. is the predominant cattle genotype in Kazakhstan. However, only one region showed the presence of two genotypes G1 and G3, in the sequence, which suggests that further research is needed to investigate the epidemiology of Echinococcus infection in cattle in Kazakhstan.https://www.veterinaryworld.org/Vol.17/April-2024/4.pdfcattlecystic echinococcosisechinococcus granulosusgenotypeshaplotypekazakhstan |
| spellingShingle | Rabiga Uakhit Sofiya Yalysheva Aida Abdybekova Ainura Smagulova Lyudmila Lider Karina Jazina Aidana Tautanova Vladimir Kiyan Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan Veterinary World cattle cystic echinococcosis echinococcus granulosus genotypes haplotype kazakhstan |
| title | Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan |
| title_full | Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan |
| title_fullStr | Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan |
| title_full_unstemmed | Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan |
| title_short | Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan |
| title_sort | genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in kazakhstan |
| topic | cattle cystic echinococcosis echinococcus granulosus genotypes haplotype kazakhstan |
| url | https://www.veterinaryworld.org/Vol.17/April-2024/4.pdf |
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