Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatchery
Abstract A public trout hatchery in Colorado recorded repeat episodes of morbidity and mortality in early life stages of Rainbow Trout (Oncorhynchus mykiss) stocks. The water source for the affected tanks is a groundwater well with a pH of 6.3 and a ferrous iron concentration of 120 µg/L. Iron oxidi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-06-01
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| Series: | Aquaculture, Fish and Fisheries |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aff2.35 |
| _version_ | 1811333556510654464 |
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| author | Annie K. Clift Ashley M. Malmlov Colby L. Wells Pete Cadmus Paula A. Schaffer |
| author_facet | Annie K. Clift Ashley M. Malmlov Colby L. Wells Pete Cadmus Paula A. Schaffer |
| author_sort | Annie K. Clift |
| collection | DOAJ |
| description | Abstract A public trout hatchery in Colorado recorded repeat episodes of morbidity and mortality in early life stages of Rainbow Trout (Oncorhynchus mykiss) stocks. The water source for the affected tanks is a groundwater well with a pH of 6.3 and a ferrous iron concentration of 120 µg/L. Iron oxidizing bacteria (FeOB) were suspected as the underlying cause due to insoluble red particulate matter (iron oxide precipitate) in the water, and the presence of thick red slime (biofilm) that coated tanks and pipes of the facility and adhered to gills, opercula, and fins of fry. On necropsy, gill and fin clippings from moribund fish had mats of adherent red‐orange material. Histopathology revealed severe diffuse proliferative branchitis. There was abundant debris including granular to sheath‐like and stalk‐like pigmented material within the oral and branchial chambers, intimately associated with gill filaments. A Gram preparation demonstrated florid Gram‐negative bacteria within this debris. A Perls’ Prussian blue preparation revealed colocalization of abundant iron. Bacteria were identified morphologically to be of the iron oxidizing bacterial genera Gallionella and Leptothrix. This is the first known report of disease in fish associated with iron‐oxidizing bacteria. |
| first_indexed | 2024-04-13T16:54:42Z |
| format | Article |
| id | doaj.art-1350a6e263914bc0b61a03d5a154f853 |
| institution | Directory Open Access Journal |
| issn | 2693-8847 |
| language | English |
| last_indexed | 2024-04-13T16:54:42Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture, Fish and Fisheries |
| spelling | doaj.art-1350a6e263914bc0b61a03d5a154f8532022-12-22T02:38:51ZengWileyAquaculture, Fish and Fisheries2693-88472022-06-012320220710.1002/aff2.35Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatcheryAnnie K. Clift0Ashley M. Malmlov1Colby L. Wells2Pete Cadmus3Paula A. Schaffer4Department of Clinical Sciences College of Veterinary and Biomedical Sciences Colorado State University Fort Collins Colorado USAColorado Parks and Wildlife Aquatic Animal Health Laboratory Brush Colorado USAColorado Parks and Wildlife Aquatic Animal Health Laboratory Brush Colorado USAColorado Parks and Wildlife Aquatic Toxicology Laboratory Fort Collins Colorado USAVeterinary Diagnostic Laboratory Department of Microbiology Immunology and Pathology College of Veterinary and Biomedical Sciences Colorado State University Fort Collins Colorado USAAbstract A public trout hatchery in Colorado recorded repeat episodes of morbidity and mortality in early life stages of Rainbow Trout (Oncorhynchus mykiss) stocks. The water source for the affected tanks is a groundwater well with a pH of 6.3 and a ferrous iron concentration of 120 µg/L. Iron oxidizing bacteria (FeOB) were suspected as the underlying cause due to insoluble red particulate matter (iron oxide precipitate) in the water, and the presence of thick red slime (biofilm) that coated tanks and pipes of the facility and adhered to gills, opercula, and fins of fry. On necropsy, gill and fin clippings from moribund fish had mats of adherent red‐orange material. Histopathology revealed severe diffuse proliferative branchitis. There was abundant debris including granular to sheath‐like and stalk‐like pigmented material within the oral and branchial chambers, intimately associated with gill filaments. A Gram preparation demonstrated florid Gram‐negative bacteria within this debris. A Perls’ Prussian blue preparation revealed colocalization of abundant iron. Bacteria were identified morphologically to be of the iron oxidizing bacterial genera Gallionella and Leptothrix. This is the first known report of disease in fish associated with iron‐oxidizing bacteria.https://doi.org/10.1002/aff2.35biosecuritybranchitisfishGallionellahatchery managementiron‐oxidizing bacteria |
| spellingShingle | Annie K. Clift Ashley M. Malmlov Colby L. Wells Pete Cadmus Paula A. Schaffer Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatchery Aquaculture, Fish and Fisheries biosecurity branchitis fish Gallionella hatchery management iron‐oxidizing bacteria |
| title | Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatchery |
| title_full | Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatchery |
| title_fullStr | Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatchery |
| title_full_unstemmed | Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatchery |
| title_short | Branchitis and mortality in rainbow trout Oncorhynchus mykiss exposed to iron oxidizing bacteria: Diagnostics and management in a Colorado hatchery |
| title_sort | branchitis and mortality in rainbow trout oncorhynchus mykiss exposed to iron oxidizing bacteria diagnostics and management in a colorado hatchery |
| topic | biosecurity branchitis fish Gallionella hatchery management iron‐oxidizing bacteria |
| url | https://doi.org/10.1002/aff2.35 |
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