Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of Serbia
The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven <i>Salmonella</i> spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to col...
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MDPI AG
2020-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/12/886 |
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| author | Branko Jovčić Katarina Novović Brankica Filipić Maja Velhner Dalibor Todorović Kazimir Matović Zoran Rašić Sonja Nikolić Ferenc Kiškarolj Milan Kojić |
| author_facet | Branko Jovčić Katarina Novović Brankica Filipić Maja Velhner Dalibor Todorović Kazimir Matović Zoran Rašić Sonja Nikolić Ferenc Kiškarolj Milan Kojić |
| author_sort | Branko Jovčić |
| collection | DOAJ |
| description | The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven <i>Salmonella</i> spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant <i>Salmonella</i> Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common <i>S.</i> Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene <i>fosA7</i> in isolate 3842 and the <i>vgaA</i> gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the <i>phoP</i>/<i>Q</i>, <i>mgrB</i> and <i>pmrA/B</i> genes were detected. Single nucleotide polymorphisms of the <i>pmrB</i> gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed <i>Salmonella</i> Infantis isolates from central Serbia. |
| first_indexed | 2024-03-10T14:11:22Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T14:11:22Z |
| publishDate | 2020-12-01 |
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| series | Antibiotics |
| spelling | doaj.art-13562803340a4a01b6b06ff66d0b3f952023-11-21T00:06:35ZengMDPI AGAntibiotics2079-63822020-12-0191288610.3390/antibiotics9120886Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of SerbiaBranko Jovčić0Katarina Novović1Brankica Filipić2Maja Velhner3Dalibor Todorović4Kazimir Matović5Zoran Rašić6Sonja Nikolić7Ferenc Kiškarolj8Milan Kojić9Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11010 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11010 Belgrade, SerbiaFaculty of Pharmacy, University of Belgrade, 11000 Belgrade, SerbiaScientific Veterinary Institute “Novi Sad”, 21000 Novi Sad, SerbiaScientific Veterinary Institute “Novi Sad”, 21000 Novi Sad, SerbiaVeterinary Specialized Institute “Kraljevo”, 36000 Kraljevo, SerbiaVeterinary Specialized Institute “Jagodina”, 35000 Jagodina, SerbiaVeterinary Specialized Institute “Niš”, 18000 Niš, SerbiaVeterinary Specialized Institute “Subotica”, 24000 Subotica, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11010 Belgrade, SerbiaThe antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven <i>Salmonella</i> spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant <i>Salmonella</i> Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common <i>S.</i> Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene <i>fosA7</i> in isolate 3842 and the <i>vgaA</i> gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the <i>phoP</i>/<i>Q</i>, <i>mgrB</i> and <i>pmrA/B</i> genes were detected. Single nucleotide polymorphisms of the <i>pmrB</i> gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed <i>Salmonella</i> Infantis isolates from central Serbia.https://www.mdpi.com/2079-6382/9/12/886<i>Salmonella</i>colistinquinolonesresistanceWGS<i>pmrB</i> |
| spellingShingle | Branko Jovčić Katarina Novović Brankica Filipić Maja Velhner Dalibor Todorović Kazimir Matović Zoran Rašić Sonja Nikolić Ferenc Kiškarolj Milan Kojić Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of Serbia Antibiotics <i>Salmonella</i> colistin quinolones resistance WGS <i>pmrB</i> |
| title | Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of Serbia |
| title_full | Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of Serbia |
| title_fullStr | Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of Serbia |
| title_full_unstemmed | Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of Serbia |
| title_short | Genomic Characteristics of Colistin-Resistant <i>Salmonella</i> <i>enterica</i> subsp. <i>enterica</i> Serovar Infantis from Poultry Farms in the Republic of Serbia |
| title_sort | genomic characteristics of colistin resistant i salmonella i i enterica i subsp i enterica i serovar infantis from poultry farms in the republic of serbia |
| topic | <i>Salmonella</i> colistin quinolones resistance WGS <i>pmrB</i> |
| url | https://www.mdpi.com/2079-6382/9/12/886 |
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