Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city
The aim of this study was to identify and diagnose of<em> M. haemolytica</em> strains as one of the most important causes of ovine clinical mastitis in Mosul city. One hundred and thirty-three milk samples were directly obtained from the udders of ewes infected by clinical mastitis from...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2022-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://vetmedmosul.com/article_172297_f8707ce62086d9df9873723b9647145b.pdf |
| _version_ | 1818754440468889600 |
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| author | Tariq J Rasheed Dhyaa M. Jwher |
| author_facet | Tariq J Rasheed Dhyaa M. Jwher |
| author_sort | Tariq J Rasheed |
| collection | DOAJ |
| description | The aim of this study was to identify and diagnose of<em> M. haemolytica</em> strains as one of the most important causes of ovine clinical mastitis in Mosul city. One hundred and thirty-three milk samples were directly obtained from the udders of ewes infected by clinical mastitis from November 2020 to January 2021.Standard and conventional methods were followed for isolation and identification of <em>M. haemolytica</em>. Milk samples were cultured on blood agar 7% and MacConkey agar, then it was purified and was stained by Methylene blue. Later, different biochemical tests were Conducted. Molecular identification of <em>M. haemolytica</em> depending on 16srRNA gene, followed by sequencing, similarity and phylogenetic tree was generated. The results showed that 62(46.61%) of samples were positive for bacterial isolation, biochemical tests and conventional PCR technique. Sequencing results showed that the positive samples were belonged to <em>M. haemolytica</em> strains. The similarity within strain Ib001 and within strain 39433 were 100%, and 99.47% respectively. Poor management was associated with the high level of mastitis caused by <em>M. haemolytica</em>, so the application of prophylactic programs should be followed to limit the spread of the disease. |
| first_indexed | 2024-12-18T05:23:17Z |
| format | Article |
| id | doaj.art-1358cc1d61f74865ae6215d6546c8b6d |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-18T05:23:17Z |
| publishDate | 2022-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-1358cc1d61f74865ae6215d6546c8b6d2022-12-21T21:19:35ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552022-04-0136238138610.33899/ijvs.2021.130347.1805172297Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul cityTariq J Rasheed0Dhyaa M. Jwher1Research directorate, Nineveh Agriculture directorate, Ministry of Agriculture, Mosul, IraqDepartment of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, IraqThe aim of this study was to identify and diagnose of<em> M. haemolytica</em> strains as one of the most important causes of ovine clinical mastitis in Mosul city. One hundred and thirty-three milk samples were directly obtained from the udders of ewes infected by clinical mastitis from November 2020 to January 2021.Standard and conventional methods were followed for isolation and identification of <em>M. haemolytica</em>. Milk samples were cultured on blood agar 7% and MacConkey agar, then it was purified and was stained by Methylene blue. Later, different biochemical tests were Conducted. Molecular identification of <em>M. haemolytica</em> depending on 16srRNA gene, followed by sequencing, similarity and phylogenetic tree was generated. The results showed that 62(46.61%) of samples were positive for bacterial isolation, biochemical tests and conventional PCR technique. Sequencing results showed that the positive samples were belonged to <em>M. haemolytica</em> strains. The similarity within strain Ib001 and within strain 39433 were 100%, and 99.47% respectively. Poor management was associated with the high level of mastitis caused by <em>M. haemolytica</em>, so the application of prophylactic programs should be followed to limit the spread of the disease.https://vetmedmosul.com/article_172297_f8707ce62086d9df9873723b9647145b.pdfovine mastitismannheimia haemolyticamosul city |
| spellingShingle | Tariq J Rasheed Dhyaa M. Jwher Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city Iraqi Journal of Veterinary Sciences ovine mastitis mannheimia haemolytica mosul city |
| title | Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city |
| title_full | Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city |
| title_fullStr | Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city |
| title_full_unstemmed | Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city |
| title_short | Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city |
| title_sort | isolation identification and genetic analysis of mannheimia haemolytica in ovine clinical mastitis in mosul city |
| topic | ovine mastitis mannheimia haemolytica mosul city |
| url | https://vetmedmosul.com/article_172297_f8707ce62086d9df9873723b9647145b.pdf |
| work_keys_str_mv | AT tariqjrasheed isolationidentificationandgeneticanalysisofmannheimiahaemolyticainovineclinicalmastitisinmosulcity AT dhyaamjwher isolationidentificationandgeneticanalysisofmannheimiahaemolyticainovineclinicalmastitisinmosulcity |