Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166
MicroRNAs (miRNAs) play essential functions in pathogenesis of Ewing sarcoma (ES). However, the molecular mechanisms responsible for ES occurrence and development through the regulation of miRNAs remain largely unknown. This study is aimed to explore the differential expressed miRNAs and mRNAs that...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1620760 |
| _version_ | 1818140181080834048 |
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| author | Yuzhe Liu Gaoyang Chen He Liu Zhaoyan Li Qiwei Yang Xinming Gu Zhenwu Du Guizhen Zhang Jincheng Wang |
| author_facet | Yuzhe Liu Gaoyang Chen He Liu Zhaoyan Li Qiwei Yang Xinming Gu Zhenwu Du Guizhen Zhang Jincheng Wang |
| author_sort | Yuzhe Liu |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) play essential functions in pathogenesis of Ewing sarcoma (ES). However, the molecular mechanisms responsible for ES occurrence and development through the regulation of miRNAs remain largely unknown. This study is aimed to explore the differential expressed miRNAs and mRNAs that play vital roles in ES. GSE80201 miRNA and GSE68776 mRNA microarray dataset were selected to carry out a series of bioinformatics analysis such as GEO 2R, gene ontology, pathway enrichment analysis, Venn analysis and PPI network construction to predict hub genes. Furthermore, using quantitative real-time PCR, RNA interference and luciferase reporter assay we demonstrated that activated leukocyte cell adhesion molecule (ALCAM/CD166) is a direct target of miR-21-3p in human ES cell lines. Our results suggest that the miR-21/CD166 axis has the potential to serve as both diagnostic markers and therapeutic targets for ES. |
| first_indexed | 2024-12-11T10:39:54Z |
| format | Article |
| id | doaj.art-13595152309c416598f5fabb5fa00d60 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-12-11T10:39:54Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-13595152309c416598f5fabb5fa00d602022-12-22T01:10:37ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014712114212210.1080/21691401.2019.1620760Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166Yuzhe Liu0Gaoyang Chen1He Liu2Zhaoyan Li3Qiwei Yang4Xinming Gu5Zhenwu Du6Guizhen Zhang7Jincheng Wang8Department of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaDepartment of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaDepartment of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaDepartment of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaDepartment of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaDepartment of Oral Implantology of School and Hospital of Stomatology, Jilin University, Changchun, ChinaDepartment of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaDepartment of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaDepartment of Orthopaedics of the Second Hospital, Jilin University, Changchun, ChinaMicroRNAs (miRNAs) play essential functions in pathogenesis of Ewing sarcoma (ES). However, the molecular mechanisms responsible for ES occurrence and development through the regulation of miRNAs remain largely unknown. This study is aimed to explore the differential expressed miRNAs and mRNAs that play vital roles in ES. GSE80201 miRNA and GSE68776 mRNA microarray dataset were selected to carry out a series of bioinformatics analysis such as GEO 2R, gene ontology, pathway enrichment analysis, Venn analysis and PPI network construction to predict hub genes. Furthermore, using quantitative real-time PCR, RNA interference and luciferase reporter assay we demonstrated that activated leukocyte cell adhesion molecule (ALCAM/CD166) is a direct target of miR-21-3p in human ES cell lines. Our results suggest that the miR-21/CD166 axis has the potential to serve as both diagnostic markers and therapeutic targets for ES.https://www.tandfonline.com/doi/10.1080/21691401.2019.1620760Ewing sarcomamiRNA expressionmiR-21-3pCD166target genes |
| spellingShingle | Yuzhe Liu Gaoyang Chen He Liu Zhaoyan Li Qiwei Yang Xinming Gu Zhenwu Du Guizhen Zhang Jincheng Wang Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166 Artificial Cells, Nanomedicine, and Biotechnology Ewing sarcoma miRNA expression miR-21-3p CD166 target genes |
| title | Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166 |
| title_full | Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166 |
| title_fullStr | Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166 |
| title_full_unstemmed | Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166 |
| title_short | Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166 |
| title_sort | integrated bioinformatics analysis of mirna expression in ewing sarcoma and potential regulatory effects of mir 21 via targeting alcam cd166 |
| topic | Ewing sarcoma miRNA expression miR-21-3p CD166 target genes |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1620760 |
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