A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months
An effective and rapid diagnosis has great importance in tackling the ongoing COVID-19 pandemic through isolation of the infected individuals to curb the transmission and initiation of specialized treatment for the disease. It has been proven that enhanced testing capacities contribute to efficientl...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.858555/full |
| _version_ | 1817968678719717376 |
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| author | Prerna Mandhan Mansi Sharma Sushmita Pandey Neha Chandel Nidhi Chourasia Amit Moun Divyani Sharma Rubee Sukar Niyati Singh Shubhangi Mathur Aarti Kotnala Neetu Negi Ashish Gupta Anuj Kumar R. Suresh Kumar Pramod Kumar Shalini Singh |
| author_facet | Prerna Mandhan Mansi Sharma Sushmita Pandey Neha Chandel Nidhi Chourasia Amit Moun Divyani Sharma Rubee Sukar Niyati Singh Shubhangi Mathur Aarti Kotnala Neetu Negi Ashish Gupta Anuj Kumar R. Suresh Kumar Pramod Kumar Shalini Singh |
| author_sort | Prerna Mandhan |
| collection | DOAJ |
| description | An effective and rapid diagnosis has great importance in tackling the ongoing COVID-19 pandemic through isolation of the infected individuals to curb the transmission and initiation of specialized treatment for the disease. It has been proven that enhanced testing capacities contribute to efficiently curbing SARS-CoV-2 transmission during the initial phases of the outbreaks. RT-qPCR is considered a gold standard for the diagnosis of COVID-19. However, in resource-limited countries expenses for molecular diagnosis limits the diagnostic capacities. Here, we present interventions of two pooling strategies as 5 sample pooling (P-5) and 10 sample pooling (P-10) in a high-throughput COVID-19 diagnostic laboratory to enhance throughput and save resources and time over a period of 6 months. The diagnostic capacity was scaled-up 2.15-folds in P-5 and 1.8-fold in P-10, reagents (toward RNA extraction and RT-qPCR) were preserved at 75.24% in P-5 and 86.21% in P-10, and time saved was 6,290.93 h in P-5 and 3147.3 h in P-10. |
| first_indexed | 2024-04-13T20:11:30Z |
| format | Article |
| id | doaj.art-135b23bcd38740f9bcc2ae1d7e926aa3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T20:11:30Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-135b23bcd38740f9bcc2ae1d7e926aa32022-12-22T02:31:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.858555858555A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 MonthsPrerna MandhanMansi SharmaSushmita PandeyNeha ChandelNidhi ChourasiaAmit MounDivyani SharmaRubee SukarNiyati SinghShubhangi MathurAarti KotnalaNeetu NegiAshish GuptaAnuj KumarR. Suresh KumarPramod KumarShalini SinghAn effective and rapid diagnosis has great importance in tackling the ongoing COVID-19 pandemic through isolation of the infected individuals to curb the transmission and initiation of specialized treatment for the disease. It has been proven that enhanced testing capacities contribute to efficiently curbing SARS-CoV-2 transmission during the initial phases of the outbreaks. RT-qPCR is considered a gold standard for the diagnosis of COVID-19. However, in resource-limited countries expenses for molecular diagnosis limits the diagnostic capacities. Here, we present interventions of two pooling strategies as 5 sample pooling (P-5) and 10 sample pooling (P-10) in a high-throughput COVID-19 diagnostic laboratory to enhance throughput and save resources and time over a period of 6 months. The diagnostic capacity was scaled-up 2.15-folds in P-5 and 1.8-fold in P-10, reagents (toward RNA extraction and RT-qPCR) were preserved at 75.24% in P-5 and 86.21% in P-10, and time saved was 6,290.93 h in P-5 and 3147.3 h in P-10.https://www.frontiersin.org/articles/10.3389/fmicb.2022.858555/fullCOVID-19SARS-CoV-2pooling interventionshigh-throughput diagnosisRT-qPCR–real-time quantitative polymerase chain reaction |
| spellingShingle | Prerna Mandhan Mansi Sharma Sushmita Pandey Neha Chandel Nidhi Chourasia Amit Moun Divyani Sharma Rubee Sukar Niyati Singh Shubhangi Mathur Aarti Kotnala Neetu Negi Ashish Gupta Anuj Kumar R. Suresh Kumar Pramod Kumar Shalini Singh A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months Frontiers in Microbiology COVID-19 SARS-CoV-2 pooling interventions high-throughput diagnosis RT-qPCR–real-time quantitative polymerase chain reaction |
| title | A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months |
| title_full | A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months |
| title_fullStr | A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months |
| title_full_unstemmed | A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months |
| title_short | A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months |
| title_sort | regional pooling intervention in a high throughput covid 19 diagnostic laboratory to enhance throughput save resources and time over a period of 6 months |
| topic | COVID-19 SARS-CoV-2 pooling interventions high-throughput diagnosis RT-qPCR–real-time quantitative polymerase chain reaction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.858555/full |
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