Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin
The stingless bee <i>Partamona helleri</i> plays a role in pollinating both native and cultivated plants in the Neotropics. However, its populations can be reduced by the pyrethroid insecticide lambda-cyhalothrin. This compound may cross the intestinal barrier and circulate through the h...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/12/8/1510 |
| _version_ | 1797585657287147520 |
|---|---|
| author | João Victor de Oliveira Motta Lenise Silva Carneiro Luís Carlos Martínez Daniel Silva Sena Bastos Matheus Tudor Candido Santos Resende Bárbara Monteiro Castro Castro Mariana Machado Neves José Cola Zanuncio José Eduardo Serrão |
| author_facet | João Victor de Oliveira Motta Lenise Silva Carneiro Luís Carlos Martínez Daniel Silva Sena Bastos Matheus Tudor Candido Santos Resende Bárbara Monteiro Castro Castro Mariana Machado Neves José Cola Zanuncio José Eduardo Serrão |
| author_sort | João Victor de Oliveira Motta |
| collection | DOAJ |
| description | The stingless bee <i>Partamona helleri</i> plays a role in pollinating both native and cultivated plants in the Neotropics. However, its populations can be reduced by the pyrethroid insecticide lambda-cyhalothrin. This compound may cross the intestinal barrier and circulate through the hemolymph, affecting various non-target bee organs. The aim of the present study was to assess the extent of cellular damage in the midgut and the resulting oxidative stress caused by lambda-cyhalothrin in <i>P. helleri</i> workers. Bees were orally exposed to lambda-cyhalothrin. The lethal concentration at which 50% of the bees died (LC<sub>50</sub>) was 0.043 mg a.i. L<sup>−1</sup>. The <i>P. helleri</i> workers were fed this concentration of lambda-cyhalothrin and their midguts were evaluated. The results revealed signs of damage in the midgut epithelium, including pyknotic nuclei, cytoplasm vacuolization, changes in the striated border, and the release of cell fragments, indicating that the midgut was compromised. Furthermore, the ingestion of lambda-cyhalothrin led to an increase in the activity of the detoxification enzyme superoxide dismutase and the levels of the NO<sub>2</sub>/NO<sub>3</sub> markers, indicating oxidative stress. Conversely, the activities of the catalase and glutathione S-transferase enzymes decreased, supporting the occurrence of oxidative stress. In conclusion, the ingestion of lambda-cyhalothrin by <i>P. helleri</i> workers resulted in damage to their midguts and induced oxidative stress. |
| first_indexed | 2024-03-11T00:10:12Z |
| format | Article |
| id | doaj.art-135d9195c26f4d82b79e4ec7c89538d2 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T00:10:12Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-135d9195c26f4d82b79e4ec7c89538d22023-11-19T00:00:55ZengMDPI AGAntioxidants2076-39212023-07-01128151010.3390/antiox12081510Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-CyhalothrinJoão Victor de Oliveira Motta0Lenise Silva Carneiro1Luís Carlos Martínez2Daniel Silva Sena Bastos3Matheus Tudor Candido Santos Resende4Bárbara Monteiro Castro Castro5Mariana Machado Neves6José Cola Zanuncio7José Eduardo Serrão8Department of General Biology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilDepartment of General Biology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilFaculdad de Ciencias Agricolas, Universidad de Narino, Pasto 522020, ColombiaDepartment of General Biology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilDepartment of General Biology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilDepartment of Entomology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilDepartment of General Biology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilDepartment of Entomology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilDepartment of General Biology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, BrazilThe stingless bee <i>Partamona helleri</i> plays a role in pollinating both native and cultivated plants in the Neotropics. However, its populations can be reduced by the pyrethroid insecticide lambda-cyhalothrin. This compound may cross the intestinal barrier and circulate through the hemolymph, affecting various non-target bee organs. The aim of the present study was to assess the extent of cellular damage in the midgut and the resulting oxidative stress caused by lambda-cyhalothrin in <i>P. helleri</i> workers. Bees were orally exposed to lambda-cyhalothrin. The lethal concentration at which 50% of the bees died (LC<sub>50</sub>) was 0.043 mg a.i. L<sup>−1</sup>. The <i>P. helleri</i> workers were fed this concentration of lambda-cyhalothrin and their midguts were evaluated. The results revealed signs of damage in the midgut epithelium, including pyknotic nuclei, cytoplasm vacuolization, changes in the striated border, and the release of cell fragments, indicating that the midgut was compromised. Furthermore, the ingestion of lambda-cyhalothrin led to an increase in the activity of the detoxification enzyme superoxide dismutase and the levels of the NO<sub>2</sub>/NO<sub>3</sub> markers, indicating oxidative stress. Conversely, the activities of the catalase and glutathione S-transferase enzymes decreased, supporting the occurrence of oxidative stress. In conclusion, the ingestion of lambda-cyhalothrin by <i>P. helleri</i> workers resulted in damage to their midguts and induced oxidative stress.https://www.mdpi.com/2076-3921/12/8/1510cytotoxicityhistopathologypollinators |
| spellingShingle | João Victor de Oliveira Motta Lenise Silva Carneiro Luís Carlos Martínez Daniel Silva Sena Bastos Matheus Tudor Candido Santos Resende Bárbara Monteiro Castro Castro Mariana Machado Neves José Cola Zanuncio José Eduardo Serrão Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin Antioxidants cytotoxicity histopathology pollinators |
| title | Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin |
| title_full | Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin |
| title_fullStr | Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin |
| title_full_unstemmed | Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin |
| title_short | Midgut Cell Damage and Oxidative Stress in <i>Partamona helleri</i> (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin |
| title_sort | midgut cell damage and oxidative stress in i partamona helleri i hymenoptera apidae workers caused by the insecticide lambda cyhalothrin |
| topic | cytotoxicity histopathology pollinators |
| url | https://www.mdpi.com/2076-3921/12/8/1510 |
| work_keys_str_mv | AT joaovictordeoliveiramotta midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT lenisesilvacarneiro midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT luiscarlosmartinez midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT danielsilvasenabastos midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT matheustudorcandidosantosresende midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT barbaramonteirocastrocastro midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT marianamachadoneves midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT josecolazanuncio midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin AT joseeduardoserrao midgutcelldamageandoxidativestressinipartamonahelleriihymenopteraapidaeworkerscausedbytheinsecticidelambdacyhalothrin |