Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitro
Aristolochic acids (AAs) are the active components of herbal drugs derived from Aristolochia species that have been used for medicinal purposes since antiquity. However, AAs have recently been discovered to be highly nephrotoxic and induced urothelial cancer in humans and malignant tumors in the kid...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | Genomics Data |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213596015000914 |
| _version_ | 1818520055403511808 |
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| author | Katarzyna M. Bloch Andrew Evans Edward A. Lock |
| author_facet | Katarzyna M. Bloch Andrew Evans Edward A. Lock |
| author_sort | Katarzyna M. Bloch |
| collection | DOAJ |
| description | Aristolochic acids (AAs) are the active components of herbal drugs derived from Aristolochia species that have been used for medicinal purposes since antiquity. However, AAs have recently been discovered to be highly nephrotoxic and induced urothelial cancer in humans and malignant tumors in the kidney and urinary tract of rodents. In this study, we exposed rat renal proximal tubule cells in vitro to a sub-cytotoxic level of AAs at three different time points (6 h, 24 h and 72 h). We then analyzed the gene expression profile after the compound exposure. Functional analysis with Ingenuity Pathways Analysis and DAVID tools revealed that at the late time point (72 h) there are many significantly altered genes involved in cancer-related pathways such as p53 signaling.
MIAMI-compliant microarray data are deposited in the NCBI GEO database under accession number GSE68687 and can be found at: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68687. |
| first_indexed | 2024-12-11T01:32:17Z |
| format | Article |
| id | doaj.art-135f56b5676c4a53afac5753dad6b015 |
| institution | Directory Open Access Journal |
| issn | 2213-5960 |
| language | English |
| last_indexed | 2024-12-11T01:32:17Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics Data |
| spelling | doaj.art-135f56b5676c4a53afac5753dad6b0152022-12-22T01:25:19ZengElsevierGenomics Data2213-59602015-09-015C25425610.1016/j.gdata.2015.05.028Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitroKatarzyna M. BlochAndrew EvansEdward A. LockAristolochic acids (AAs) are the active components of herbal drugs derived from Aristolochia species that have been used for medicinal purposes since antiquity. However, AAs have recently been discovered to be highly nephrotoxic and induced urothelial cancer in humans and malignant tumors in the kidney and urinary tract of rodents. In this study, we exposed rat renal proximal tubule cells in vitro to a sub-cytotoxic level of AAs at three different time points (6 h, 24 h and 72 h). We then analyzed the gene expression profile after the compound exposure. Functional analysis with Ingenuity Pathways Analysis and DAVID tools revealed that at the late time point (72 h) there are many significantly altered genes involved in cancer-related pathways such as p53 signaling. MIAMI-compliant microarray data are deposited in the NCBI GEO database under accession number GSE68687 and can be found at: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68687.http://www.sciencedirect.com/science/article/pii/S2213596015000914 |
| spellingShingle | Katarzyna M. Bloch Andrew Evans Edward A. Lock Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitro Genomics Data |
| title | Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitro |
| title_full | Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitro |
| title_fullStr | Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitro |
| title_full_unstemmed | Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitro |
| title_short | Aristolochic acids — Induced transcriptomic responses in rat renal proximal tubule cells in vitro |
| title_sort | aristolochic acids induced transcriptomic responses in rat renal proximal tubule cells in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S2213596015000914 |
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