Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp.
Assam tea plants (<i>Camellia sinensis</i> var. <i>assamica</i>) or Miang are found in plantations and forests of Northern Thailand. Leaf fermentation has been performed for centuries, but little information is available about their associated microbial community. One hundred...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1585 |
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| author | Patthanasak Rungsirivanich Witsanu Supandee Wirapong Futui Vipanee Chumsai-Na-Ayudhya Chaowarin Yodsombat Narumol Thongwai |
| author_facet | Patthanasak Rungsirivanich Witsanu Supandee Wirapong Futui Vipanee Chumsai-Na-Ayudhya Chaowarin Yodsombat Narumol Thongwai |
| author_sort | Patthanasak Rungsirivanich |
| collection | DOAJ |
| description | Assam tea plants (<i>Camellia sinensis</i> var. <i>assamica</i>) or Miang are found in plantations and forests of Northern Thailand. Leaf fermentation has been performed for centuries, but little information is available about their associated microbial community. One hundred and fifty-seven bacterial isolates were isolated from 62 Assam tea leaf samples collected from 6 provinces of Northern Thailand and classified within the phyla of Firmicutes, Actinobacteria, and Proteobacteria. Phayao and Phrae provinces exhibited the highest and the lowest bacterial diversities, respectively. The bacterial community structural pattern demonstrated significant differences between the west and the east sides. Since some <i>Bacillus</i> spp. have been reported to be involved in fermented Miang, <i>Bacillus</i> spp. isolated in this study were chosen for further elucidation. <i>Bacillus siamensis</i> ML122-2 exhibited a growth inhibitory effect against <i>Staphylococcus aureus</i> ATCC 25923 and MRSA DMST 20625, and the highest survival ability in simulated gastric and intestinal fluids (32.3 and 99.7%, respectively), autoaggregation (93.2%), cell surface hydrophobicity (50.0%), and bacterial adherence with Vero cells (75.8% of the control <i>Lactiplantibacillus</i><i>plantarum</i> FM03-1). This <i>B. siamensis</i> ML122-2 is a promising probiotic to be used in the food industry and seems to have potential antibacterial properties relevant for the treatment of antibiotic-resistant infections. |
| first_indexed | 2024-03-10T15:37:05Z |
| format | Article |
| id | doaj.art-135f70646bcc469a897b2b6fbb99230c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:37:05Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-135f70646bcc469a897b2b6fbb99230c2023-11-20T17:07:38ZengMDPI AGMicroorganisms2076-26072020-10-01810158510.3390/microorganisms8101585Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp.Patthanasak Rungsirivanich0Witsanu Supandee1Wirapong Futui2Vipanee Chumsai-Na-Ayudhya3Chaowarin Yodsombat4Narumol Thongwai5Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDarunsikkhalai School, King Mongkut’s University of Technology Thonburi, Bangkok 10150, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandProduction and Water Quality Control Division, Provincial Waterworks Authority Region 2, Saraburi 18000, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandAssam tea plants (<i>Camellia sinensis</i> var. <i>assamica</i>) or Miang are found in plantations and forests of Northern Thailand. Leaf fermentation has been performed for centuries, but little information is available about their associated microbial community. One hundred and fifty-seven bacterial isolates were isolated from 62 Assam tea leaf samples collected from 6 provinces of Northern Thailand and classified within the phyla of Firmicutes, Actinobacteria, and Proteobacteria. Phayao and Phrae provinces exhibited the highest and the lowest bacterial diversities, respectively. The bacterial community structural pattern demonstrated significant differences between the west and the east sides. Since some <i>Bacillus</i> spp. have been reported to be involved in fermented Miang, <i>Bacillus</i> spp. isolated in this study were chosen for further elucidation. <i>Bacillus siamensis</i> ML122-2 exhibited a growth inhibitory effect against <i>Staphylococcus aureus</i> ATCC 25923 and MRSA DMST 20625, and the highest survival ability in simulated gastric and intestinal fluids (32.3 and 99.7%, respectively), autoaggregation (93.2%), cell surface hydrophobicity (50.0%), and bacterial adherence with Vero cells (75.8% of the control <i>Lactiplantibacillus</i><i>plantarum</i> FM03-1). This <i>B. siamensis</i> ML122-2 is a promising probiotic to be used in the food industry and seems to have potential antibacterial properties relevant for the treatment of antibiotic-resistant infections.https://www.mdpi.com/2076-2607/8/10/1585antibiotic resistancebacterial adhesiondiversityMiangmicroecosystemprobiotics |
| spellingShingle | Patthanasak Rungsirivanich Witsanu Supandee Wirapong Futui Vipanee Chumsai-Na-Ayudhya Chaowarin Yodsombat Narumol Thongwai Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp. Microorganisms antibiotic resistance bacterial adhesion diversity Miang microecosystem probiotics |
| title | Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp. |
| title_full | Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp. |
| title_fullStr | Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp. |
| title_full_unstemmed | Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp. |
| title_short | Culturable Bacterial Community on Leaves of Assam Tea (<i>Camellia sinensis</i> var. <i>assamica</i>) in Thailand and Human Probiotic Potential of Isolated <i>Bacillus</i> spp. |
| title_sort | culturable bacterial community on leaves of assam tea i camellia sinensis i var i assamica i in thailand and human probiotic potential of isolated i bacillus i spp |
| topic | antibiotic resistance bacterial adhesion diversity Miang microecosystem probiotics |
| url | https://www.mdpi.com/2076-2607/8/10/1585 |
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