Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in Mice
Vagus nerve stimulation (VNS) is considered a potential method for anti-inflammation due to the involvement of the VN in the cholinergic anti-inflammatory pathway (CAP) formation of a connection between the central nervous system and peripheral immune cells that help relieve inflammation. However, w...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/2/247 |
| _version_ | 1797482450550521856 |
|---|---|
| author | Yoon-Young Go Won-Min Ju Chan-Mi Lee Sung-Won Chae Jae-Jun Song |
| author_facet | Yoon-Young Go Won-Min Ju Chan-Mi Lee Sung-Won Chae Jae-Jun Song |
| author_sort | Yoon-Young Go |
| collection | DOAJ |
| description | Vagus nerve stimulation (VNS) is considered a potential method for anti-inflammation due to the involvement of the VN in the cholinergic anti-inflammatory pathway (CAP) formation of a connection between the central nervous system and peripheral immune cells that help relieve inflammation. However, whether a non-invasive transcutaneous auricular VNS (taVNS) modulates the inflammation levels via altering the parameter of taVNS is poorly understood. This study aimed to determine the differential inhibitory effects of taVNS on lipopolysaccharide (LPS)-induced systemic inflammation using electrical stimulation parameters such as pulse frequency and time. The taVNS-promoted CAP activity significantly recovered LPS-induced tissue injuries (lung, spleen, and intestine) and decreased inflammatory cytokine levels and tissue-infiltrated immune cells. Interestingly, the anti-inflammatory capacity of taVNS with 15 Hz was much higher than that of taVNS with 25 Hz. When a cytokine array was used to investigate the changes of inflammation and immune response-related cytokines/chemokines expression in taVNS with 15 Hz or 25 Hz treatment in LPS-induced endotoxemia in mice, most of the expression of cytokines/chemokines associated with pro-inflammation was severely decreased in taVNS with 15 Hz compared to 25 Hz. This study demonstrated that the taVNS parameter could differentially modulate the inflammation levels of animals, suggesting the importance of taVNS parameter selection for use in feasible interventions for acute inflammation treatment. |
| first_indexed | 2024-03-09T22:33:30Z |
| format | Article |
| id | doaj.art-135fcc129c02461e9801332195701ea9 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T22:33:30Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-135fcc129c02461e9801332195701ea92023-11-23T18:52:38ZengMDPI AGBiomedicines2227-90592022-01-0110224710.3390/biomedicines10020247Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in MiceYoon-Young Go0Won-Min Ju1Chan-Mi Lee2Sung-Won Chae3Jae-Jun Song4Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Guro Hospital, 80 Guro-dong, Guro-gu, Seoul 08308, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Korea University Guro Hospital, 80 Guro-dong, Guro-gu, Seoul 08308, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Korea University Guro Hospital, 80 Guro-dong, Guro-gu, Seoul 08308, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Korea University Guro Hospital, 80 Guro-dong, Guro-gu, Seoul 08308, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Korea University Guro Hospital, 80 Guro-dong, Guro-gu, Seoul 08308, KoreaVagus nerve stimulation (VNS) is considered a potential method for anti-inflammation due to the involvement of the VN in the cholinergic anti-inflammatory pathway (CAP) formation of a connection between the central nervous system and peripheral immune cells that help relieve inflammation. However, whether a non-invasive transcutaneous auricular VNS (taVNS) modulates the inflammation levels via altering the parameter of taVNS is poorly understood. This study aimed to determine the differential inhibitory effects of taVNS on lipopolysaccharide (LPS)-induced systemic inflammation using electrical stimulation parameters such as pulse frequency and time. The taVNS-promoted CAP activity significantly recovered LPS-induced tissue injuries (lung, spleen, and intestine) and decreased inflammatory cytokine levels and tissue-infiltrated immune cells. Interestingly, the anti-inflammatory capacity of taVNS with 15 Hz was much higher than that of taVNS with 25 Hz. When a cytokine array was used to investigate the changes of inflammation and immune response-related cytokines/chemokines expression in taVNS with 15 Hz or 25 Hz treatment in LPS-induced endotoxemia in mice, most of the expression of cytokines/chemokines associated with pro-inflammation was severely decreased in taVNS with 15 Hz compared to 25 Hz. This study demonstrated that the taVNS parameter could differentially modulate the inflammation levels of animals, suggesting the importance of taVNS parameter selection for use in feasible interventions for acute inflammation treatment.https://www.mdpi.com/2227-9059/10/2/247transcutaneous auricular vagus nerve stimulation (taVNS)anti-inflammationcytokineselectrical stimulation parameterscoronavirus disease 2019 (COVID-19) |
| spellingShingle | Yoon-Young Go Won-Min Ju Chan-Mi Lee Sung-Won Chae Jae-Jun Song Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in Mice Biomedicines transcutaneous auricular vagus nerve stimulation (taVNS) anti-inflammation cytokines electrical stimulation parameters coronavirus disease 2019 (COVID-19) |
| title | Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in Mice |
| title_full | Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in Mice |
| title_fullStr | Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in Mice |
| title_full_unstemmed | Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in Mice |
| title_short | Different Transcutaneous Auricular Vagus Nerve Stimulation Parameters Modulate the Anti-Inflammatory Effects on Lipopolysaccharide-Induced Acute Inflammation in Mice |
| title_sort | different transcutaneous auricular vagus nerve stimulation parameters modulate the anti inflammatory effects on lipopolysaccharide induced acute inflammation in mice |
| topic | transcutaneous auricular vagus nerve stimulation (taVNS) anti-inflammation cytokines electrical stimulation parameters coronavirus disease 2019 (COVID-19) |
| url | https://www.mdpi.com/2227-9059/10/2/247 |
| work_keys_str_mv | AT yoonyounggo differenttranscutaneousauricularvagusnervestimulationparametersmodulatetheantiinflammatoryeffectsonlipopolysaccharideinducedacuteinflammationinmice AT wonminju differenttranscutaneousauricularvagusnervestimulationparametersmodulatetheantiinflammatoryeffectsonlipopolysaccharideinducedacuteinflammationinmice AT chanmilee differenttranscutaneousauricularvagusnervestimulationparametersmodulatetheantiinflammatoryeffectsonlipopolysaccharideinducedacuteinflammationinmice AT sungwonchae differenttranscutaneousauricularvagusnervestimulationparametersmodulatetheantiinflammatoryeffectsonlipopolysaccharideinducedacuteinflammationinmice AT jaejunsong differenttranscutaneousauricularvagusnervestimulationparametersmodulatetheantiinflammatoryeffectsonlipopolysaccharideinducedacuteinflammationinmice |