Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan
Background: Pomegranate (Punica granatum L.), one of the most important tropical fruits in Azad Jammu and Kashmir regions of Pakistan, is highly valued for its nutrition and medicinal purposes. Although pomegranate is native to this region, the genetic diversity among wild pomegranate accessions is...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345820300269 |
| _version_ | 1818850241485471744 |
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| author | Sadia Aziz Syeda Firdous Hifz Rahman Shahid Iqbal Awan Vincent Michael Geoffrey Meru |
| author_facet | Sadia Aziz Syeda Firdous Hifz Rahman Shahid Iqbal Awan Vincent Michael Geoffrey Meru |
| author_sort | Sadia Aziz |
| collection | DOAJ |
| description | Background: Pomegranate (Punica granatum L.), one of the most important tropical fruits in Azad Jammu and Kashmir regions of Pakistan, is highly valued for its nutrition and medicinal purposes. Although pomegranate is native to this region, the genetic diversity among wild pomegranate accessions is currently unknown. Such information would be vital for germplasm conservation and breeding efforts. In the current study, genetic diversity among forty-eight wild pomegranate accessions collected from different agro-ecological zones of Azad Jammu and Kashmir was assessed using 41 simple sequence repeat (SSR) markers. Results: The markers revealed 303 alleles averaging 7.39 alleles per marker. Polymorphic information content ranged from 0.12 (PGCT093B) to 0.88 (Pom006), with a mean of 0.54. The average genetic distance (GD) across all genotypes was 0.52, and was lowest between Chattar Class and Thorar genotypes (GD = 0.27), but highest between Khun Bandway and Akhor Ban (GD = 0.74). A neighbor-joining dendrogram separated the genotypes into three major clusters, with further sub-clustering within each cluster. Conclusions: Overall, the results presented here show significant genetic diversity among wild pomegranate accessions in Azad Jammu and Kashmir region of Pakistan. These accessions present a valuable genetic resource to breeding and cultivar improvement programs within the region.How to cite: Aziz S, Firdous S, Rahman H, et al. Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan. Electron J Biotechnol 2020;46. https://doi.org/10.1016/j.ejbt.2020.06.002. |
| first_indexed | 2024-12-19T06:46:00Z |
| format | Article |
| id | doaj.art-1361361cd8be483eb0715b0677bfaf3d |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-19T06:46:00Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-1361361cd8be483eb0715b0677bfaf3d2022-12-21T20:31:53ZengElsevierElectronic Journal of Biotechnology0717-34582020-07-01465054Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of PakistanSadia Aziz0Syeda Firdous1Hifz Rahman2Shahid Iqbal Awan3Vincent Michael4Geoffrey Meru5Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad 1300, PakistanDepartment of Botany, University of Azad Jammu and Kashmir, Muzaffarabad 1300, PakistanDepartment of Horticulture, University of Poonch, Rawalakot, Azad Kashmir, PakistanDepartment of Plant Breeding and Molecular Genetics, Faculty of Agriculture, University of Poonch, Rawalakot, Azad Kashmir, PakistanTropical Research and Education Center, Horticultural Sciences Department, University of Florida, Florida, USATropical Research and Education Center, Horticultural Sciences Department, University of Florida, Florida, USA; Corresponding author.Background: Pomegranate (Punica granatum L.), one of the most important tropical fruits in Azad Jammu and Kashmir regions of Pakistan, is highly valued for its nutrition and medicinal purposes. Although pomegranate is native to this region, the genetic diversity among wild pomegranate accessions is currently unknown. Such information would be vital for germplasm conservation and breeding efforts. In the current study, genetic diversity among forty-eight wild pomegranate accessions collected from different agro-ecological zones of Azad Jammu and Kashmir was assessed using 41 simple sequence repeat (SSR) markers. Results: The markers revealed 303 alleles averaging 7.39 alleles per marker. Polymorphic information content ranged from 0.12 (PGCT093B) to 0.88 (Pom006), with a mean of 0.54. The average genetic distance (GD) across all genotypes was 0.52, and was lowest between Chattar Class and Thorar genotypes (GD = 0.27), but highest between Khun Bandway and Akhor Ban (GD = 0.74). A neighbor-joining dendrogram separated the genotypes into three major clusters, with further sub-clustering within each cluster. Conclusions: Overall, the results presented here show significant genetic diversity among wild pomegranate accessions in Azad Jammu and Kashmir region of Pakistan. These accessions present a valuable genetic resource to breeding and cultivar improvement programs within the region.How to cite: Aziz S, Firdous S, Rahman H, et al. Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan. Electron J Biotechnol 2020;46. https://doi.org/10.1016/j.ejbt.2020.06.002.http://www.sciencedirect.com/science/article/pii/S0717345820300269AllelesDendrogramDNAGenetic distanceGenetic diversityPakistan |
| spellingShingle | Sadia Aziz Syeda Firdous Hifz Rahman Shahid Iqbal Awan Vincent Michael Geoffrey Meru Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan Electronic Journal of Biotechnology Alleles Dendrogram DNA Genetic distance Genetic diversity Pakistan |
| title | Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan |
| title_full | Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan |
| title_fullStr | Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan |
| title_full_unstemmed | Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan |
| title_short | Genetic diversity among wild pomegranate (Punica granatum) in Azad Jammu and Kashmir region of Pakistan |
| title_sort | genetic diversity among wild pomegranate punica granatum in azad jammu and kashmir region of pakistan |
| topic | Alleles Dendrogram DNA Genetic distance Genetic diversity Pakistan |
| url | http://www.sciencedirect.com/science/article/pii/S0717345820300269 |
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