Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics

Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics

Summary: The assembly of mitochondrial respiratory complexes into supercomplexes has significant implications for mitochondrial function. This protocol details mitochondrial isolation from mouse tissues and the use of blue native gel electrophoresis (BN-PAGE) to separate pre-identified mitochondrial...

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Bibliographic Details
Main Authors: Roger Moreno-Justicia, Alba Gonzalez-Franquesa, Ben Stocks, Atul S. Deshmukh
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Mass Spectrometry
Metabolism
Protein Biochemistry
Proteomics
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722000156

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