Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot study
<p>Abstract</p> <p>Background</p> <p>HIV-I can be transmitted by intravenous inoculation of contaminated blood or blood product or sexually through mucosal surfaces. Here we performed a pilot study in the SIV<sub>mac251</sub> macaque model to address whether...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2001-07-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/1/9 |
| _version_ | 1811286163897450496 |
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| author | Washington Parks Robyn Kelsall Brian Nacsa Janos Hel Zdenek Tryniszewska Elzbieta Stevceva Liljana Franchini Genoveffa |
| author_facet | Washington Parks Robyn Kelsall Brian Nacsa Janos Hel Zdenek Tryniszewska Elzbieta Stevceva Liljana Franchini Genoveffa |
| author_sort | Washington Parks Robyn |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>HIV-I can be transmitted by intravenous inoculation of contaminated blood or blood product or sexually through mucosal surfaces. Here we performed a pilot study in the SIV<sub>mac251</sub> macaque model to address whether the route of viral entry influences the kinetics of the appearance and the size of virus-specific immune in different tissue compartments.</p> <p>Methods</p> <p>For this purpose, of 2 genetically defined Mamu-A*01-positive macaques, 1 was exposed intravenously and the other intrarectally to the same SIV<sub>mac251</sub> viral stock and virus-specific CD8+ T-cells were measured within the first 12 days of infection in the blood and at day 12 in several tissues following euthanasia.</p> <p>Results</p> <p>Virus-specific CD8+ T-cell responses to Gag, Env, and particularly Tat appeared earlier in the blood of the animal exposed by the mucosal route than in the animal exposed intravenously. The magnitude of these virus-specific responses was consistently higher in the systemic tissues and GALT of the macaque exposed by the intravenous route, suggesting a higher viral burden in the tissues as reflected by the faster appearance of virus in plasma. Differences in the ability of the virus-specific CD8+ T-cells to respond <it>in vitro</it> to specific peptide stimulation were also observed and the greatest proliferative ability was found in the GALT of the animal infected by the intrarectal route.</p> <p>Conclusions</p> <p>These data may suggest that the natural mucosal barrier may delay viral spreading. The consequences of this observation, if confirmed in studies with a larger number of animals, may have implications in vaccine development.</p> |
| first_indexed | 2024-04-13T02:55:35Z |
| format | Article |
| id | doaj.art-1368713156fa4af89bdd54c6f0d4725e |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T02:55:35Z |
| publishDate | 2001-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-1368713156fa4af89bdd54c6f0d4725e2022-12-22T03:05:39ZengBMCBMC Infectious Diseases1471-23342001-07-0111910.1186/1471-2334-1-9Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot studyWashington Parks RobynKelsall BrianNacsa JanosHel ZdenekTryniszewska ElzbietaStevceva LiljanaFranchini Genoveffa<p>Abstract</p> <p>Background</p> <p>HIV-I can be transmitted by intravenous inoculation of contaminated blood or blood product or sexually through mucosal surfaces. Here we performed a pilot study in the SIV<sub>mac251</sub> macaque model to address whether the route of viral entry influences the kinetics of the appearance and the size of virus-specific immune in different tissue compartments.</p> <p>Methods</p> <p>For this purpose, of 2 genetically defined Mamu-A*01-positive macaques, 1 was exposed intravenously and the other intrarectally to the same SIV<sub>mac251</sub> viral stock and virus-specific CD8+ T-cells were measured within the first 12 days of infection in the blood and at day 12 in several tissues following euthanasia.</p> <p>Results</p> <p>Virus-specific CD8+ T-cell responses to Gag, Env, and particularly Tat appeared earlier in the blood of the animal exposed by the mucosal route than in the animal exposed intravenously. The magnitude of these virus-specific responses was consistently higher in the systemic tissues and GALT of the macaque exposed by the intravenous route, suggesting a higher viral burden in the tissues as reflected by the faster appearance of virus in plasma. Differences in the ability of the virus-specific CD8+ T-cells to respond <it>in vitro</it> to specific peptide stimulation were also observed and the greatest proliferative ability was found in the GALT of the animal infected by the intrarectal route.</p> <p>Conclusions</p> <p>These data may suggest that the natural mucosal barrier may delay viral spreading. The consequences of this observation, if confirmed in studies with a larger number of animals, may have implications in vaccine development.</p>http://www.biomedcentral.com/1471-2334/1/9 |
| spellingShingle | Washington Parks Robyn Kelsall Brian Nacsa Janos Hel Zdenek Tryniszewska Elzbieta Stevceva Liljana Franchini Genoveffa Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot study BMC Infectious Diseases |
| title | Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot study |
| title_full | Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot study |
| title_fullStr | Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot study |
| title_full_unstemmed | Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot study |
| title_short | Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIV<sub>mac251</sub> infection of rhesus macaques; a pilot study |
| title_sort | differences in time of virus appearance in the blood and virus specific immune responses in intravenous and intrarectal primary siv sub mac251 sub infection of rhesus macaques a pilot study |
| url | http://www.biomedcentral.com/1471-2334/1/9 |
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