Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq
Theileriosis is parasitic infection causes by obligate intracellular protozoa of the genus Theileria. T. lestoquardi is the most virulent species in sheep and goats which causes a severe disease with a high morbidity and mortality rate. In this study the phylogenetic relationships between two loca...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2016-12-01
|
| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://www.vetmedmosul.org/ijvs/media/16-2-2e.pdf |
| _version_ | 1828202162601066496 |
|---|---|
| author | M.J.A. Alkhaled N.N. A’aiz H.H. Naser |
| author_facet | M.J.A. Alkhaled N.N. A’aiz H.H. Naser |
| author_sort | M.J.A. Alkhaled |
| collection | DOAJ |
| description | Theileriosis is parasitic infection causes by obligate intracellular protozoa of the genus Theileria. T. lestoquardi is the most
virulent species in sheep and goats which causes a severe disease with a high morbidity and mortality rate. In this study the
phylogenetic relationships between two local isolate of T. lestoquardi and nine T. lestoquardi global isolates as well as
Babesia ovis out-group isolate were analyzed using the 18S rRNA gene sequence. The multiple sequence alignment analysis
and neighbor joining phylogenetic tree analysis were performed by using ClustalW multiple sequence alignment online based
analysis of 1098bp 18S rRNA gene was amplified by polymerase chain reaction. Phylogenetic analysis results of these gene
sequences revealed that T. lestoquardi local isolates were closely related to T. lestoquardi Iran isolate (JQ917458.1) and two
Iraq Kurdistan isolates (KC778786.1 and KC778785.1) more than other countries. This study represents the first report on the
use of molecular phylogeny to classify T. lestoquardi obtained in Middle Region of Iraq. |
| first_indexed | 2024-04-12T11:43:38Z |
| format | Article |
| id | doaj.art-136a776c3b374d83b4481310ceec09b1 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-12T11:43:38Z |
| publishDate | 2016-12-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-136a776c3b374d83b4481310ceec09b12022-12-22T03:34:30ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552016-12-0130 English22732Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of IraqM.J.A. Alkhaled0 N.N. A’aiz1 H.H. Naser 2 College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq Theileriosis is parasitic infection causes by obligate intracellular protozoa of the genus Theileria. T. lestoquardi is the most virulent species in sheep and goats which causes a severe disease with a high morbidity and mortality rate. In this study the phylogenetic relationships between two local isolate of T. lestoquardi and nine T. lestoquardi global isolates as well as Babesia ovis out-group isolate were analyzed using the 18S rRNA gene sequence. The multiple sequence alignment analysis and neighbor joining phylogenetic tree analysis were performed by using ClustalW multiple sequence alignment online based analysis of 1098bp 18S rRNA gene was amplified by polymerase chain reaction. Phylogenetic analysis results of these gene sequences revealed that T. lestoquardi local isolates were closely related to T. lestoquardi Iran isolate (JQ917458.1) and two Iraq Kurdistan isolates (KC778786.1 and KC778785.1) more than other countries. This study represents the first report on the use of molecular phylogeny to classify T. lestoquardi obtained in Middle Region of Iraq.http://www.vetmedmosul.org/ijvs/media/16-2-2e.pdfTheileriaPhylogenetic AnalysisOvinePCR18S rRNA gen |
| spellingShingle | M.J.A. Alkhaled N.N. A’aiz H.H. Naser Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq Iraqi Journal of Veterinary Sciences Theileria Phylogenetic Analysis Ovine PCR 18S rRNA gen |
| title | Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq |
| title_full | Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq |
| title_fullStr | Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq |
| title_full_unstemmed | Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq |
| title_short | Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq |
| title_sort | phylogenetic study of theileria lestoquardi based on 18srrna gene isolated from sheep in the middle region of iraq |
| topic | Theileria Phylogenetic Analysis Ovine PCR 18S rRNA gen |
| url | http://www.vetmedmosul.org/ijvs/media/16-2-2e.pdf |
| work_keys_str_mv | AT mjaalkhaled phylogeneticstudyoftheilerialestoquardibasedon18srrnageneisolatedfromsheepinthemiddleregionofiraq AT nnaaiz phylogeneticstudyoftheilerialestoquardibasedon18srrnageneisolatedfromsheepinthemiddleregionofiraq AT hhnaser phylogeneticstudyoftheilerialestoquardibasedon18srrnageneisolatedfromsheepinthemiddleregionofiraq |