Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus Cultures
<i>Solanum xanthocarpum</i> is considered an important traditional medicinal herb because of its unique antioxidant, and anti-diabetic, anti-aging, and anti-inflammatory potential. Because of the over exploitation linked to its medicinal properties as well as destruction of its natural h...
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MDPI AG
2020-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/9/2201 |
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| author | Hazrat Usman Muhammad Asad Ullah Hasnain Jan Aisha Siddiquah Samantha Drouet Sumaira Anjum Nathalie Giglioli-Guviarc’h Christophe Hano Bilal Haider Abbasi |
| author_facet | Hazrat Usman Muhammad Asad Ullah Hasnain Jan Aisha Siddiquah Samantha Drouet Sumaira Anjum Nathalie Giglioli-Guviarc’h Christophe Hano Bilal Haider Abbasi |
| author_sort | Hazrat Usman |
| collection | DOAJ |
| description | <i>Solanum xanthocarpum</i> is considered an important traditional medicinal herb because of its unique antioxidant, and anti-diabetic, anti-aging, and anti-inflammatory potential. Because of the over exploitation linked to its medicinal properties as well as destruction of its natural habitat, <i>S. xanthocarpum</i> is now becoming endangered and its supply is limited. Plant in vitro culture and elicitation are attractive alternative strategies to produce biomass and stimulate biosynthesis of medicinally important phytochemicals. Here, we investigated the potential influence of seven different monochromatic light treatments on biomass and secondary metabolites accumulation in callus culture of <i>S. xanthocarpum</i> as well as associated biological activities of the corresponding extracts. Among different light treatments, highest biomass accumulation was observed in white light-treated callus culture. Optimum accumulation of total flavonoid contents (TFC) and total phenolic contents (TPC) were observed in callus culture kept under continuous white and blue light respectively than control. Quantification of phytochemicals through HPLC revealed that optimum production of caffeic acid (0.57 ± 0.06 mg/g DW), methyl-caffeate (17.19 mg/g ± 1.79 DW), scopoletin (2.28 ± 0.13 mg/g DW), and esculetin (0.68 ± 0.07 mg/g DW) was observed under blue light callus cultures. Compared to the classic photoperiod condition, caffeic acid, methyl-caffeate, scopoletin, and esculetin were accumulated 1.7, 2.5, 1.1, and 1.09-folds higher, respectively. Moreover, high in vitro cell free antioxidant, anti-diabetic, anti-aging, and anti-inflammatory activities were closely associated with the production of these secondary metabolites. These results clearly showed the interest to apply multispectral light as elicitor of in vitro callus cultures <i>S. xanthocarpum</i> to promote the production of important phytochemicals, and allow us to propose this system as an alternative for the collection of this endangered species from the wild. |
| first_indexed | 2024-03-10T19:58:06Z |
| format | Article |
| id | doaj.art-136acbc66d1d4c11893e200e017345d4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:58:06Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-136acbc66d1d4c11893e200e017345d42023-11-19T23:48:17ZengMDPI AGMolecules1420-30492020-05-01259220110.3390/molecules25092201Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus CulturesHazrat Usman0Muhammad Asad Ullah1Hasnain Jan2Aisha Siddiquah3Samantha Drouet4Sumaira Anjum5Nathalie Giglioli-Guviarc’h6Christophe Hano7Bilal Haider Abbasi8Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad 45320, PakistanLaboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328 Unversité ď, CEDEX 2, 45067 Orléans, FranceDepartment of Biotechnology, Kinnaird College for Women, Lahore 54000, PakistanEA2106 Biomolecules et Biotechnologies Vegetales, Universite de Tours, 37000 Tours, FranceLaboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328 Unversité ď, CEDEX 2, 45067 Orléans, FranceDepartment of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan<i>Solanum xanthocarpum</i> is considered an important traditional medicinal herb because of its unique antioxidant, and anti-diabetic, anti-aging, and anti-inflammatory potential. Because of the over exploitation linked to its medicinal properties as well as destruction of its natural habitat, <i>S. xanthocarpum</i> is now becoming endangered and its supply is limited. Plant in vitro culture and elicitation are attractive alternative strategies to produce biomass and stimulate biosynthesis of medicinally important phytochemicals. Here, we investigated the potential influence of seven different monochromatic light treatments on biomass and secondary metabolites accumulation in callus culture of <i>S. xanthocarpum</i> as well as associated biological activities of the corresponding extracts. Among different light treatments, highest biomass accumulation was observed in white light-treated callus culture. Optimum accumulation of total flavonoid contents (TFC) and total phenolic contents (TPC) were observed in callus culture kept under continuous white and blue light respectively than control. Quantification of phytochemicals through HPLC revealed that optimum production of caffeic acid (0.57 ± 0.06 mg/g DW), methyl-caffeate (17.19 mg/g ± 1.79 DW), scopoletin (2.28 ± 0.13 mg/g DW), and esculetin (0.68 ± 0.07 mg/g DW) was observed under blue light callus cultures. Compared to the classic photoperiod condition, caffeic acid, methyl-caffeate, scopoletin, and esculetin were accumulated 1.7, 2.5, 1.1, and 1.09-folds higher, respectively. Moreover, high in vitro cell free antioxidant, anti-diabetic, anti-aging, and anti-inflammatory activities were closely associated with the production of these secondary metabolites. These results clearly showed the interest to apply multispectral light as elicitor of in vitro callus cultures <i>S. xanthocarpum</i> to promote the production of important phytochemicals, and allow us to propose this system as an alternative for the collection of this endangered species from the wild.https://www.mdpi.com/1420-3049/25/9/2201<i>Solanum xanthocarpum</i>elicitationmultispectral lightscaffeic acidcoumarinsanti-diabetic |
| spellingShingle | Hazrat Usman Muhammad Asad Ullah Hasnain Jan Aisha Siddiquah Samantha Drouet Sumaira Anjum Nathalie Giglioli-Guviarc’h Christophe Hano Bilal Haider Abbasi Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus Cultures Molecules <i>Solanum xanthocarpum</i> elicitation multispectral lights caffeic acid coumarins anti-diabetic |
| title | Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus Cultures |
| title_full | Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus Cultures |
| title_fullStr | Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus Cultures |
| title_full_unstemmed | Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus Cultures |
| title_short | Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of <i>Solanum xanthocarpum</i> Callus Cultures |
| title_sort | interactive effects of wide spectrum monochromatic lights on phytochemical production antioxidant and biological activities of i solanum xanthocarpum i callus cultures |
| topic | <i>Solanum xanthocarpum</i> elicitation multispectral lights caffeic acid coumarins anti-diabetic |
| url | https://www.mdpi.com/1420-3049/25/9/2201 |
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