Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp
Background/Aim. Proinflammatory cytokines can act like endogenous pyrogen interleukin 1 (IL-1), interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF α) which regulate the synthesis of secondary mediators and other proinflammatory cytokines through macrophages and mesenchymal cells. They stimul...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2017-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2017/0042-84501600172S.pdf |
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| author | Šubarić Ljiljana Mitić Aleksandar Matvijenko Vladimir Jovanović Radovan Živković Dušan Perić Dejan Vlahović Zoran |
| author_facet | Šubarić Ljiljana Mitić Aleksandar Matvijenko Vladimir Jovanović Radovan Živković Dušan Perić Dejan Vlahović Zoran |
| author_sort | Šubarić Ljiljana |
| collection | DOAJ |
| description | Background/Aim. Proinflammatory cytokines can act like endogenous pyrogen interleukin 1 (IL-1), interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF α) which regulate the synthesis of secondary mediators and other proinflammatory cytokines through macrophages and mesenchymal cells. They stimulate acute-phase proteins and attract inflammatory cells. The aim of this study was to determine interleukin 1-β (IL-1 β) concentrations in chronically inflamed and healthy dental pulps. Methods. A total of 41 pulps (19 from patients with pulpitis chronic causa and 22 from patients with pulpatis chronic aperta), divided into two groups, were obtained from teeth with chronic pulp inflammation. The control group consisted of 12 teeth with healthy pulp. After extirpation, pulp samples were immediately placed in sterile Eppendorf tubes and frozen. After that, homogenisation was performed by a Teflon® pestle in ice-cold phosphate buffer solution at pH 7.4 whose volume was adjusted according to the weight of tissue. The supernatant was then frozen at -70°C until the performance of appropriate biochemical analyses. Cytokine IL-1 β value was determined by a commercial enzyme- linked immunosorbent assay (ELISA test). We applied the high sensitivity system technique, which may register low levels of cytokines, ranging from 0.125 to 8.0 pg/mL for IL-1 β. Results. By comparing the mean value of IL-1β, in the pulps we can see a statistically significant difference (p < 0.01) among them. The highest value of IL-1 β was in the subjects with pulpitis chronica clausa and it was 6.21 ± 2.70 pg/mL. Conclusion. Proinflammatory cytokine IL-1 β is present in detectable quantities in the pulp tissue of all vital pulps. Its highest concentrations were found in the sample group with pulpitis chronica clausa. |
| first_indexed | 2024-04-12T01:21:36Z |
| format | Article |
| id | doaj.art-136b2831f1024fe78713f566e225679a |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2024-04-12T01:21:36Z |
| publishDate | 2017-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-136b2831f1024fe78713f566e225679a2022-12-22T03:53:47ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502406-07202017-01-0174325626010.2298/VSP140303172S0042-84501600172SInterleukin 1-beta analysis in chronically inflamed and healthy human dental pulpŠubarić Ljiljana0Mitić Aleksandar1Matvijenko Vladimir2Jovanović Radovan3Živković Dušan4Perić Dejan5Vlahović Zoran6Clinic of Dental Medicine, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, Kosovska MitrovicaClinic of Dental Medicine, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, NišClinic of Dental Medicine, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, Kosovska MitrovicaClinic of Dental Medicine, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, Kosovska MitrovicaClinic of Dental Medicine, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, Kosovska MitrovicaClinic of Dental Medicine, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, Kosovska MitrovicaClinic of Dental Medicine, Faculty of Medicine, Department of Oral Surgery, Kosovska MitrovicaBackground/Aim. Proinflammatory cytokines can act like endogenous pyrogen interleukin 1 (IL-1), interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF α) which regulate the synthesis of secondary mediators and other proinflammatory cytokines through macrophages and mesenchymal cells. They stimulate acute-phase proteins and attract inflammatory cells. The aim of this study was to determine interleukin 1-β (IL-1 β) concentrations in chronically inflamed and healthy dental pulps. Methods. A total of 41 pulps (19 from patients with pulpitis chronic causa and 22 from patients with pulpatis chronic aperta), divided into two groups, were obtained from teeth with chronic pulp inflammation. The control group consisted of 12 teeth with healthy pulp. After extirpation, pulp samples were immediately placed in sterile Eppendorf tubes and frozen. After that, homogenisation was performed by a Teflon® pestle in ice-cold phosphate buffer solution at pH 7.4 whose volume was adjusted according to the weight of tissue. The supernatant was then frozen at -70°C until the performance of appropriate biochemical analyses. Cytokine IL-1 β value was determined by a commercial enzyme- linked immunosorbent assay (ELISA test). We applied the high sensitivity system technique, which may register low levels of cytokines, ranging from 0.125 to 8.0 pg/mL for IL-1 β. Results. By comparing the mean value of IL-1β, in the pulps we can see a statistically significant difference (p < 0.01) among them. The highest value of IL-1 β was in the subjects with pulpitis chronica clausa and it was 6.21 ± 2.70 pg/mL. Conclusion. Proinflammatory cytokine IL-1 β is present in detectable quantities in the pulp tissue of all vital pulps. Its highest concentrations were found in the sample group with pulpitis chronica clausa.http://www.doiserbia.nb.rs/img/doi/0042-8450/2017/0042-84501600172S.pdfdental pulp diseaseschronic diseaseinflammationinterleukin-1 beta |
| spellingShingle | Šubarić Ljiljana Mitić Aleksandar Matvijenko Vladimir Jovanović Radovan Živković Dušan Perić Dejan Vlahović Zoran Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp Vojnosanitetski Pregled dental pulp diseases chronic disease inflammation interleukin-1 beta |
| title | Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp |
| title_full | Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp |
| title_fullStr | Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp |
| title_full_unstemmed | Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp |
| title_short | Interleukin 1-beta analysis in chronically inflamed and healthy human dental pulp |
| title_sort | interleukin 1 beta analysis in chronically inflamed and healthy human dental pulp |
| topic | dental pulp diseases chronic disease inflammation interleukin-1 beta |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2017/0042-84501600172S.pdf |
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