Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012
<i>Streptococcus agalactiae</i> (GBS) can infect newborns, pregnant women and immunocompromised or elderly people. This study aimed to investigate differences in three pilus genes and virulence genes <i>pavA</i>, <i>cfb</i>, <i>rib</i> and <i>scp...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/11/10/1145 |
| _version_ | 1797470503654391808 |
|---|---|
| author | I-An Tsai Yaochi Su Ying-Hsiang Wang Chishih Chu |
| author_facet | I-An Tsai Yaochi Su Ying-Hsiang Wang Chishih Chu |
| author_sort | I-An Tsai |
| collection | DOAJ |
| description | <i>Streptococcus agalactiae</i> (GBS) can infect newborns, pregnant women and immunocompromised or elderly people. This study aimed to investigate differences in three pilus genes and virulence genes <i>pavA</i>, <i>cfb</i>, <i>rib</i> and <i>scpB</i> and changes in predominant serotypes III, V and VI from 2008 to 2012. The susceptibilities to penicillin, ceftriaxone, azithromycin, erythromycin, clindamycin, levofloxacin and moxifloxacin of 145 GBS strains of serotype III, V and VI strains from 2008 and 2012 were determined using disc diffusion method. PCR identification of ST-17, the pilus genes and virulence genes; multilocus sequence typing (MLST); and conserved domain and phylogenetic analysis of <i>scpB-1</i> and <i>scpB-2</i> proteins were performed. A dramatic number reduction was observed in serotype V, not III and V, from 2008 to 2012. The rate of resistance to azithromycin, clindamycin and erythromycin was the highest in serotype V. ST-17 was only found in serotype III with pilus genes PI-1+PI-2b. The major pilus genotype was PI-1+PI-2a. Serotype V without the <i>rib</i> gene was reduced in number between two studied years. Compared to <i>scpB-1</i>, <i>scpB-2</i> had a 128-bp deletion in a PA C5a-like peptidase domain and putative integrin-binding motif RGD. In conclusion, reduction in serotype V may be due to presence of <i>scpB-2</i> or lack of genes <i>scpB</i> and <i>rib</i>. |
| first_indexed | 2024-03-09T19:37:12Z |
| format | Article |
| id | doaj.art-136d3b796ef04aa0b8d77ef1a26ffc67 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T19:37:12Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-136d3b796ef04aa0b8d77ef1a26ffc672023-11-24T01:48:40ZengMDPI AGPathogens2076-08172022-10-011110114510.3390/pathogens11101145Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012I-An Tsai0Yaochi Su1Ying-Hsiang Wang2Chishih Chu3Ph.D. Program of Agriculture Science, College of Agriculture, National Chiayi University, Chiayi City 600, TaiwanPh.D. Program of Agriculture Science, College of Agriculture, National Chiayi University, Chiayi City 600, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital, Puzi City 613, TaiwanDepartment of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi City 600, Taiwan<i>Streptococcus agalactiae</i> (GBS) can infect newborns, pregnant women and immunocompromised or elderly people. This study aimed to investigate differences in three pilus genes and virulence genes <i>pavA</i>, <i>cfb</i>, <i>rib</i> and <i>scpB</i> and changes in predominant serotypes III, V and VI from 2008 to 2012. The susceptibilities to penicillin, ceftriaxone, azithromycin, erythromycin, clindamycin, levofloxacin and moxifloxacin of 145 GBS strains of serotype III, V and VI strains from 2008 and 2012 were determined using disc diffusion method. PCR identification of ST-17, the pilus genes and virulence genes; multilocus sequence typing (MLST); and conserved domain and phylogenetic analysis of <i>scpB-1</i> and <i>scpB-2</i> proteins were performed. A dramatic number reduction was observed in serotype V, not III and V, from 2008 to 2012. The rate of resistance to azithromycin, clindamycin and erythromycin was the highest in serotype V. ST-17 was only found in serotype III with pilus genes PI-1+PI-2b. The major pilus genotype was PI-1+PI-2a. Serotype V without the <i>rib</i> gene was reduced in number between two studied years. Compared to <i>scpB-1</i>, <i>scpB-2</i> had a 128-bp deletion in a PA C5a-like peptidase domain and putative integrin-binding motif RGD. In conclusion, reduction in serotype V may be due to presence of <i>scpB-2</i> or lack of genes <i>scpB</i> and <i>rib</i>.https://www.mdpi.com/2076-0817/11/10/1145<i>Streptococcus agalactiae</i>antimicrobial susceptibilitypilus island genesvirulence genes<i>scpB</i> gene<i>rib</i> gene |
| spellingShingle | I-An Tsai Yaochi Su Ying-Hsiang Wang Chishih Chu Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012 Pathogens <i>Streptococcus agalactiae</i> antimicrobial susceptibility pilus island genes virulence genes <i>scpB</i> gene <i>rib</i> gene |
| title | Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012 |
| title_full | Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012 |
| title_fullStr | Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012 |
| title_full_unstemmed | Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012 |
| title_short | Alterations in Genes <em>rib</em>, <em>scpB</em> and Pilus Island Decrease the Prevalence of Predominant Serotype V, Not III and VI, of <em>Streptococcus agalactiae</em> from 2008 to 2012 |
| title_sort | alterations in genes em rib em em scpb em and pilus island decrease the prevalence of predominant serotype v not iii and vi of em streptococcus agalactiae em from 2008 to 2012 |
| topic | <i>Streptococcus agalactiae</i> antimicrobial susceptibility pilus island genes virulence genes <i>scpB</i> gene <i>rib</i> gene |
| url | https://www.mdpi.com/2076-0817/11/10/1145 |
| work_keys_str_mv | AT iantsai alterationsingenesemribememscpbemandpilusislanddecreasetheprevalenceofpredominantserotypevnotiiiandviofemstreptococcusagalactiaeemfrom2008to2012 AT yaochisu alterationsingenesemribememscpbemandpilusislanddecreasetheprevalenceofpredominantserotypevnotiiiandviofemstreptococcusagalactiaeemfrom2008to2012 AT yinghsiangwang alterationsingenesemribememscpbemandpilusislanddecreasetheprevalenceofpredominantserotypevnotiiiandviofemstreptococcusagalactiaeemfrom2008to2012 AT chishihchu alterationsingenesemribememscpbemandpilusislanddecreasetheprevalenceofpredominantserotypevnotiiiandviofemstreptococcusagalactiaeemfrom2008to2012 |