Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers
Abstract Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techn...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45575-5 |
| _version_ | 1797636864471990272 |
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| author | Dominik Drabik Mitja Drab Samo Penič Aleš Iglič Aleksander Czogalla |
| author_facet | Dominik Drabik Mitja Drab Samo Penič Aleš Iglič Aleksander Czogalla |
| author_sort | Dominik Drabik |
| collection | DOAJ |
| description | Abstract Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present significant challenges. In this study we employ spot-variation z-scan fluorescence correlation spectroscopy (svzFCS) tailored for artificial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of definitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies—including the analysis of diffusion coefficient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confirming that nano- and microdomains can co-exist in lipid vesicles. |
| first_indexed | 2024-03-11T12:41:23Z |
| format | Article |
| id | doaj.art-13721288255846af9a19d5f7470612d3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T12:41:23Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-13721288255846af9a19d5f7470612d32023-11-05T12:17:13ZengNature PortfolioScientific Reports2045-23222023-10-0113111410.1038/s41598-023-45575-5Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayersDominik Drabik0Mitja Drab1Samo Penič2Aleš Iglič3Aleksander Czogalla4Laboratory of Cytobiochemistry, Faculty of Biotechnology, University of WroclawLaboratory of Physics, Faculty of Electrical Engineering, University of LjubljanaLaboratory of Physics, Faculty of Electrical Engineering, University of LjubljanaLaboratory of Physics, Faculty of Electrical Engineering, University of LjubljanaLaboratory of Cytobiochemistry, Faculty of Biotechnology, University of WroclawAbstract Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present significant challenges. In this study we employ spot-variation z-scan fluorescence correlation spectroscopy (svzFCS) tailored for artificial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of definitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies—including the analysis of diffusion coefficient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confirming that nano- and microdomains can co-exist in lipid vesicles.https://doi.org/10.1038/s41598-023-45575-5 |
| spellingShingle | Dominik Drabik Mitja Drab Samo Penič Aleš Iglič Aleksander Czogalla Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers Scientific Reports |
| title | Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers |
| title_full | Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers |
| title_fullStr | Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers |
| title_full_unstemmed | Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers |
| title_short | Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers |
| title_sort | investigation of nano and microdomains formed by ceramide 1 phosphate in lipid bilayers |
| url | https://doi.org/10.1038/s41598-023-45575-5 |
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