Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.)
This study featured broad/fava bean pods as by-products of food production. It assessed the chemical composition of green bean pods (Vicia faba L.) and their methanolic extract. The extract was tested in vitro for antioxidant, anti-inflammatory, antimicrobial, and anticancer activities against prost...
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2024-02-01
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| Series: | Foods and Raw Materials |
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| Online Access: | https://jfrm.ru/en/issues/22111/22168/ |
| _version_ | 1797345830896664576 |
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| author | Elsayed Elbadrawy Mona Y. Mostafa |
| author_facet | Elsayed Elbadrawy Mona Y. Mostafa |
| author_sort | Elsayed Elbadrawy |
| collection | DOAJ |
| description | This study featured broad/fava bean pods as by-products of food production. It assessed the chemical composition of green bean pods (Vicia faba L.) and their methanolic extract. The extract was tested in vitro for antioxidant, anti-inflammatory, antimicrobial, and anticancer activities against prostate cancer (Pc3) and liver cancer (HepG2) cells. Broad bean pods proved to be rich in carbohydrates, fiber, protein, potassium, calcium, and magnesium. The extract contained 286 mg GAE/g total phenols and 105 mg QE/g total flavonoids. The antioxidant activity of the methanolic extract was measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay. The highest DPPH scavenging activity belonged to the extract concentrations of 1000 µg/mL (80.5%) and 500 µg/mL (73.7%), whereas the IC50 value was 87.35 µg/mL. The methanolic extract possessed the anti-inflammatory effect as it significantly reduced the hemolysis of red blood cells. The maximal inhibition percentage reached 66.7% at 1000 µg/mL. Regarding the antimicrobial activity, the broad bean pod metha- nolic extract inhibited Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, as well as Candida albicans. The extract reduced the cell viability of human hepatocarcinoma (HepG2) and prostate cancer (PC3) cells in a concentration-dependent manner. It also caused significant changes in cell shape, compared to the control. Therefore, broad beans can be recommended for human consumption together with pods, fresh or cooked, as a potential source of bioactive substances in functional food production. |
| first_indexed | 2024-03-08T11:23:23Z |
| format | Article |
| id | doaj.art-1373e913ba514ffab6cd4a0d9f5827e4 |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-03-08T11:23:23Z |
| publishDate | 2024-02-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-1373e913ba514ffab6cd4a0d9f5827e42024-01-26T05:38:46ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992024-02-0112230831810.21603/2308-4057-2024-2-610Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.)Elsayed Elbadrawy0https://orcid.org/0000-0001-7279-7942Mona Y. Mostafa1https://orcid.org/0000-0002-8203-2596Mansoura University , Mansoura, EgyptMansoura University , Mansoura, EgyptThis study featured broad/fava bean pods as by-products of food production. It assessed the chemical composition of green bean pods (Vicia faba L.) and their methanolic extract. The extract was tested in vitro for antioxidant, anti-inflammatory, antimicrobial, and anticancer activities against prostate cancer (Pc3) and liver cancer (HepG2) cells. Broad bean pods proved to be rich in carbohydrates, fiber, protein, potassium, calcium, and magnesium. The extract contained 286 mg GAE/g total phenols and 105 mg QE/g total flavonoids. The antioxidant activity of the methanolic extract was measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay. The highest DPPH scavenging activity belonged to the extract concentrations of 1000 µg/mL (80.5%) and 500 µg/mL (73.7%), whereas the IC50 value was 87.35 µg/mL. The methanolic extract possessed the anti-inflammatory effect as it significantly reduced the hemolysis of red blood cells. The maximal inhibition percentage reached 66.7% at 1000 µg/mL. Regarding the antimicrobial activity, the broad bean pod metha- nolic extract inhibited Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, as well as Candida albicans. The extract reduced the cell viability of human hepatocarcinoma (HepG2) and prostate cancer (PC3) cells in a concentration-dependent manner. It also caused significant changes in cell shape, compared to the control. Therefore, broad beans can be recommended for human consumption together with pods, fresh or cooked, as a potential source of bioactive substances in functional food production.https://jfrm.ru/en/issues/22111/22168/vicia faba l. podsanticancer effectantioxidant activityanti-inflammatory propertiesantimicrobial abilitydpph radicals |
| spellingShingle | Elsayed Elbadrawy Mona Y. Mostafa Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.) Foods and Raw Materials vicia faba l. pods anticancer effect antioxidant activity anti-inflammatory properties antimicrobial ability dpph radicals |
| title | Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.) |
| title_full | Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.) |
| title_fullStr | Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.) |
| title_full_unstemmed | Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.) |
| title_short | Antioxidant, anti-inflammatory, antimicrobial, and anticancer properties of green broad bean pods (Vicia faba L.) |
| title_sort | antioxidant anti inflammatory antimicrobial and anticancer properties of green broad bean pods vicia faba l |
| topic | vicia faba l. pods anticancer effect antioxidant activity anti-inflammatory properties antimicrobial ability dpph radicals |
| url | https://jfrm.ru/en/issues/22111/22168/ |
| work_keys_str_mv | AT elsayedelbadrawy antioxidantantiinflammatoryantimicrobialandanticancerpropertiesofgreenbroadbeanpodsviciafabal AT monaymostafa antioxidantantiinflammatoryantimicrobialandanticancerpropertiesofgreenbroadbeanpodsviciafabal |