Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration.
Fidelity in pluripotent stem cell differentiation protocols is necessary for the therapeutic and commercial use of cells derived from embryonic and induced pluripotent stem cells. Recent advances in stem cell technology, especially the widespread availability of a range of chemically defined media,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5345835?pdf=render |
| _version_ | 1819163659457265664 |
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| author | Zhouhui Geng Patrick J Walsh Vincent Truong Caitlin Hill Mara Ebeling Rebecca J Kapphahn Sandra R Montezuma Ching Yuan Heidi Roehrich Deborah A Ferrington James R Dutton |
| author_facet | Zhouhui Geng Patrick J Walsh Vincent Truong Caitlin Hill Mara Ebeling Rebecca J Kapphahn Sandra R Montezuma Ching Yuan Heidi Roehrich Deborah A Ferrington James R Dutton |
| author_sort | Zhouhui Geng |
| collection | DOAJ |
| description | Fidelity in pluripotent stem cell differentiation protocols is necessary for the therapeutic and commercial use of cells derived from embryonic and induced pluripotent stem cells. Recent advances in stem cell technology, especially the widespread availability of a range of chemically defined media, substrates and differentiation components, now allow the design and implementation of fully defined derivation and differentiation protocols intended for replication across multiple research and manufacturing locations. In this report we present an application of these criteria to the generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. Primary conjunctival cells from human donors aged 70-85 years were reprogrammed to derive multiple iPSC lines that were differentiated into functional RPE using a rapid and defined differentiation protocol. The combination of defined iPSC derivation and culture with a defined RPE differentiation protocol, reproducibly generated functional RPE from each donor without requiring protocol adjustments for each individual. This successful validation of a standardized, iPSC derivation and RPE differentiation process demonstrates a practical approach for applications requiring the cost-effective generation of RPE from multiple individuals such as drug testing, population studies or for therapies requiring patient-specific RPE derivations. In addition, conjunctival cells are identified as a practical source of somatic cells for deriving iPSCs from elderly individuals. |
| first_indexed | 2024-12-22T17:47:39Z |
| format | Article |
| id | doaj.art-13792230b270489392ea6ccef6085339 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:47:39Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-13792230b270489392ea6ccef60853392022-12-21T18:18:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017357510.1371/journal.pone.0173575Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration.Zhouhui GengPatrick J WalshVincent TruongCaitlin HillMara EbelingRebecca J KapphahnSandra R MontezumaChing YuanHeidi RoehrichDeborah A FerringtonJames R DuttonFidelity in pluripotent stem cell differentiation protocols is necessary for the therapeutic and commercial use of cells derived from embryonic and induced pluripotent stem cells. Recent advances in stem cell technology, especially the widespread availability of a range of chemically defined media, substrates and differentiation components, now allow the design and implementation of fully defined derivation and differentiation protocols intended for replication across multiple research and manufacturing locations. In this report we present an application of these criteria to the generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. Primary conjunctival cells from human donors aged 70-85 years were reprogrammed to derive multiple iPSC lines that were differentiated into functional RPE using a rapid and defined differentiation protocol. The combination of defined iPSC derivation and culture with a defined RPE differentiation protocol, reproducibly generated functional RPE from each donor without requiring protocol adjustments for each individual. This successful validation of a standardized, iPSC derivation and RPE differentiation process demonstrates a practical approach for applications requiring the cost-effective generation of RPE from multiple individuals such as drug testing, population studies or for therapies requiring patient-specific RPE derivations. In addition, conjunctival cells are identified as a practical source of somatic cells for deriving iPSCs from elderly individuals.http://europepmc.org/articles/PMC5345835?pdf=render |
| spellingShingle | Zhouhui Geng Patrick J Walsh Vincent Truong Caitlin Hill Mara Ebeling Rebecca J Kapphahn Sandra R Montezuma Ching Yuan Heidi Roehrich Deborah A Ferrington James R Dutton Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE |
| title | Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. |
| title_full | Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. |
| title_fullStr | Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. |
| title_full_unstemmed | Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. |
| title_short | Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. |
| title_sort | generation of retinal pigmented epithelium from ipscs derived from the conjunctiva of donors with and without age related macular degeneration |
| url | http://europepmc.org/articles/PMC5345835?pdf=render |
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