A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises
Genome editing (GE) has revolutionized the biological sciences by creating a novel approach for manipulating the genomes of living organisms. Many tools have been developed in recent years to enable the editing of complex genomes. Therefore, a reliable and rapid approach for increasing yield and tol...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/43/3/135 |
| _version_ | 1797505802427170816 |
|---|---|
| author | Adnan Rasheed Rafaqat Ali Gill Muhammad Umair Hassan Athar Mahmood Sameer Qari Qamar U. Zaman Muhammad Ilyas Muhammad Aamer Maria Batool Huijie Li Ziming Wu |
| author_facet | Adnan Rasheed Rafaqat Ali Gill Muhammad Umair Hassan Athar Mahmood Sameer Qari Qamar U. Zaman Muhammad Ilyas Muhammad Aamer Maria Batool Huijie Li Ziming Wu |
| author_sort | Adnan Rasheed |
| collection | DOAJ |
| description | Genome editing (GE) has revolutionized the biological sciences by creating a novel approach for manipulating the genomes of living organisms. Many tools have been developed in recent years to enable the editing of complex genomes. Therefore, a reliable and rapid approach for increasing yield and tolerance to various environmental stresses is necessary to sustain agricultural crop production for global food security. This critical review elaborates the GE tools used for crop improvement. These tools include mega-nucleases (MNs), such as zinc-finger nucleases (ZFNs), and transcriptional activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, this review addresses the latest advancements in the role of CRISPR/Cas9 for genome manipulation for major crop improvement, including yield and quality development of biotic stress- and abiotic stress-tolerant crops. Implementation of this technique will lead to the production of non-transgene crops with preferred characteristics that can result in enhanced yield capacity under various environmental stresses. The CRISPR/Cas9 technique can be combined with current and potential breeding methods (e.g., speed breeding and omics-assisted breeding) to enhance agricultural productivity to ensure food security. We have also discussed the challenges and limitations of CRISPR/Cas9. This information will be useful to plant breeders and researchers in the thorough investigation of the use of CRISPR/Cas9 to boost crops by targeting the gene of interest. |
| first_indexed | 2024-03-10T04:23:32Z |
| format | Article |
| id | doaj.art-137bb6b463c84225a091d5232de4de81 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T04:23:32Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-137bb6b463c84225a091d5232de4de812023-11-23T07:44:10ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452021-11-014331950197610.3390/cimb43030135A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food CrisesAdnan Rasheed0Rafaqat Ali Gill1Muhammad Umair Hassan2Athar Mahmood3Sameer Qari4Qamar U. Zaman5Muhammad Ilyas6Muhammad Aamer7Maria Batool8Huijie Li9Ziming Wu10Key Laboratory of Crops Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaResearch Center on Ecological Sciences, Jiangxi Agricultural University, Nanchang 330045, ChinaDepartment of Agronomy, University of Agriculture, Faisalabad 38040, PakistanBiology Department, (Genetics and Molecular Biology Central Laboratory), Aljumum University College, Umm Al-Qura University, Makkah 24382, Saudi ArabiaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaUniversity College of Dera Murad Jamali, Nasirabad 80700, Balochistan, PakistanResearch Center on Ecological Sciences, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crops Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crops Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaGenome editing (GE) has revolutionized the biological sciences by creating a novel approach for manipulating the genomes of living organisms. Many tools have been developed in recent years to enable the editing of complex genomes. Therefore, a reliable and rapid approach for increasing yield and tolerance to various environmental stresses is necessary to sustain agricultural crop production for global food security. This critical review elaborates the GE tools used for crop improvement. These tools include mega-nucleases (MNs), such as zinc-finger nucleases (ZFNs), and transcriptional activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, this review addresses the latest advancements in the role of CRISPR/Cas9 for genome manipulation for major crop improvement, including yield and quality development of biotic stress- and abiotic stress-tolerant crops. Implementation of this technique will lead to the production of non-transgene crops with preferred characteristics that can result in enhanced yield capacity under various environmental stresses. The CRISPR/Cas9 technique can be combined with current and potential breeding methods (e.g., speed breeding and omics-assisted breeding) to enhance agricultural productivity to ensure food security. We have also discussed the challenges and limitations of CRISPR/Cas9. This information will be useful to plant breeders and researchers in the thorough investigation of the use of CRISPR/Cas9 to boost crops by targeting the gene of interest.https://www.mdpi.com/1467-3045/43/3/135CRISPR/Cas9cropsyieldbioticabiotic stresses |
| spellingShingle | Adnan Rasheed Rafaqat Ali Gill Muhammad Umair Hassan Athar Mahmood Sameer Qari Qamar U. Zaman Muhammad Ilyas Muhammad Aamer Maria Batool Huijie Li Ziming Wu A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises Current Issues in Molecular Biology CRISPR/Cas9 crops yield biotic abiotic stresses |
| title | A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises |
| title_full | A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises |
| title_fullStr | A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises |
| title_full_unstemmed | A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises |
| title_short | A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises |
| title_sort | critical review recent advancements in the use of crispr cas9 technology to enhance crops and alleviate global food crises |
| topic | CRISPR/Cas9 crops yield biotic abiotic stresses |
| url | https://www.mdpi.com/1467-3045/43/3/135 |
| work_keys_str_mv | AT adnanrasheed acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT rafaqataligill acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT muhammadumairhassan acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT atharmahmood acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT sameerqari acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT qamaruzaman acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT muhammadilyas acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT muhammadaamer acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT mariabatool acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT huijieli acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT zimingwu acriticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT adnanrasheed criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT rafaqataligill criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT muhammadumairhassan criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT atharmahmood criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT sameerqari criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT qamaruzaman criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT muhammadilyas criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT muhammadaamer criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT mariabatool criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT huijieli criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises AT zimingwu criticalreviewrecentadvancementsintheuseofcrisprcas9technologytoenhancecropsandalleviateglobalfoodcrises |