The effect of caffeine supplementation on exercise performance evaluated by a novel animal model
Introduction: Caffeinated drinks are used for improve performance. Animal models represent investigational strategy that circumvents most of the drawbacks of research in humans, including motivational factors and the placebo effect. No animal model that could test whether different forms of administ...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS)
2017-12-01
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| Series: | Clinical and Biomedical Research |
| Subjects: | |
| Online Access: | http://seer.ufrgs.br/index.php/hcpa/article/view/74773 |
| _version_ | 1819135832819236864 |
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| author | Roberto Pacheco da Silva Denis Martinez Cintia Zappe Fiori Kelly Silveira da Silva Bueno Jhoana Mercedes Uribe Ramos Renata Schenkel Kaminski Marcia Kraide Fischer Leticia Maria Tedesco Silva Juliana Neves Giordani Juliana Heitich Brendler Juliana Langendorf da Costa Vieira Yasmim de Freitas Dias Laura Martinewski de Oliveira Chaiane Facco Piccin Emerson Ferreira Martins |
| author_facet | Roberto Pacheco da Silva Denis Martinez Cintia Zappe Fiori Kelly Silveira da Silva Bueno Jhoana Mercedes Uribe Ramos Renata Schenkel Kaminski Marcia Kraide Fischer Leticia Maria Tedesco Silva Juliana Neves Giordani Juliana Heitich Brendler Juliana Langendorf da Costa Vieira Yasmim de Freitas Dias Laura Martinewski de Oliveira Chaiane Facco Piccin Emerson Ferreira Martins |
| author_sort | Roberto Pacheco da Silva |
| collection | DOAJ |
| description | Introduction: Caffeinated drinks are used for improve performance. Animal models represent investigational strategy that circumvents most of the drawbacks of research in humans, including motivational factors and the placebo effect. No animal model that could test whether different forms of administering caffeine affect exercise propensity was found in the literature.
Methods: An animal model of grouped voluntary exercise was tested. Two-month-old male C57/bl mice were housed in a cage fitted with one running wheel and a monitoring system. Six animals per cage were introduced individually. To assess the sensitivity of the model, the effect of different caffeinated drinks was observed in mice exercising ad libitum. During 2 days, the mice received: 1) pure anhydrous caffeine 0.125 mg/mL (PC), 2) cola drink (CC), and 3) caffeine-taurine-glucuronolactone drink (CTG), intercalating wash-out periods of 2 days, receiving pure water.
Results: The distance run during the periods of water ingestion was significantly lower than during the periods of stimulant drinks ingestion: PC (5.6±1.3 km; p = 0.02), of CC ingestion (7.6±0.6 km; p = 0.001), and of CTG ingestion (8.3±1.6 km; p = 0.009). The performances when ingesting the three caffeinated drinks do not follow a dose-response curve.
Conclusions: The model described here was able to measure the effect of caffeine intake on voluntary exercise of mice. The sensitivity of the model to the effect of caffeine needs to be further validated. The action of each component of the drinks on exercise performance needs to be clarified in future research. The present model is adequate for such investigation.
Key words: Exercise; caffeine; energy drinks; running |
| first_indexed | 2024-12-22T10:25:21Z |
| format | Article |
| id | doaj.art-137cbae6b9f74e7a98492103e9d53d2e |
| institution | Directory Open Access Journal |
| issn | 0101-5575 2357-9730 |
| language | English |
| last_indexed | 2024-12-22T10:25:21Z |
| publishDate | 2017-12-01 |
| publisher | Hospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS) |
| record_format | Article |
| series | Clinical and Biomedical Research |
| spelling | doaj.art-137cbae6b9f74e7a98492103e9d53d2e2022-12-21T18:29:29ZengHospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS)Clinical and Biomedical Research0101-55752357-97302017-12-0137435145The effect of caffeine supplementation on exercise performance evaluated by a novel animal modelRoberto Pacheco da Silva0Denis Martinez1Cintia Zappe Fiori2Kelly Silveira da Silva Bueno3Jhoana Mercedes Uribe Ramos4Renata Schenkel KaminskiMarcia Kraide FischerLeticia Maria Tedesco SilvaJuliana Neves GiordaniJuliana Heitich BrendlerJuliana Langendorf da Costa VieiraYasmim de Freitas DiasLaura Martinewski de OliveiraChaiane Facco PiccinEmerson Ferreira Martins1Graduate Program in Cardiology and Cardiovascular Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Interdisciplinary Sleep Research Laboratory, HCPA, Brazil.Graduate Program in Cardiology and Cardiovascular Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Graduate Program in Medical Sciences, UFRGS, Porto Alegre, RS, Brazil. Cardiology Unit, Hospital de Clínicas de Porto Alegre (HCPA), UFRGS, Brazil. Interdisciplinary Sleep Research Laboratory, HCPA, Brazil.Graduate Program in Cardiology and Cardiovascular Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Interdisciplinary Sleep Research Laboratory, HCPA, Brazil.Graduate Program in Cardiology and Cardiovascular Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Interdisciplinary Sleep Research Laboratory, HCPA, Brazil.Graduate Program in Medical Sciences, UFRGS, Porto Alegre, RS, Brazil. Interdisciplinary Sleep Research Laboratory, HCPA, Brazil.Introduction: Caffeinated drinks are used for improve performance. Animal models represent investigational strategy that circumvents most of the drawbacks of research in humans, including motivational factors and the placebo effect. No animal model that could test whether different forms of administering caffeine affect exercise propensity was found in the literature. Methods: An animal model of grouped voluntary exercise was tested. Two-month-old male C57/bl mice were housed in a cage fitted with one running wheel and a monitoring system. Six animals per cage were introduced individually. To assess the sensitivity of the model, the effect of different caffeinated drinks was observed in mice exercising ad libitum. During 2 days, the mice received: 1) pure anhydrous caffeine 0.125 mg/mL (PC), 2) cola drink (CC), and 3) caffeine-taurine-glucuronolactone drink (CTG), intercalating wash-out periods of 2 days, receiving pure water. Results: The distance run during the periods of water ingestion was significantly lower than during the periods of stimulant drinks ingestion: PC (5.6±1.3 km; p = 0.02), of CC ingestion (7.6±0.6 km; p = 0.001), and of CTG ingestion (8.3±1.6 km; p = 0.009). The performances when ingesting the three caffeinated drinks do not follow a dose-response curve. Conclusions: The model described here was able to measure the effect of caffeine intake on voluntary exercise of mice. The sensitivity of the model to the effect of caffeine needs to be further validated. The action of each component of the drinks on exercise performance needs to be clarified in future research. The present model is adequate for such investigation. Key words: Exercise; caffeine; energy drinks; runninghttp://seer.ufrgs.br/index.php/hcpa/article/view/74773Exercisecaffeineenergy drinksrunning |
| spellingShingle | Roberto Pacheco da Silva Denis Martinez Cintia Zappe Fiori Kelly Silveira da Silva Bueno Jhoana Mercedes Uribe Ramos Renata Schenkel Kaminski Marcia Kraide Fischer Leticia Maria Tedesco Silva Juliana Neves Giordani Juliana Heitich Brendler Juliana Langendorf da Costa Vieira Yasmim de Freitas Dias Laura Martinewski de Oliveira Chaiane Facco Piccin Emerson Ferreira Martins The effect of caffeine supplementation on exercise performance evaluated by a novel animal model Clinical and Biomedical Research Exercise caffeine energy drinks running |
| title | The effect of caffeine supplementation on exercise performance evaluated by a novel animal model |
| title_full | The effect of caffeine supplementation on exercise performance evaluated by a novel animal model |
| title_fullStr | The effect of caffeine supplementation on exercise performance evaluated by a novel animal model |
| title_full_unstemmed | The effect of caffeine supplementation on exercise performance evaluated by a novel animal model |
| title_short | The effect of caffeine supplementation on exercise performance evaluated by a novel animal model |
| title_sort | effect of caffeine supplementation on exercise performance evaluated by a novel animal model |
| topic | Exercise caffeine energy drinks running |
| url | http://seer.ufrgs.br/index.php/hcpa/article/view/74773 |
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