Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women
Background:Tissue resident memory T cells (TrM) provide an enhanced response against infection at mucosal surfaces, yet their function has not been extensively studied in humans, including the female genital tract (FGT). Methods: Using polychromatic flow cytometry, we studied TrM cells, defined as...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Case Western Reserve University
2017-01-01
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| Series: | Pathogens and Immunity |
| Subjects: | |
| Online Access: | https://paijournal.com/index.php/paijournal/article/view/166 |
| _version_ | 1818546808132993024 |
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| author | David C. Moylan Paul A. Goepfert Mirjam-Colette Kempf Michael S. Saag Holly E. Richter Jiri Mestecky Steffanie Sabbaj |
| author_facet | David C. Moylan Paul A. Goepfert Mirjam-Colette Kempf Michael S. Saag Holly E. Richter Jiri Mestecky Steffanie Sabbaj |
| author_sort | David C. Moylan |
| collection | DOAJ |
| description | Background:Tissue resident memory T cells (TrM) provide an enhanced response against infection at mucosal surfaces, yet their function has not been extensively studied in humans, including the female genital tract (FGT).
Methods: Using polychromatic flow cytometry, we studied TrM cells, defined as CD62L-CCR7-CD103+CD69+ CD4+ and CD8+ T cells in mucosa-derived T cells from healthy and HIV-positive women.
Results: We demonstrate that TrM are present in the FGT of healthy and HIV-positive women. The expression of the mucosal retention receptor, CD103, from HIV-positive women was reduced compared to healthy women and was lowest in women with CD4 counts < 500 cells/mm3. Furthermore, CD103 expression on mucosa-derived CD8+ T cells correlated with antigen-specific IFN-γ production by mucosal CD4+ T cells and was inversely correlated with T-bet from CD8+CD103+ mucosa-derived T cells.
Conclusions: These data suggest that CD4+ T cells, known to be impaired during HIV-1 infection and necessary for the expression of CD103 in murine models, may play a role in the expression of CD103 on resident T cells from the human FGT. |
| first_indexed | 2024-12-12T07:58:11Z |
| format | Article |
| id | doaj.art-1380b56cadd54ea185106b344ec7bcd8 |
| institution | Directory Open Access Journal |
| issn | 2469-2964 |
| language | English |
| last_indexed | 2024-12-12T07:58:11Z |
| publishDate | 2017-01-01 |
| publisher | Case Western Reserve University |
| record_format | Article |
| series | Pathogens and Immunity |
| spelling | doaj.art-1380b56cadd54ea185106b344ec7bcd82022-12-22T00:32:13ZengCase Western Reserve UniversityPathogens and Immunity2469-29642017-01-011237138910.20411/pai.v1i2.16639Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive womenDavid C. Moylan0Paul A. Goepfert1Mirjam-Colette Kempf2Michael S. Saag3Holly E. Richter4Jiri Mestecky5Steffanie Sabbaj6Departments of Medicine, University of Alabama at Birmingham, Birmingham, ALDepartments of Medicine, University of Alabama at Birmingham, Birmingham, AL; Department of Microbiology, University of Alabama at Birmingham, Birmingham, ALSchool of Nursing and Department of Health Behavior, University of Alabama at Birmingham, Birmingham, ALDepartments of Medicine, University of Alabama at Birmingham, Birmingham, ALObstetrics and Gynecology, University of Alabama at Birmingham, Birmingham, ALDepartment of Microbiology, University of Alabama at Birmingham, Birmingham, AL1Departments of Medicine, University of Alabama at Birmingham, Birmingham, ALBackground:Tissue resident memory T cells (TrM) provide an enhanced response against infection at mucosal surfaces, yet their function has not been extensively studied in humans, including the female genital tract (FGT). Methods: Using polychromatic flow cytometry, we studied TrM cells, defined as CD62L-CCR7-CD103+CD69+ CD4+ and CD8+ T cells in mucosa-derived T cells from healthy and HIV-positive women. Results: We demonstrate that TrM are present in the FGT of healthy and HIV-positive women. The expression of the mucosal retention receptor, CD103, from HIV-positive women was reduced compared to healthy women and was lowest in women with CD4 counts < 500 cells/mm3. Furthermore, CD103 expression on mucosa-derived CD8+ T cells correlated with antigen-specific IFN-γ production by mucosal CD4+ T cells and was inversely correlated with T-bet from CD8+CD103+ mucosa-derived T cells. Conclusions: These data suggest that CD4+ T cells, known to be impaired during HIV-1 infection and necessary for the expression of CD103 in murine models, may play a role in the expression of CD103 on resident T cells from the human FGT.https://paijournal.com/index.php/paijournal/article/view/166Tissue Resident Memory T cellsfemale genital tractHIV-1CD103mucosal immunityCD8+ T cellsCD4 T cells |
| spellingShingle | David C. Moylan Paul A. Goepfert Mirjam-Colette Kempf Michael S. Saag Holly E. Richter Jiri Mestecky Steffanie Sabbaj Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women Pathogens and Immunity Tissue Resident Memory T cells female genital tract HIV-1 CD103 mucosal immunity CD8+ T cells CD4 T cells |
| title | Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women |
| title_full | Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women |
| title_fullStr | Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women |
| title_full_unstemmed | Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women |
| title_short | Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women |
| title_sort | diminished cd103 aeb7 expression on resident t cells from the female genital tract of hiv positive women |
| topic | Tissue Resident Memory T cells female genital tract HIV-1 CD103 mucosal immunity CD8+ T cells CD4 T cells |
| url | https://paijournal.com/index.php/paijournal/article/view/166 |
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