Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species
Oulema melanopus (Linnaeus, 1758) and Oulema duftschmidi (Redtenbacher, 1874) (Coleoptera: Chrysomelidae) are two native West Palaearctic species developing on various cultivated and wild grasses. Along with O. obscura they are considered to be secondary pests of cereal crops. However, local outbrea...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2020-12-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-202001-0052_distinct_barcodes_for_the_cereal_leaf_beetles_oulema_melanopus_and_oulema_duftschmidi_coleoptera_chrysomelida.php |
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| author | Julie LEROY Jean-David CHAPELIN-VISCARDI Guénaëlle GENSON Julien HARAN Éric PIERRE Jean-Claude STREITO |
| author_facet | Julie LEROY Jean-David CHAPELIN-VISCARDI Guénaëlle GENSON Julien HARAN Éric PIERRE Jean-Claude STREITO |
| author_sort | Julie LEROY |
| collection | DOAJ |
| description | Oulema melanopus (Linnaeus, 1758) and Oulema duftschmidi (Redtenbacher, 1874) (Coleoptera: Chrysomelidae) are two native West Palaearctic species developing on various cultivated and wild grasses. Along with O. obscura they are considered to be secondary pests of cereal crops. However, local outbreaks have been recorded recently and their status as secondary pests may evolve, especially as the use of broad-spectrum insecticides is now greatly reduced. Oulema melanopus and O. duftschmidi are considered to be sibling species. They are morphologically very close and difficult to distinguish from each other, which makes it difficult to study them. We tested the reliability of the standard barcode fragment (COI) for distinguishing between these species. A total of 92 samples of the two species, covering the majority of their natural range, was sequenced for the barcode fragment and inter- and intraspecific genetic distances were estimated. Our results confirm those of Bezděk & Baselga (2015, Acta Entomol. Mus. Nat. Prag. 55: 273-304) in that this marker cannot differentiate between all the species of the Oulema melanopus complex, which in the Mediterranean basin contains several described and possibly some undescribed cryptic species. However, this marker may be useful in an agricultural context in areas where only O. melanopus and O. duftschmidi occur (such as in cereal crops in France) where it can be used to reliably and rapidly separate all stages of these two taxa and can therefore help in studying their ecology and dynamics. |
| first_indexed | 2024-12-19T05:53:04Z |
| format | Article |
| id | doaj.art-138461b479404b79bde02e1c7c7bf58b |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-19T05:53:04Z |
| publishDate | 2020-12-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-138461b479404b79bde02e1c7c7bf58b2022-12-21T20:33:32ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292020-12-01117149050310.14411/eje.2020.052eje-202001-0052Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling speciesJulie LEROY0Jean-David CHAPELIN-VISCARDI1Guénaëlle GENSON2Julien HARAN3Éric PIERRE4Jean-Claude STREITO5Laboratoire d'Éco-Entomologie, 5 rue Antoine Mariotte, 45000 Orléans, France; e-mails: leroy@laboratoireecoentomologie.com, chapelinviscardi@laboratoireecoentomologie.comLaboratoire d'Éco-Entomologie, 5 rue Antoine Mariotte, 45000 Orléans, France; e-mails: leroy@laboratoireecoentomologie.com, chapelinviscardi@laboratoireecoentomologie.comCBGP, INRAE, CIRAD, IRD, Institut Agro, Univ Montpellier, Montpellier, France; e-mails: guenaelle.genson@inrae.fr, julien.haran@cirad.fr, eric.pierre@inrae.fr, jean-claude.streito@inrae.frCBGP, INRAE, CIRAD, IRD, Institut Agro, Univ Montpellier, Montpellier, France; e-mails: guenaelle.genson@inrae.fr, julien.haran@cirad.fr, eric.pierre@inrae.fr, jean-claude.streito@inrae.frCBGP, INRAE, CIRAD, IRD, Institut Agro, Univ Montpellier, Montpellier, France; e-mails: guenaelle.genson@inrae.fr, julien.haran@cirad.fr, eric.pierre@inrae.fr, jean-claude.streito@inrae.frCBGP, INRAE, CIRAD, IRD, Institut Agro, Univ Montpellier, Montpellier, France; e-mails: guenaelle.genson@inrae.fr, julien.haran@cirad.fr, eric.pierre@inrae.fr, jean-claude.streito@inrae.frOulema melanopus (Linnaeus, 1758) and Oulema duftschmidi (Redtenbacher, 1874) (Coleoptera: Chrysomelidae) are two native West Palaearctic species developing on various cultivated and wild grasses. Along with O. obscura they are considered to be secondary pests of cereal crops. However, local outbreaks have been recorded recently and their status as secondary pests may evolve, especially as the use of broad-spectrum insecticides is now greatly reduced. Oulema melanopus and O. duftschmidi are considered to be sibling species. They are morphologically very close and difficult to distinguish from each other, which makes it difficult to study them. We tested the reliability of the standard barcode fragment (COI) for distinguishing between these species. A total of 92 samples of the two species, covering the majority of their natural range, was sequenced for the barcode fragment and inter- and intraspecific genetic distances were estimated. Our results confirm those of Bezděk & Baselga (2015, Acta Entomol. Mus. Nat. Prag. 55: 273-304) in that this marker cannot differentiate between all the species of the Oulema melanopus complex, which in the Mediterranean basin contains several described and possibly some undescribed cryptic species. However, this marker may be useful in an agricultural context in areas where only O. melanopus and O. duftschmidi occur (such as in cereal crops in France) where it can be used to reliably and rapidly separate all stages of these two taxa and can therefore help in studying their ecology and dynamics.https://www.eje.cz/artkey/eje-202001-0052_distinct_barcodes_for_the_cereal_leaf_beetles_oulema_melanopus_and_oulema_duftschmidi_coleoptera_chrysomelida.phpcoleopterachrysomelidaeoulemacereal leaf beetlesdna barcodecoiidentificationcrop pestseurope |
| spellingShingle | Julie LEROY Jean-David CHAPELIN-VISCARDI Guénaëlle GENSON Julien HARAN Éric PIERRE Jean-Claude STREITO Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species European Journal of Entomology coleoptera chrysomelidae oulema cereal leaf beetles dna barcode coi identification crop pests europe |
| title | Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species |
| title_full | Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species |
| title_fullStr | Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species |
| title_full_unstemmed | Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species |
| title_short | Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species |
| title_sort | distinct barcodes for the cereal leaf beetles oulema melanopus and oulema duftschmidi coleoptera chrysomelidae two syntopical sibling species |
| topic | coleoptera chrysomelidae oulema cereal leaf beetles dna barcode coi identification crop pests europe |
| url | https://www.eje.cz/artkey/eje-202001-0052_distinct_barcodes_for_the_cereal_leaf_beetles_oulema_melanopus_and_oulema_duftschmidi_coleoptera_chrysomelida.php |
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