T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice
Introduction: Sambucus is a genus of the Adoxaceae family and has been widely used as a food and medicinal source. In this study, we aimed to evaluate the effects of S. javanica extracts toward T cells regulation in the 7,12-dimethylbenzene (a) anthracene (DMBA)-exposed mice. Methods: Thirty mice we...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2020-10-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-9-408.pdf |
| _version_ | 1828128913420713984 |
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| author | Wira Eka Putra A. Rofiqi Maulana Arfan Tri Kusuma Ramadhan Muhaimin Rifa’i |
| author_facet | Wira Eka Putra A. Rofiqi Maulana Arfan Tri Kusuma Ramadhan Muhaimin Rifa’i |
| author_sort | Wira Eka Putra |
| collection | DOAJ |
| description | Introduction: Sambucus is a genus of the Adoxaceae family and has been widely used as a food and medicinal source. In this study, we aimed to evaluate the effects of S. javanica extracts toward T cells regulation in the 7,12-dimethylbenzene (a) anthracene (DMBA)-exposed mice. Methods: Thirty mice were used in this experiment which divided into six treatment groups and five times repetition. Three-month-old BALB/c mice were administrated with 2.8 mg.kg-1 BW of DMBA as carcinogen agent for ten times within four weeks, then continuously treated with 400 and 800 mg.kg-1 BW of S. javanica berries leaves extracts daily for two weeks. Treatment groups in this study were vehicle as negative control, DMBA 2.8 mg.kg-1 BW as positive control; BD1 group, DMBA 2.8 mg.kg-1 + berries extracts 400 mg.kg-1 BW; BD2 group, DMBA 2.8 mg.kg-1 + berries extracts 800 mg.kg-1 BW; LD1 group, DMBA 2.8 mg.kg-1 + leaves extract 400 mg.kg1 BW; and LD2 group, DMBA 2.8 mg.kg-1 + leaves extract 800 mg.kg-1 BW. The relative number of CD4+CD25+CD62L+ cells and CD4+CD62L+ cells were measured and analyzed by using flow cytometer. Results: DMBA induction decreased the relative number of CD4+CD25+CD62L+ cells while the leaves extract of S. javanica significantly increased their expression. Furthermore, DMBA promoted the relative number of CD4+CD62L+ cells expression while leaves and berries extracts of S. javanica sharply suppressed its expression. Conclusion: The results suggest that, S. javanica extracts may exert their medicinal properties by modulating T cells regulation in DMBA-induced mice. |
| first_indexed | 2024-04-11T16:11:32Z |
| format | Article |
| id | doaj.art-1387eabdd9fa4c3d9b44f3fa2a9cc5c8 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-04-11T16:11:32Z |
| publishDate | 2020-10-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-1387eabdd9fa4c3d9b44f3fa2a9cc5c82022-12-22T04:14:39ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042020-10-019440841110.34172/jhp.2020.51jhp-2313T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed miceWira Eka Putra0A. Rofiqi Maulana1Arfan Tri Kusuma Ramadhan2Muhaimin Rifa’i3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, IndonesiaDepartment of Statistics, Faculty of Mathematics and Natural Sciences, Brawijaya University, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, IndonesiaIntroduction: Sambucus is a genus of the Adoxaceae family and has been widely used as a food and medicinal source. In this study, we aimed to evaluate the effects of S. javanica extracts toward T cells regulation in the 7,12-dimethylbenzene (a) anthracene (DMBA)-exposed mice. Methods: Thirty mice were used in this experiment which divided into six treatment groups and five times repetition. Three-month-old BALB/c mice were administrated with 2.8 mg.kg-1 BW of DMBA as carcinogen agent for ten times within four weeks, then continuously treated with 400 and 800 mg.kg-1 BW of S. javanica berries leaves extracts daily for two weeks. Treatment groups in this study were vehicle as negative control, DMBA 2.8 mg.kg-1 BW as positive control; BD1 group, DMBA 2.8 mg.kg-1 + berries extracts 400 mg.kg-1 BW; BD2 group, DMBA 2.8 mg.kg-1 + berries extracts 800 mg.kg-1 BW; LD1 group, DMBA 2.8 mg.kg-1 + leaves extract 400 mg.kg1 BW; and LD2 group, DMBA 2.8 mg.kg-1 + leaves extract 800 mg.kg-1 BW. The relative number of CD4+CD25+CD62L+ cells and CD4+CD62L+ cells were measured and analyzed by using flow cytometer. Results: DMBA induction decreased the relative number of CD4+CD25+CD62L+ cells while the leaves extract of S. javanica significantly increased their expression. Furthermore, DMBA promoted the relative number of CD4+CD62L+ cells expression while leaves and berries extracts of S. javanica sharply suppressed its expression. Conclusion: The results suggest that, S. javanica extracts may exert their medicinal properties by modulating T cells regulation in DMBA-induced mice.http://herbmedpharmacol.com/PDF/jhp-9-408.pdfdmbanaive t cellsregulatory t cellss. javanica |
| spellingShingle | Wira Eka Putra A. Rofiqi Maulana Arfan Tri Kusuma Ramadhan Muhaimin Rifa’i T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice Journal of HerbMed Pharmacology dmba naive t cells regulatory t cells s. javanica |
| title | T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice |
| title_full | T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice |
| title_fullStr | T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice |
| title_full_unstemmed | T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice |
| title_short | T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice |
| title_sort | t cells regulation modulated by sambucus javanica extracts in dmba exposed mice |
| topic | dmba naive t cells regulatory t cells s. javanica |
| url | http://herbmedpharmacol.com/PDF/jhp-9-408.pdf |
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